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OP104L Anti-p53 (Ab-11) (Pantropic) Mouse mAb (PAb1802)

Übersicht

Key Spec Table

Species ReactivityHostAntibody Type
H, MMMonoclonal Antibody

Preis & Verfügbarkeit

Bestellnummer VerfügbarkeitVerpackung St./Pkg. Preis Menge
OP104L-100UG
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      Kst.-Ampulle 100 μg
      Preis nicht abrufbar
      Die Mindestmenge muss ein Vielfaches sein von
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      Description
      OverviewRecognizes the ~53 kDa wild-type and mutant p53 protein in immunoblotting. Recognizes only the wild-type p53 protein in immunoprecipitation.
      Catalogue NumberOP104L
      Brand Family Calbiochem®
      References
      ReferencesLevine, A.J. 1997. Cell 88, 323.
      Miller, S.D., et al. 1997. Mol. Cell. Biol. 17, 2194.
      Pellegata, N.S., et al. 1996. Proc. Natl. Acad. Sci. USA 93, 15209.
      Sturzbecher, H.-W., et al. 1996. EMBO J. 15, 1992.
      Argarwal, M.L., et al. 1995. Proc. Natl. Acad. Sci. USA 92, 8493.
      Götz, C. and Montenarh, M. 1995. Int. J. Oncology 6, 1129.
      Reed, M. et al. 1995. Proc. Natl. Acad. Sci. USA 92, 9455.
      Waldman, T., et al. 1995. Cancer Res. 55, 5187.
      Clarke, A.R., et al. 1994. Oncogene 9, 1767.
      Cho, Y., et al. 1994. Science 265, 346.
      El-Deiry, W.S., et al. 1994. Cancer Res. 54, 1169.
      Greenblatt, M.S., et al. 1994. Cancer Res. 54, 4855.
      Lane, D.P. 1992. Nature 358, 15.
      Product Information
      FormLyophilized
      FormulationLyophilized from a volatile buffer, 100 µg BSA.
      Negative controlSK-OV-3 cells
      Positive controlA431 or SK-BR-3 cells
      PreservativeNone
      Quality LevelMQ100
      Applications
      Application ReferencesImmunoblotting, Immunoprecipitation Banks, L., et al. 1986. Eur. J. Biochem. 159, 529.
      Key Applications Immunoblotting (Western Blotting)
      Immunoprecipitation
      Not Frozen Sections
      Not Immunofluorescence
      Not Paraffin Sections
      Application NotesImmunoblotting (2.5 µg/ml, chemiluminescence, see application references)
      Immunoprecipitation (1 µg/reaction, see comments, see application references)
      Frozen Sections (not recommended)
      Immunofluorescence (not recommended)
      Paraffin Sections (not recommended)
      Application CommentsThis antibody will immunoprecipitate wild-type p53 only. This antibody will stain methanol-fixed cells. Antibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogenrecombinant, human p53 protein
      ImmunogenHuman
      Epitopewithin amino acids 306-393 of human p53
      ClonePAb1802
      HostMouse
      IsotypeIgG₁
      Species Reactivity
      • Human
      • Mouse
      Antibody TypeMonoclonal Antibody
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Do not freeze Ok to freeze
      Special InstructionsReconstitute the lyophilized antibody with sterile PBS, pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4, to yield a final concentration of 100 µg/ml; product will be more stable if 0.1% sodium azide is included. Lyophilized antibodies should be reconstituted at 4°C by gradual addition of solvent over a period of 1-2 h with occasional gentle mixing.
      Global Trade Item Number
      Bestellnummer GTIN
      OP104L-100UG 04055977224917