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Anti-dimethyl PDCD4 (Arg110), clone 3E7, Cat. No. MABC1746, is a mouse monoclonal antibody that detects Programmed cell death protein 4 and is tested for use in Immunocytochemistry and Western Blotting.
More>>Anti-dimethyl PDCD4 (Arg110), clone 3E7, Cat. No. MABC1746, is a mouse monoclonal antibody that detects Programmed cell death protein 4 and is tested for use in Immunocytochemistry and Western Blotting. Less<<
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Übersicht
Replacement Information
Description
Catalogue Number
MABC1746-25UG
Description
Anti-dimethyl PDCD4 (Arg110) Antibody, clone 3E7
Alternate Names
Programmed cell death protein 4
Neoplastic transformation inhibitor protein
Nuclear antigen H731-like
Protein 197/15a
Background Information
Programmed cell death protein 4 (UniProt: Q53EL6; also known as Neoplastic transformation inhibitor protein, Nuclear antigen H731-like, Protein 197/15a, PDCD4) is encoded by the PDCD4 (also known as H731) gene (Gene ID: 27250) in human. PDCD4 is a tumor-suppressor protein that is shown to be upregulated during apoptosis. It affects transcription, translation, and signal transduction to suppress tumor growth. Its levels are upregulated in proliferative cells and lower levels are reported in lung cancer and colon carcinoma. Its levels are also shown to be down regulated in transitional cell carcinoma. PDCD4 is reported to shuttle between the nucleus and cytoplasm but is predominantly nuclear under normal growth conditions and when phosphorylated at serine 457. It contains two nuclear localization signal sequences (aa 58-64 and 241-250). PDCD4 can undergo phosphorylation at serine 67 by Ribosomal protein S6 kinase beta-1 (RPS6KB1) in response to mitogens, which promotes its proteasomal degradation. PDCD4 can undergo methylation at arginine 110 that enhances its interaction with elF4A. The methylated form of PDCD4 is reported to promote tumor viability during nutrient deprivation, which allows tumors to grow more aggressively. This methylation of arginine 110 can reinforce the interaction between the MA3 domains and eIF4A thereby forming a stronger interaction and may also make PDCD4 MA3 domains more accessible for interaction with eIF4A. (Ref.: Goke, R., et al. (2004). Ann. NY Acad. Sci. 1014; 220-1; Fischer, N., et al. (2012). Biochem. Biophys. Res. Commun. 417(6); 29-34; Fay, MM., et al. (2014). J. Biol. Chem. 289(25); 17541-17552).
References
Product Information
Format
Purified
Presentation
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Anti-dimethyl PDCD4 (Arg110), clone 3E7, Cat. No. MABC1746, is a mouse monoclonal antibody that detects Programmed cell death protein 4 and is tested for use in Immunocytochemistry and Western Blotting.
Key Applications
Immunocytochemistry
Western Blotting
Application Notes
Western Blotting Analysis: A representative lot detected dimethyl PDCD4 (Arg110) in Western Blotting applications (Fay, M.M., et. al. (2014). J Biol Chem. 289(25):17541-52).
Immunocytochemistry Analysis: A representative lot detected dimethyl PDCD4 (Arg110) in Immunocytochemistry applications (Fay, M.M., et. al. (2014). J Biol Chem. 289(25):17541-52).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Biological Information
Immunogen
KLH-conjugated linear peptide corresponding to 18 amino acids from the N-terminal half of human Programmed cell death protein 4 with Arg 110 symmetrically dimethylated.
Epitope
N-terminal half
Clone
3E7
Concentration
1 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Host
Mouse
Specificity
Clone 3E7 is a mouse monoclonal antibody that detects Programmed cell death protein 4 (PDCD4) with symmetrically dimethylated Arg 10. It targets an epitope within 18 amino acids from the N-terminal half.
76 kDa observed; 51.73 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance
Evaluated by Western Blotting in HEK293 cells transfected with GFP-PDCD4.
Western Blotting Analysis: 2 µg/mL of this antibody detected Programmed cell death protein 4 (PDCD4) in HEK293 cells transfected with GFP-PDCD4, but not in cells transfected with GFP-PDCD4+Adenosine dialdehyde (AdOx; methylation inhibited), or with GFP-PDCD4 with R110K mutation.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 1 year at +2°C to +8°C from date of receipt.
Enhanced arginine methylation of programmed cell death 4 protein during nutrient deprivation promotes tumor cell viability. Fay MM, Clegg JM, Uchida KA, Powers MA, Ullman KS. J Biol Chem
289(25)
17541-52
2014
The role of programmed cell death 4 (PDCD4) in tumor biology is context-dependent. PDCD4 is described as a tumor suppressor, but its coexpression with protein arginine methyltransferase 5 (PRMT5) promotes accelerated tumor growth. Here, we report that PDCD4 is methylated during nutrient deprivation. Methylation occurs because of increased stability of PDCD4 protein as well as increased activity of PRMT5 toward PDCD4. During nutrient deprivation, levels of methylated PDCD4 promote cell viability, which is dependent on an enhanced interaction with eIF4A. Upon recovery from nutrient deprivation, levels of methylated PDCD4 are regulated by phosphorylation, which controls both the localization and stability of methylated PDCD4. This study reveals that, in response to particular environmental cues, the role of PDCD4 is up-regulated and is advantageous for cell viability. These findings suggest that the methylated form of PDCD4 promotes tumor viability during nutrient deprivation, ultimately allowing the tumor to grow more aggressively.
