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OP10F Anti-c-Myc (Ab-1) Mouse mAb (9E10) Fluorescein Conjugate

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OP10F
  
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      Übersicht

      Replacement Information

      Key Spec Table

      Species ReactivityHostAntibody Type
      H, M, RMMonoclonal Antibody
      Description
      Overview

      This product has been discontinued.



      Recognizes the ~60-67 kDa c-Myc protein in HL60 cells and lung carcinoma tissue.
      Catalogue NumberOP10F
      Brand Family Calbiochem®
      References
      ReferencesLeGouy, E., et al. 1987. In Nuclear Oncogenes, Cold Spring Harbor Laboratory, 144.
      Cole, M.D. 1986. Ann. Rev. Gen. 20, 361.
      Nisen, P.D., et al. 1986. Cancer Res. 46, 6217.
      Nau, M.M., et al. 1985. Nature 318, 69.
      Persson, H., et al. 1984. Science 225, 687.
      Alitalo, K., et al. 1983. Proc. Natl. Acad. Sci. USA 80, 1707.
      Product Information
      FormLiquid
      FormulationIn 50 mM sodium phosphate buffer, 0.2% gelatin.
      Negative controlHT1080 cells
      Positive controlHL-60 cells or lung carcinoma
      Preservative≤0.1% sodium azide
      Quality LevelMQ100
      Applications
      Application ReferencesEpitope Tagging Munro, S., et al. 1986. Cell 46, 291. Immunoblotting Evan, G.I., et al. 1985. Mol. Cell. Biol. 5, 3610.
      Key Applications Flow Cytometry
      Immunofluorescence
      Application NotesFlow Cytometry (20 µg/ml)
      Frozen Sections (use Cat. No. OP10, OP10L, or OP10T)
      Immunoblotting (use Cat. No. OP10, OP10L, or OP10T)
      Immunofluorescence (1-5 µg/ml)
      Immunoprecipitation (use Cat. No. OP10, OP10L, or OP10T)
      Paraffin Sections (use Cat. No. OP10, OP10L, or OP10T)
      Application CommentsThe c-Myc protein is extremely labile. c-Myc degradation can be as a result of freeze/thaw cycles, thus, we recommend using fresh lysates with a cocktail of protease inhibitors. For immunoblotting, paraffin sections (colorimetric) Cat. No. OP10, OP10L, and OP10F are available. c-Myc is often destroyed during the processing of paraffin sections and can therefore be difficult to detect in this application. This antibody recognizes the c-Myc protein and its cleavage products. The sequence of c-Myc predicts a ~45 kDa protein, but c-Myc migrates under reducing conditions as a ~64-67 kDa band. This antibody will not detect v-Myc but may react weakly to rodent c-Myc when used at high concentrations (10 µg/ml). An extra ~34-40 kDa band may be detected in a immunoblot. May also be used in immunofluorescence as well as the detection and purification of recombinant proteins tagged with the Myc epitope sequence EQKLISEEDL (see application references). The immunogen, c-Myc (Peptide-1) is also available for competition studies (Cat. No. PP06). Antibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogena synthetic peptide (AEEQKLISEEDLLRKRREQLKHKLEQLRNSCA) corresponding to amino acids 408-439 of human c-Myc
      ImmunogenHuman
      Epitopewithin amino acids 410-419
      Clone9E10
      HostMouse
      IsotypeIgG₁
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypeMonoclonal Antibody
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Protect from Light Protect from light
      Do not freeze Yes
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Bestellnummer GTIN
      OP10F 0

      Documentation

      Anti-c-Myc (Ab-1) Mouse mAb (9E10) Fluorescein Conjugate SDB

      Titel

      Sicherheitsdatenblatt (SDB) 

      Anti-c-Myc (Ab-1) Mouse mAb (9E10) Fluorescein Conjugate Analysenzertifikate

      TitelChargennummer
      OP10F

      Literatur

      Übersicht
      LeGouy, E., et al. 1987. In Nuclear Oncogenes, Cold Spring Harbor Laboratory, 144.
      Cole, M.D. 1986. Ann. Rev. Gen. 20, 361.
      Nisen, P.D., et al. 1986. Cancer Res. 46, 6217.
      Nau, M.M., et al. 1985. Nature 318, 69.
      Persson, H., et al. 1984. Science 225, 687.
      Alitalo, K., et al. 1983. Proc. Natl. Acad. Sci. USA 80, 1707.

      Broschüre

      Titel
      Caspases and other Apoptosis Related Tools Brochure
      Datenblatt

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision02-October-2007 RFH
      ApplicationFlow Cytometry (20 µg/ml)
      Frozen Sections (use Cat. No. OP10, OP10L, or OP10T)
      Immunoblotting (use Cat. No. OP10, OP10L, or OP10T)
      Immunofluorescence (1-5 µg/ml)
      Immunoprecipitation (use Cat. No. OP10, OP10L, or OP10T)
      Paraffin Sections (use Cat. No. OP10, OP10L, or OP10T)
      DescriptionPurified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with SP2/0 mouse myeloma cells. Conjugated to FITC. Recognizes the ~64-67 kDa (apparent MW) c-Myc protein.
      BackgroundThe v-myc oncogene, initially identified in the MC29 avian retrovirus, causes myelocytomas, carcinomas, sarcomas and lymphomas, and belongs to a family of oncogenes conserved throughout evolution. In humans the family consists of five genes: c-myc, N-myc, R-myc, L-myc, and B-myc. Amplification of the c-myc gene has been found in several types of human tumors including lung, breast and colon carcinomas, while the N-myc gene has been found amplified in neuroblastomas, small cell lung carcinomas and Wilms tumors. L-myc is amplified in small cell lung cancer. Immunological studies have shown that the human c-myc gene gives rise to at least two nuclear phosphoproteins of ~64 kDa and ~67 kDa that exhibit relatively short (30 min) half lives in vivo and exhibit DNA binding properties in vitro.
      HostMouse
      Immunogen speciesHuman
      Immunogena synthetic peptide (AEEQKLISEEDLLRKRREQLKHKLEQLRNSCA) corresponding to amino acids 408-439 of human c-Myc
      Epitopewithin amino acids 410-419
      Clone9E10
      IsotypeIgG₁
      Specieshuman, mouse (weakly), rat (weakly)
      Positive controlHL-60 cells or lung carcinoma
      Negative controlHT1080 cells
      FormLiquid
      FormulationIn 50 mM sodium phosphate buffer, 0.2% gelatin.
      Concentration Label Please refer to vial label for lot-specific concentration
      Preservative≤0.1% sodium azide
      CommentsThe c-Myc protein is extremely labile. c-Myc degradation can be as a result of freeze/thaw cycles, thus, we recommend using fresh lysates with a cocktail of protease inhibitors. For immunoblotting, paraffin sections (colorimetric) Cat. No. OP10, OP10L, and OP10F are available. c-Myc is often destroyed during the processing of paraffin sections and can therefore be difficult to detect in this application. This antibody recognizes the c-Myc protein and its cleavage products. The sequence of c-Myc predicts a ~45 kDa protein, but c-Myc migrates under reducing conditions as a ~64-67 kDa band. This antibody will not detect v-Myc but may react weakly to rodent c-Myc when used at high concentrations (10 µg/ml). An extra ~34-40 kDa band may be detected in a immunoblot. May also be used in immunofluorescence as well as the detection and purification of recombinant proteins tagged with the Myc epitope sequence EQKLISEEDL (see application references). The immunogen, c-Myc (Peptide-1) is also available for competition studies (Cat. No. PP06). Antibody should be titrated for optimal results in individual systems.
      Storage Protect from light
      +2°C to +8°C
      Do Not Freeze Yes
      Toxicity Standard Handling
      ReferencesLeGouy, E., et al. 1987. In Nuclear Oncogenes, Cold Spring Harbor Laboratory, 144.
      Cole, M.D. 1986. Ann. Rev. Gen. 20, 361.
      Nisen, P.D., et al. 1986. Cancer Res. 46, 6217.
      Nau, M.M., et al. 1985. Nature 318, 69.
      Persson, H., et al. 1984. Science 225, 687.
      Alitalo, K., et al. 1983. Proc. Natl. Acad. Sci. USA 80, 1707.
      Application referencesEpitope Tagging Munro, S., et al. 1986. Cell 46, 291. Immunoblotting Evan, G.I., et al. 1985. Mol. Cell. Biol. 5, 3610.

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      Kategorien

      Life Science Research > Antibodies and Assays > Primary Antibodies