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Die folgenden MAPmates™ sollten nicht zusammen analysiert werden: -MAPmates™, die einen unterschiedlichen Assaypuffer erfordern. -Phosphospezifische und MAPmate™ Gesamtkombinationen wie Gesamt-GSK3β und Gesamt-GSK3β (Ser 9). -PanTyr und locusspezifische MAPmates™, z.B. Phospho-EGF-Rezeptor und Phospho-STAT1 (Tyr701). -Mehr als 1 Phospho-MAPmate™ für ein einziges Target (Akt, STAT3). -GAPDH und β-Tubulin können nicht mit Kits oder MAPmates™, die panTyr enthalten, analysiert werden.
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96-Well Plate
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48-602MAG
Buffer Detection Kit for Magnetic Beads
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Detect Tubulin using this rabbit polyclonal antibody, Anti-alpha Tubulin Antibody, nontyrosinated validated for use in western blotting, ELISA & ICC.
More>>Detect Tubulin using this rabbit polyclonal antibody, Anti-alpha Tubulin Antibody, nontyrosinated validated for use in western blotting, ELISA & ICC. Less<<
Anti-alpha Tubulin Antibody, nontyrosinated: SDB (Sicherheitsdatenblätter), Analysenzertifikate und Qualitätszertifikate, Dossiers, Broschüren und andere verfügbare Dokumente.
Microtubules are involved in a wide variety of cellular activities ranging from mitosis and transport events to cell movement and the maintenance of cell shape. Tubulin itself is a globular protein which consists of two polypeptides (alpha and beta tubulin). Alpha and beta tubulin dimers are assembled to 13 protofilaments that form a microtubule of 22 nm diameter. Tyrosine ligase adds a C-terminal tyrosin to monomeric alpha tubulin. Assembled microtubules can be detyrosinated by a cytoskeleton associated carboxypeptidase. Detyrosinated alpha tubulin is referred to as Glu-tubulin. Another post-translational modification of detyrosinated alpha tubulin is C-terminal polyglutamylation which is characteristic for microtubules in neuronal cells and the mitotic spindle.
References
Product Information
Format
Serum
Presentation
Rabbit polyclonal serum in buffer containing serum with 0.05% sodium azide.
Detect Tubulin using this rabbit polyclonal antibody, Anti-alpha Tubulin Antibody, nontyrosinated validated for use in western blotting, ELISA & ICC.
Key Applications
Western Blotting
ELISA
Immunocytochemistry
Application Notes
Western Blotting Analysis: A representative lot from an independent laboratory specifically detected nontyrosinated alpha Tubulin and not tyrosinated alpha Tubulin from purified porcine alpha Tubulin (Gundersen, G. G., et al. (1984). Cell. 38(3):779-89.).
ELISA Analysis: A representative lot from an independent laboratory specifically detected nontyrosinated alpha Tubulin, and not nontyrosinated alpha Tubulin in an indirect ELISA and in a competitive ELISA (Gundersen, G. G., et al. (1984). Cell. 38(3):779-89.).
Immunocytochemistry Analysis: A representative lot from an independent laboratory specifically detected nontyrosinated alpha Tubulin, and not nontyrosinated alpha Tubulin in TC-7 cells in interphase (Gundersen, G. G., et al. (1984). Cell. 38(3):779-89.).
Biological Information
Immunogen
KLH-conjugated linear peptide corresponding to human nontyrosinated alpha Tubulin.
Host
Rabbit
Specificity
This antibody detects the nontyrosinated form of alpha Tubulin.
Species Reactivity
Mouse
Porcine
Human
Rat
Species Reactivity Note
Demonstrated to react with Mouse and Porcine. Predicted to react with Human and Rat based on 100% sequence homology.
Evaluated by Western Blotting in NIH/3T3 cell lysate, which were untreated or treated with Pancreatic Carboxypeptidase (PCA).
Western Blotting Analysis: A 1:2,000 dilution of this antibody detected alpha Tubulin, nontyrosinated in 10 µg of PCA treated NIH/3T3 cell lysate and demonstrated a loss of signal in untreated NIH/3T3 cell lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Distinct populations of microtubules: tyrosinated and nontyrosinated alpha tubulin are distributed differently in vivo. Gundersen, G G, et al. Cell, 38: 779-89 (1984)
1983
A unique post-translational modification of tubulin has previously been described in which a tyrosine residue is reversibly added to the C terminus of the alpha-tubulin subunit. We have prepared peptide antibodies that specifically react (shown by competitive immunoassay and Western blots) with the tyrosinated (Tyr) and nontyrosinated (Glu) forms of alpha-tubulin. Immunofluorescence with these antibodies demonstrated that the distributions of Tyr and Glu tubulin in fixed cells were markedly different. Tyr tubulin was found throughout the interphase network of microtubules and in the metaphase spindle, whereas Glu tubulin was present in a limited subset of interphase microtubules and was absent from the astral fibers of the metaphase spindle. Double immunofluorescence showed that Glu and Tyr microtubules comprised distinct subsets of the total cellular microtubules. These results suggest that tyrosination is involved in the establishment of separate populations of microtubules that may functionally distinct.
