Wenn Sie das Fenster schließen, wird Ihre Konfiguration nicht gespeichert, es sei denn, Sie haben Ihren Artikel in die Bestellung aufgenommen oder zu Ihren Favoriten hinzugefügt.
Klicken Sie auf OK, um das MILLIPLEX® MAP-Tool zu schließen oder auf Abbrechen, um zu Ihrer Auswahl zurückzukehren.
Wählen Sie konfigurierbare Panels & Premixed-Kits - ODER - Kits für die zelluläre Signaltransduktion & MAPmates™
Konfigurieren Sie Ihre MILLIPLEX® MAP-Kits und lassen sich den Preis anzeigen.
Konfigurierbare Panels & Premixed-Kits
Unser breites Angebot enthält Multiplex-Panels, für die Sie die Analyten auswählen können, die am besten für Ihre Anwendung geeignet sind. Unter einem separaten Register können Sie das Premixed-Cytokin-Format oder ein Singleplex-Kit wählen.
Kits für die zelluläre Signaltransduktion & MAPmates™
Wählen Sie gebrauchsfertige Kits zur Erforschung gesamter Signalwege oder Prozesse. Oder konfigurieren Sie Ihre eigenen Kits mit Singleplex MAPmates™.
Die folgenden MAPmates™ sollten nicht zusammen analysiert werden: -MAPmates™, die einen unterschiedlichen Assaypuffer erfordern. -Phosphospezifische und MAPmate™ Gesamtkombinationen wie Gesamt-GSK3β und Gesamt-GSK3β (Ser 9). -PanTyr und locusspezifische MAPmates™, z.B. Phospho-EGF-Rezeptor und Phospho-STAT1 (Tyr701). -Mehr als 1 Phospho-MAPmate™ für ein einziges Target (Akt, STAT3). -GAPDH und β-Tubulin können nicht mit Kits oder MAPmates™, die panTyr enthalten, analysiert werden.
.
Bestellnummer
Bestellinformationen
St./Pkg.
Liste
Dieser Artikel wurde zu Ihren Favoriten hinzugefügt.
Wählen Sie bitte Spezies, Panelart, Kit oder Probenart
Um Ihr MILLIPLEX® MAP-Kit zu konfigurieren, wählen Sie zunächst eine Spezies, eine Panelart und/oder ein Kit.
Custom Premix Selecting "Custom Premix" option means that all of the beads you have chosen will be premixed in manufacturing before the kit is sent to you.
Catalogue Number
Ordering Description
Qty/Pack
List
Dieser Artikel wurde zu Ihren Favoriten hinzugefügt.
Spezies
Panelart
Gewähltes Kit
Menge
Bestellnummer
Bestellinformationen
St./Pkg.
Listenpreis
96-Well Plate
Menge
Bestellnummer
Bestellinformationen
St./Pkg.
Listenpreis
Weitere Reagenzien hinzufügen (MAPmates erfordern die Verwendung eines Puffer- und Detektionskits)
Menge
Bestellnummer
Bestellinformationen
St./Pkg.
Listenpreis
48-602MAG
Buffer Detection Kit for Magnetic Beads
1 Kit
Platzsparende Option Kunden, die mehrere Kits kaufen, können ihre Multiplex-Assaykomponenten in Kunststoffbeuteln anstelle von Packungen erhalten, um eine kompaktere Lagerung zu ermöglichen.
Dieser Artikel wurde zu Ihren Favoriten hinzugefügt.
Das Produkt wurde in Ihre Bestellung aufgenommen
Sie können nun ein weiteres Kit konfigurieren, ein Premixed-Kit wählen, zur Kasse gehen oder das Bestell-Tool schließen.
Anti-acetyl SMC3 (Lys105/106), clone 21A7, Cat. No. MABE1073, is a highly specific mouse monoclonal antibody that targets SMC3 Lys105/106 acetylation and has been tested in Chromatin Immunoprecipitation (ChIP), ChIP-seq, ELISA, and Western Blotting.
More>>Anti-acetyl SMC3 (Lys105/106), clone 21A7, Cat. No. MABE1073, is a highly specific mouse monoclonal antibody that targets SMC3 Lys105/106 acetylation and has been tested in Chromatin Immunoprecipitation (ChIP), ChIP-seq, ELISA, and Western Blotting. Less<<
SDB (Sicherheitsdatenblätter), Analysenzertifikate und Qualitätszertifikate, Dossiers, Broschüren und andere verfügbare Dokumente.
Structural maintenance of chromosomes protein 3 (UniProt Q9UQE7; also known as Bamacan, Basement membrane-associated chondroitin proteoglycan, Chondroitin sulfate proteoglycan 6, Chromosome-associated polypeptide, hCAP, SMC protein 3, SMC-3) is encoded by the SMC3 (also known as BAM, BMH, CDLS3, CSPG6, SMC3L1) gene (Gene ID 9126) in human. Cohesion is a nuclear tripartite complex composed of Rad21 and two structural maintenance of chromosomes proteins, SMC1 and SMC3. Cohesin plays an important role in sister chromatid cohesion, as well as in DNA repair and gene expression regulation. To establish sister chromatid cohesion during S phase, SMC3 is acetylated (on K105 and K106 in human) by two cohesin acetyltransferases (CoATs), ESCO1 and ESCO2, that differ in their N-terminal domains and expression during development and across the cell cycle. ChIP-seq analysis reveals that ESCO1 is recruited by cohesin to over 11,000 chromatin sites occupied by cohesin and CTCF that mediate long-range chromatin interactions and regulate transcription globally. On ther other hand, ESCO2 is infrequently enriched at sites targeted by REST/NRSF (RE1-silencing transcription factor/neuron-restrictive silencer factor) that represses transcription of neuron-specific genes. SMC3 acetylation is thought to induce conformational changes of the dimeric hinge structure during S phase and to allow dimer opening and loading of DNA.
References
Product Information
Format
Purified
Presentation
Purified mouse IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Anti-acetyl SMC3 (Lys105/106), clone 21A7, Cat. No. MABE1073, is a highly specific mouse monoclonal antibody that targets SMC3 Lys105/106 acetylation and has been tested in Chromatin Immunoprecipitation (ChIP), ChIP-seq, ELISA, and Western Blotting.
Key Applications
Chromatin Immunoprecipitation (ChIP)
ChIP-seq
ELISA
Western Blotting
Application Notes
ELISA Analysis: A representative lot bound Lys105/106-diacetylated SMC3 peptide with 2-fold higher affinity than Lys105-monoacetylated SMC3 peptide (Kd = 0.04 and 0.09 µg/mL, respectively), while displaying much reduced affinity toward Lys105-monoacetylated (Kd = 0.73 µg/mL) and little affinity toward nonacetylated (Kd = 2.99 µg/mL) peptide (Rahman, S., et al. (2015). Proc. Natl. Acad. Sci. U. S. A. 112(36):11270-11275).
Chromatin Immunoprecipitation (ChIP) Analysis: A representative lot detected enhanced SMC3 occupancy at target chromatin sites in HDAC8-null HCT116 cells than wild-type HCT-116 cells (Rahman, S., et al. (2015). Proc. Natl. Acad. Sci. U. S. A. 112(36):11270-11275).
ChIP-seq Analysis: A representative lot detected SMC3 chromosome 11 enrichment sites, including 81% of Rad21 target sites and 70% of Esco1 target sites, by ChIP-seq (Rahman, S., et al. (2015). Proc. Natl. Acad. Sci. U. S. A. 112(36):11270-11275).
Western Blotting Analysis: A representative lot detected SMC3 Lys105/106 acetylation in HeLa cells. ESCO1 or ESCO2 depletion by shRNA treatment reduced SMC3 Lys105/106 acetylation level, while dual ESCO1/2 depletion resulted in most profound SMC3 Lys105/106 acetylation reduction (Rahman, S., et al. (2015). Proc. Natl. Acad. Sci. U. S. A. 112(36):11270-11275).
Biological Information
Immunogen
KLH-conjugated linear peptide corresponding to the target region sequence of human/mouse/rat SMC3 containing acetylated Lys105 and Lys106.
Clone
21A7
Concentration
Please refer to lot specific datasheet.
Host
Mouse
Specificity
Clone 21A7 bound Lys105/106-diacetylated SMC3 peptide with 2-fold higher affinity than Lys105-monoacetylated SMC3 peptide (Kd = 0.04 and 0.09 µg/mL, respectively), while displaying much reduced affinity toward Lys105-monoacetylated (Kd = 0.73 µg/mL) and little affinity toward nonacetylated (Kd = 2.99 µg/mL) peptide (Rahman, S., et al. (2015). Proc. Natl. Acad. Sci. U. S. A. 112(36):11270-11275).
Isotype
IgG2bκ
Species Reactivity
Human
Species Reactivity Note
Human. Predicted to react with Bovine, Mouse, Non-Human Primate, Rat, Xenopus based on 100% sequence homology.
~160 kDa observed. 142.0/141.5/141.6/138.4/140.7 kDa (bovine/human/mouse/rat/xenopus) calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance
Evaluated by Western Blotting in human retinal epithelial pigment (REP) cells.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected a reduced SMC3 Lys105/106 acetylation in 10 µg of lysate from N-acetyltransferase ESCO1-knockout human retinal epithelial pigment (REP) cells than in lysate from wild-type REP cells.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
