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ABE1958 Anti-Zic1/2

Anti-Zic1/2, Cat. No. ABE1958, is a highly specific rabbit polyclonal antibody that targets Zinc finger protein ZIC 1 and has been tested in Chromatin Immunoprecipitation (ChIP), Immunocytochemistry, Immunofluorescence, and Western Blotting.

Anti-Zic1/2: SDB (Sicherheitsdatenblätter), Analysenzertifikate und Qualitätszertifikate, Dossiers, Broschüren und andere verfügbare Dokumente.
Research Category: Epigenetics & Nuclear Function Gene Symbol: Zic 1, Zic UniProt Number: P46684
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ABE1958
100 µL  
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      Übersicht

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, MIF, WB, ChIP, ICCRbUnpurifiedPolyclonal Antibody
      Description
      Catalogue NumberABE1958
      DescriptionAnti-Zic1/2
      Alternate Names
      • Zinc finger protein of the cerebellum 1
      Background InformationZinc finger protein ZIC 1 (UniProt: P46684; also known as Zinc finger protein of the cerebellum 1) is encoded by the Zic 1 (also known as Zic) gene (Gene ID: 22771) in murine species. Zic 1 is a member of the GLI C2H2-type zinc-finger protein family. Zic 1 is expressed in the central nervous system and in osteoblasts. A high level expression is seen in the cerebellum, while a low level expression is seen in the olfactory bulb, diencephalon, and brainstem. It acts as a transcriptional activator that is involved in neurogenesis and plays important roles in the early stage of organogenesis of the CNS, as well as during dorsal spinal cord development and maturation of the cerebellum. The C2H2-type 3, 4 and 5 zinc finger domains of Zic 1 are considered to be essential for transcription activation and for Zic 1 interaction with MyoD family inhibitor domain-containing (MDFIC) protein. Zic 1 is also shown to be involved in the spatial distribution of mossy fiber (MF) neurons within the pontine gray nucleus (PGN). It plays a role in the regulation of MF axon pathway choice and promotes MF migration towards ipsilaterally-located cerebellar territories.
      References
      Product Information
      FormatUnpurified
      PresentationRabbit polyclonal serum with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Zic1/2, Cat. No. ABE1958, is a highly specific rabbit polyclonal antibody that targets Zinc finger protein ZIC 1 and has been tested in Chromatin Immunoprecipitation (ChIP), Immunocytochemistry, Immunofluorescence, and Western Blotting.
      Key Applications
      • Immunofluorescence
      • Western Blotting
      • Chromatin Immunoprecipitation (ChIP)
      • Immunocytochemistry
      Application NotesChromatin Immunoprecipitation (ChIP) Analysis: A representative lot detected Zic1/2 in mouse cerebellum (Frank, C.L., et. al. (2015). Nat Neurosci. 18(5):647-56).

      Immunofluorescence Analysis: A representative lot detected Zic1/2 in postmitotic granule cells (Kim, J.Y., et. al. (2003). Dev Biol. 263(1):50-66) and adult mouse cerebellum (Koirala, S., et. al. (2009). J Neurosci. 29(23):7439-49).

      Immunocytochemistry Analysis: A representative lot detected Zic1/2 in Immunocytochemistry applications (Kenney, A.M., et. al. (2000). Mol Cell Biol. 20(23):9055-67).
      Biological Information
      ImmunogenA linear peptide corresponding to 20 amino acids from the C-terminal end of mouse Zic 1 protein.
      EpitopeC-terminus
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityThis polyclonal antibody detects Zic1 protein in mouse and human.
      Species Reactivity
      • Human
      • Mouse
      Species Reactivity NoteHuman, Mouse.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Zic 1
      • Zic
      Purification MethodUnpurified
      UniProt Number
      Molecular Weight~53 kDa observed; 48.33 kDa calculated for Zic1; 54.95 kDa calculated for Zic2. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in human cerebellum tissue lysate.

      Western Blotting Analysis: 1:500 dilution of this antibody detected Zic1/2 in 10 µg of human cerebellum tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.

      Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Bestellnummer GTIN
      ABE1958 04054839168697