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MABC595 Anti-VEGF 165b Antibody, clone 56/1

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MABC595
200 µg  
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      Übersicht

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      M, R, HWB, IHC, ELISAMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABC595
      DescriptionAnti-VEGF 165b Antibody, clone 56/1
      Alternate Names
      • Vascular endothelial growth factor 165b
      • Vascular endothelial growth factor 165
      • Vascular endothelial growth factor A
      • Vascular permeability factor
      Background InformationVEGF (Vascular endothelial growth factor, VEGFA, VEGF-A), a dimeric ligand, is among the most potent angiogenic mitogens. VEGF is secreted by tumor cells and other cells exposed to hypoxia. VEGF is a highly specific mitogen for vascular endothelial cells. Seven VEGF isoforms (a, b, c, d, e, f, g) are generated as a result of alternative splicing from a single VEGF gene. The most commonly studied isoforms are VEGF121, VEGF165, and VEGF189 (f, d and b, respectively) All seven isoforms differ in their molecular mass and in biological properties such as their ability to bind to cell-surface heparan-sulfate proteoglycans. The expression of VEGF is potentiated and is secreted by tumor cells in response to hypoxia, by activated oncogenes, and by a variety of cytokines. VEGF induces angiogenesis as well as permeabilization of blood vessels, and plays a central role in the regulation of vasculogenesis. VEGF expression correlates to the degree of tumor vascularization and with increased metastatic risk. Additionally, VEGF induces endothelial cell proliferation, promotes cell migration, and inhibits apoptosis. Consequently, inhibition of VEGF signaling abrogates the development of a wide variety of tumors.
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-VEGF 165b Antibody, clone 56/1 is validated for use in Western Blotting and Immunohistochemistry and ELISA for the detection of VEGF 165b.
      Key Applications
      • Western Blotting
      • Immunohistochemistry
      • ELISA
      Application NotesWestern Blotting Analysis: 2 µg/mL of a representative lot of this antibody detected VEGF 165b in 10 µg of PC12 cell lysate.

      Immunohistochemistry Analysis: A 1:50 dilution of a representative lot detected detected VEGF 165b in tumor cells of human prostate cancer tissue.

      Western Blotting Analysis: A representative lot detected recombinant VEGF 165b protein (Woolard, J., et al. (2004) CANCER RESEARCH 64:7822–7835).

      Western Blotting Analysis: A representative lot detected VEGF 165b in human eye tissue lysate (Perrin, R.M., et al. (2005) Diabetologia. 48: 2422–2427).

      Immunohistochemistry Analysis: A representative lot detected VEGF 165b in Mouse mesentery tissue (Woolard, J., et al. (2004) CANCER RESEARCH 64:7822–7835).

      Immunohistochemistry Analysis: A representative lot detected VEGF 165b in tumor tissue sections (Peiris-Pagès, M., et al. (2010) Pathol. 222(2): 138–147).

      ELISA: A representative lot of this antibody detected VEGF 165b by ELISA on tumor tissue samples & tumor cell lines (Peiris-Pagès, M., et al. (2010) Pathol. 222(2): 138–147).

      ELISA: A representative lot of this antibody detected VEGF 165b by ELISA on recombinant protein VEGF 165b (Woolard, J., et al. (2004) CANCER RESEARCH 64:7822–7835).
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to the C-terminus of Mouse VEGF 165b.
      EpitopeC-terminus
      Clone56/1
      Concentration1 mg/mL
      HostMouse
      IsotypeIgG1κ
      Species Reactivity
      • Mouse
      • Rat
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • VEGF165b
      • VEGF165
      • VEGFA
      • VEGF
      • VPF
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight~ 22 kDa observed.
      Additional uncharacterized bands may be visible in some lysates.
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in NIH/3T3 cell lysate.

      Western Blotting Analysis: 2 µg/mL of this antibody detected VEGF 165b in 10 µg of NIH/3T3 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size200 µg
      Global Trade Item Number
      Bestellnummer GTIN
      MABC595 04053252983627