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This mouse monoclonal Anti-Tropomyosin alpha-1, clone 3F5E4, Cat. No. MABT1338 is tested for use in Western Blotting, for the detection of Tropomyosin alpha-1 chain.
More>>This mouse monoclonal Anti-Tropomyosin alpha-1, clone 3F5E4, Cat. No. MABT1338 is tested for use in Western Blotting, for the detection of Tropomyosin alpha-1 chain. Less<<
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Übersicht
Replacement Information
Description
Catalogue Number
MABT1338-25UL
Description
Anti-Tropomyosin alpha-1 Antibody, clone 3F5E4
Alternate Names
Tropomyosin alpha-1 chain
Alpha-tropomyosin
Tropomyosin-1
Background Information
Tropomyosin alpha-1 chain (UniProt: P04692; also known as Alpha-tropomyosin, Tropomyosin-1) is encoded by the Tpm1 (also known as Alpha-tm, Tpma) gene (Gene ID: 24851) in rat. Tropomyosins are dimers of coiled-coil proteins that provide stability to actin filaments and regulate access of other actin-binding proteins. Tropomyosin-1 is a cytoskeletal protein that associates with F-actin stress fibers. It binds to actin filaments in muscle and non-muscle cells and plays a central role, in association with the troponin complex, in the calcium dependent regulation of vertebrate striated muscle contraction. It stabilizes actin filaments and regulates the interaction of actin filament with other actin-binding proteins. In non-muscle cells is implicated in stabilizing cytoskeleton actin filaments. Tropomyosin-1 is a homodimeric protein, but can form heterodimer of an alpha (TPM1, TPM3, or TPM4) and a beta (TPM2) chain. Mutations in TPM1 gene have been linked to cardiomyopathy characterized by ventricular hypertrophy and ventricular dilation and impaired systolic function that results in congestive heart failure and arrhythmia. Eight different isoforms of Tropomyosin-1 have been described in rat that are produced by alternative splicing. (Ref.: Schevzov, G., et al. (2011). BioArchitecture, 1 (4); 135-164).
References
Product Information
Format
Purified
Presentation
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Applications
Application
This mouse monoclonal Anti-Tropomyosin alpha-1, clone 3F5E4, Cat. No. MABT1338 is tested for use in Western Blotting, for the detection of Tropomyosin alpha-1 chain.
Key Applications
Western Blotting
Application Notes
Western Blotting Analysis: A representative lot detected Tropomyosin alpha-1 in Western Blotting applications (Schevzov, G., et. al. (2011). Bioarchitecture. 1(4):135-164). Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Biological Information
Immunogen
KLH-conjugated linear peptide corresponding to 24 amino acids from the N-terminal region of rat Tropomyosin alpha-1 chain, isoform 2.
Clone
3F5E4
Concentration
0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Host
Mouse
Specificity
Clone 3F5E4 is a mouse monoclonal antibody that detects Tropomyosin alpha-1 chain. It targets an epitope within 24 amino acids from the N-terminal half corresponding to exon2a region in TPM1 gene.
~34 kDa observed; 32.68 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance
Evaluated by Western Blotting in mouse brain tissue lysates (Neg Ctrl) and mouse stomach tissue lysates (Pos Ctrl).
Western Blotting Analysis: A 1:250 dilution of this antibody detected Tropomyosin alpha-1 in mouse brain tissue lysates (Neg Ctrl) and mouse stomach tissue lysates (Pos Ctrl).
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Tropomyosins are rod-like dimers which form head-to-tail polymers along the length of actin filaments and regulate the access of actin binding proteins to the filaments.1 The diversity of tropomyosin isoforms, over 40 in mammals, and their role in an increasing number of biological processes presents a challenge both to experienced researchers and those new to this field. The increased appreciation that the role of these isoforms expands beyond that of simply stabilizing actin filaments has lead to a surge of reagents and techniques to study their function and mechanisms of action. This report is designed to provide a basic guide to the genes and proteins and the availability of reagents which allow effective study of this family of proteins. We highlight the value of combining multiple techniques to better evaluate the function of different tm isoforms and discuss the limitations of selected reagents. Brief background material is included to demystify some of the unfortunate complexity regarding this multi-gene family of proteins including the unconventional nomenclature of the isoforms and the evolutionary relationships of isoforms between species. Additionally, we present step-by-step detailed experimental protocols used in our laboratory to assist new comers to the field and experts alike.
