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Anti-Tropomyosin-4, clone 2D2A12, Cat. No. MABT1334, is a mouse monoclonal antibody that detects Tropomyosin delta-4 chain and is tested for use in Western Blotting.
More>>Anti-Tropomyosin-4, clone 2D2A12, Cat. No. MABT1334, is a mouse monoclonal antibody that detects Tropomyosin delta-4 chain and is tested for use in Western Blotting. Less<<
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Übersicht
Replacement Information
Description
Catalogue Number
MABT1334-25UL
Description
Anti-Tropomyosin-4 Antibody, clone 2D2A12
Alternate Names
Tropomyosin delta-4 chain
TM-4
Background Information
Tropomyosin alpha-4 chain (UniProt: P09495; also known as Tropomyosin-4, TM-4) is encoded by the Tpm4 gene (Gene ID: 24852) in rat. Tropomyosins are dimers of coiled-coil proteins that provide stability to actin filaments and regulate access of other actin-binding proteins. Tropomyosin-4 1 is a cytoskeletal protein that associates with F-actin stress fibers and binds to actin filaments in muscle and non-muscle cells. It plays a central role, in association with the troponin complex, in the calcium dependent regulation of vertebrate striated muscle contraction. It has a coiled coil structure formed by two polypeptide chains. It is abundantly expressed in kidney, lung, and spleen and is also observed in muscle and stomach tissues. Loss of Tpm4.1 is reported to lead to disturbance in cell-cell adhesions and invasive behavior in breast epithelial cells through Rac1-myosin IIB signaling. In colon cancer cells, TPM4 mRNA and protein expressions is shown to be significantly reduced and its low expression correlates with the clinal stage. Clone 2D2A12 detects both rat and human tropomyosin-4 and does not react with other exon1b containing isoforms from other tropomyosins. (Ref.: Schevzov, G., et al. (2011). BioArchitecture, 1 (4); 135-164; Jeong, S., et al. (2017). Oncotarget. 8(20); 33544-33559; Yang, R., et al. (2018). Biomed. Pharmacother. 101; 1-7).
References
Product Information
Format
Purified
Presentation
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Applications
Application
Anti-Tropomyosin-4, clone 2D2A12, Cat. No. MABT1334, is a mouse monoclonal antibody that detects Tropomyosin delta-4 chain and is tested for use in Western Blotting.
Key Applications
Western Blotting
Application Notes
Western Blotting Analysis: A representative lot detected Tropomyosin-4 in Western Blotting applications (Schevzov, G., et. al. (2011). Bioarchitecture. 1(4):135-164).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Biological Information
Immunogen
KLH-conjugated linear peptide corresponding to 19 amino acids from the N-terminal region of rat Tropomysin-4 corresponding to exon 1b in Tpm4 gene.
Clone
2D2A12
Concentration
0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Host
Mouse
Specificity
Clone 2D2A12 is a mouse monoclonal antibody that detects rat Tropomyosin-4. It targets an epitope within 19 amino acids from the N-terminal region corresponding to exon 1b in Tpm4 gene.
Isotype
IgG1κ
Species Reactivity
Human
Species Reactivity Note
Human. Predicted to react with Rat based on 100% sequence homology.
~30 kDa observed; 28.51 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance
Evaluated by Western Blotting in lysates from mouse skeletal muscle (Negative control) and human BJ fibroblasts (Positive control).
Western Blotting Analysis: A 1:500 dilution of this antibody detected Tropomyosin-4 in lysate from human BJ fibroblasts, but not in mouse skeletal muscle extract (Negative control).
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Tropomyosins are rod-like dimers which form head-to-tail polymers along the length of actin filaments and regulate the access of actin binding proteins to the filaments.1 The diversity of tropomyosin isoforms, over 40 in mammals, and their role in an increasing number of biological processes presents a challenge both to experienced researchers and those new to this field. The increased appreciation that the role of these isoforms expands beyond that of simply stabilizing actin filaments has lead to a surge of reagents and techniques to study their function and mechanisms of action. This report is designed to provide a basic guide to the genes and proteins and the availability of reagents which allow effective study of this family of proteins. We highlight the value of combining multiple techniques to better evaluate the function of different tm isoforms and discuss the limitations of selected reagents. Brief background material is included to demystify some of the unfortunate complexity regarding this multi-gene family of proteins including the unconventional nomenclature of the isoforms and the evolutionary relationships of isoforms between species. Additionally, we present step-by-step detailed experimental protocols used in our laboratory to assist new comers to the field and experts alike.
