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Anti-Tropomyosin 3, clone 2G10.2, Cat. No. MABT1335, is a mouse monoclonal antibody that detects Tropomyosin Tpm3.1 and Tpm3.2 and has been tested for use in Immunofluorescence and Western Blotting.
More>>Anti-Tropomyosin 3, clone 2G10.2, Cat. No. MABT1335, is a mouse monoclonal antibody that detects Tropomyosin Tpm3.1 and Tpm3.2 and has been tested for use in Immunofluorescence and Western Blotting. Less<<
Elix® 40 Hochleistungs-Wasseraufbereitungssystem 230 V 50/60 Hz (LC)
Übersicht
Replacement Information
Description
Catalogue Number
MABT1335-25UG
Description
Anti-Tropomyosin 3 Antibody, clone 2G10.2
Alternate Names
Gamma-tropomyosin
Tropomyosin-3
Tropomyosin-5
TPM3
Tm3
Background Information
Tropomyosin alpha-3 chain (UniProt: Q63610; also known as Gamma-tropomyosin, Tropomyosin-3, Tropomyosin-5) is encoded by the Tpm3 (also known as Tpm-5, Tpm5) gene (Gene ID: 117557) in rat. The TPM3 gene codes for the slow-twitch skeletal muscle isoform ( s Tm) and at least 9 LMW cytoskeletal isoforms referred to as Tm5NM1 to Tm5NM11. Tropomyosins are dimers of coiled-coil proteins that provide stability to actin filaments and regulate access of other actin-binding proteins. In muscle cells, they regulate muscle contraction by controlling the binding of myosin heads to the actin filament. In non-muscle cells tropomyosins are implicated in stabilizing cytoskeleton actin filaments. Tropomyosins have been implicated in the pathogenesis of cancer where high molecular weight isoforms are consistently down-regulated in transformed cells, while malignant cells display an increased reliance on low molecular weight isoforms. Tropomyosin 3 is a homodimeric protein that can form heterodimers with a beta (TPM2) chain. Its coiled coil structure is formed by 2 polypeptide chains. (Ref.: Glass, JJ et al. (2015). BMC Cancer. 15; 712).
References
Product Information
Format
Purified
Presentation
Purified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Applications
Application
Anti-Tropomyosin 3, clone 2G10.2, Cat. No. MABT1335, is a mouse monoclonal antibody that detects Tropomyosin Tpm3.1 and Tpm3.2 and has been tested for use in Immunofluorescence and Western Blotting.
Key Applications
Immunofluorescence
Western Blotting
Application Notes
Western Blotting Analysis: 1 µg/mL from a representative lot detected Tropomyosin 3 in human lung tissue lysate.
Immunofluorescence Analysis: A representative lot detected Tropomyosin 3 in Immunofluorescence applications (Schevzov, G., et. al. (2011). Bioarchitecture. 1(4):135-164).
Western Blotting Analysis: A representative lot detected Tropomyosin 3 in Western Blotting applications (Schevzov, G., et. al. (2011). Bioarchitecture. 1(4):135-164).
Biological Information
Immunogen
Diphtheria Toxoid Carrier Protein-conjugated linear peptide corresponding to 27 amino acids from the C-terminal region of rat Tpm3.1 and Tpm3.2.
Epitope
C-Terminus of Tpm 3.1 and Tpm 3.2
Clone
2G10.2
Concentration
Please refer to lot specific datasheet.
Host
Mouse
Specificity
Clone 2G10.2 is a mouse monoclonal antibody that specifically detects Tpm3.1 and Tpm3.2. It targets an epitope within 27 amino acids from the C-terminal region.
~30 kDa observed; 29.00 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance
Evaluated by Western Blotting in mouse liver tissue lysate.
Western Blotting Analysis: 1 µg/mL of this antibody detected Tropomyosin 3 in mouse liver tissue lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Tropomyosins are rod-like dimers which form head-to-tail polymers along the length of actin filaments and regulate the access of actin binding proteins to the filaments.1 The diversity of tropomyosin isoforms, over 40 in mammals, and their role in an increasing number of biological processes presents a challenge both to experienced researchers and those new to this field. The increased appreciation that the role of these isoforms expands beyond that of simply stabilizing actin filaments has lead to a surge of reagents and techniques to study their function and mechanisms of action. This report is designed to provide a basic guide to the genes and proteins and the availability of reagents which allow effective study of this family of proteins. We highlight the value of combining multiple techniques to better evaluate the function of different tm isoforms and discuss the limitations of selected reagents. Brief background material is included to demystify some of the unfortunate complexity regarding this multi-gene family of proteins including the unconventional nomenclature of the isoforms and the evolutionary relationships of isoforms between species. Additionally, we present step-by-step detailed experimental protocols used in our laboratory to assist new comers to the field and experts alike.
