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09-126
Sigma-AldrichAnti-Tribbles-1 Antibody
Anti-Tribbles-1, Cat. No. 09-126, is a rabbit polyclonal antibody that detects Tribbles-1 and is tested for use in Immunohistochemistry (Paraffin), Peptide Inhibition Assay, and Western Blotting.
More>>Anti-Tribbles-1, Cat. No. 09-126, is a rabbit polyclonal antibody that detects Tribbles-1 and is tested for use in Immunohistochemistry (Paraffin), Peptide Inhibition Assay, and Western Blotting. Less<<
Anti-Tribbles-1 Antibody: SDB (Sicherheitsdatenblätter), Analysenzertifikate und Qualitätszertifikate, Dossiers, Broschüren und andere verfügbare Dokumente.
Tribbles homolog 1 (UniProt: Q96RU8; also known as TRB-1, G-protein-coupled receptor-induced gene 2 protein, GIG-2, SKIP1) is encoded by the TRIB1 (also known as C8FW, GIG2, TRB1) gene (Gene ID: 10221) in human. TRB-1 is an evolutionary conserved monomeric protein that acts as a pseudokinase. It is widely distributed, but highest concentrations are found in the bone marrow and the thyroid gland. In mammals, it controls several interacting signaling pathways, including MAP kinases and PI-3 kinase pathways. In the N-terminal region it contains a PEST region and a pseudokinase domain. In the C-terminal region it has a COP1-binding region (aa 355-360). Due to the lack of a functional ATP-binding site it is catalytically inactive but serves as an adapter or scaffold that facilitates assembly of other proteins. Its COP1-binding motif is masked by autoinhibitory interactions with the protein kinase domain. TRB-1 has been established as an oncogene in acute myeloid leukemia and his reported to play a pivotal role in the differentiation of anti-inflammatory M2-macrophages in the pathogenesis of early atherosclerosis and during adipose tissue inflammation. It is also shown to be overexpressed in prostate cancer and is associated with cancer risk. (Ref.: Niespolo, C.., et a. (2020). Front. Immunol. 11; 574046; Kiss-Toth, E., et al. (2004). J. Biol. Chem. 279(41); 42703-42708).
References
Product Information
Format
Affinity Purified
Control
HeLa
Presentation
Purified rabbit polyclonal antibody in PBS with 0.05% sodium azide.
Anti-Tribbles-1, Cat. No. 09-126, is a rabbit polyclonal antibody that detects Tribbles-1 and is tested for use in Immunohistochemistry (Paraffin), Peptide Inhibition Assay, and Western Blotting.
Key Applications
Western Blotting
Peptide Inhibition Assay
Immunohistochemistry (Paraffin)
Application Notes
Tested applications
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Tribbles-1 in Human Skeletal Muscle tissue lysate.
Immunohistochemistry (Paraffin) Analysis: A 1:250 Dilution from a representative lot detected Tribbles-1 in human atherosclerotic aorta tissue sections.
Peptide Inhibition Assay: Target band detection in lysate of Human skeletal muscle tissue was prevented by preblocking of a representative lot with the immunogen peptide.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Biological Information
Immunogen
KLH-conjugated linear peptide corresponding to 14 amino acids from the N-terminal region of human Tribbles homolog 1 (TRB-1).
Epitope
N-terminal
Host
Rabbit
Specificity
This rabbit polyclonal antibody specifically detects human Tribbles-1 (TRB-1). It targets an epitope within 14 amino acids from the N-terminal region.
Target molecular weight ~47 kDa observed. 41.01 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance
Evaluated by Western Blotting in acid extract of HeLa cells.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Tribbles-1 in Acid extract of HeLa cells.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Store at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Development of the atherosclerotic plaque involves a complex interplay between a number of cell types and an extensive inter-cellular communication via cell bound as well as soluble mediators. The family of tribbles proteins has recently been identified as novel controllers of pro-inflammatory signal transduction. The objective of this study was to address the expression pattern of all three tribbles proteins in atherosclerotic plaques from a mouse model of atherosclerosis. Each tribbles were expressed in vascular smooth muscle cells, endothelial cells as well as in resident macrophages of mouse atherosclerotic plaques. The role of IL-1 mediated inflammatory events in controlling tribbles expression was also addressed by inducing experimental atherosclerosis in ApoE-/-IL1R1-/- (double knockout) mice. Immunohistochemical analysis of these mice showed a selective decrease in the percentage of trb-1 expressing macrophages, compared to the ApoE-/- cohort (14.7% ± 1.55 vs. 26.3% ± 1.19). The biological significance of this finding was verified in vitro where overexpression of trb-1 in macrophages led to a significant attenuation (~70%) of IL-6 production as well as a suppressed IL-12 expression induced by a proinflammatory stimulus. In this in vitro setting, expression of truncated trb-1 mutants suggests that the kinase domain of this protein is sufficient to exert this inhibitory action.
