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MABE1084 Anti-Topoisomerase I-DNA Covalent Complexes Antibody, clone 1.1A

Anti-Topoisomerase I-DNA Covalent Complexes Antibody, clone 1.1A is an antibody against Topoisomerase I-DNA Covalent Complexes for use in Immunocytochemistry, Flow Cytometry, Dot Blot, ELISA.

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Research Category: Epigenetics & Nuclear Function Research Sub-Category: Chromatin Biology Gene Symbol: TOP1, TOPI UniProt Number: P11387
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MABE1084
100 μg  
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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, MDB, ELISA, FC, ICCMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABE1084
      DescriptionAnti-Topoisomerase I-DNA Covalent Complexes Antibody, clone 1.1A
      Alternate Names
      • Topoisomerase I-DNA covalent complex
      • DNA topoisomerase 1-DNA covalent complex
      • Topo I-DNA covalent complex
      • TopoI cc
      • TopoIcc
      Background InformationDNA topoisomerase 1 (EC 5.99.1.2; UniProt P11387; also known as DNA topoisomerase I, Topo I) is encoded by the TOP1 (also known as TOPI) gene (Gene ID 7150) in human. Topo I relaxes double strand DNA (dsDNA) torsional strain by nicking one strand of the dsDNA to allow rotation of the nicked 3'-hydroxy end around the unnicked strand, while the 5′-phosphoryl end of the nicked DNA forms a covalent phosphodiester bond with a tyrosine residue on Topo I. The nicked 3'-hydroxy end then attacks the DNA-enzyme phosphoester bond to religate the nicked DNA strand. One supercoil is removed during each round of Topo I-catalyzed nicking and resealing. Camptothecins are widely used for cancer treatment, these drugs intercalate into DNA at the topo I active site, inhibiting the religation step of the enzyme and shifting the equilibrium toward covalent topo I-DNA complexes rather than free topo I and DNA. Interaction of these drug-stabilized covalent topo I-DNA complexes with advancing replication forks or transcription complexes causse further DNA damage and eventual cell death.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Topoisomerase I-DNA Covalent Complexes Antibody, clone 1.1A is an antibody against Topoisomerase I-DNA Covalent Complexes for use in Immunocytochemistry, Flow Cytometry, Dot Blot, ELISA.
      Key Applications
      • Dot Blot
      • ELISA
      • Flow Cytometry
      • Immunocytochemistry
      Application NotesFlow Cytometry Analysis: 1.0 µg of this antibody from a representative lot detected topoisomerase I/DNA covalent complexes in Topotecan-treated HCT116 human colon cancer cells.

      Immunocytochemistry Analysis: 10 µg/mL from a representative lot detected topoisomerase I/DNA covalent complexes in Topotecan-treated A549 human lung carcinoma cells (Courtesy of Dr. Scott H. Kaufmann, Mayo Clinic, Rochester, MN).

      Flow Cytometry Analysis: 10 µg/mL from a representative lot detected topoisomerase I/DNA covalent complexes in Topotecan-treated HCT116 human colon cancer cells (Courtesy of Dr. Scott H. Kaufmann, Mayo Clinic, Rochester, MN).

      Dot Blot Analysis: A representative lot detected the Topotecan-/TPT-stablized topoisomerase I/DNA covalent complexes, but not free topoisomerase I (non-DNA complexed) by slot blot using CsCl2 gradient-fractionated lysates from TPT-treated and untreated A549 cells (Patel, A.G., et al, (2016). 44(6):2816-2826).

      Dot Blot Analysis: A representative lot detected a Topotecan (TPT) dose-dependent increase of TPT-stabilized topoisomerase I/DNA covalent complexes in lysates from TPT-treated A549 and HCT116 cells by slot blot (Patel, A.G., et al, (2016). 44(6):2816-2826).

      Dot Blot Analysis: A representative lot detected stabilized topoisomerase I/DNA covalent complexes in lysates from A549 cells treated with Camptothecins (CPT, SN-38, TPT) or Indenoisoquinolines (NSC 314622, NSC 725776, NSC 743400), but not nucleoside analogues (cytarabine and gemcitabine) (Patel, A.G., et al, (2016). 44(6):2816-2826).

      ELISA Analysis: A representative lot detected the immunogen peptide corresponding to topoisomerase I active site sequence with phosphorylated Tyr723, but not the peptide with non-phosphorylated Tyr273 by direct (non-sandwich) ELISA (Patel, A.G., et al, (2016). 44(6):2816-2826).

      Immunocytochemistry Analysis: A representative lot detected a dose-dependent increase of non-nucleolar topoisomerase I/DNA covalent complex loci in Topotecan-/TPT-treated A549 cells, while the small number of loci in untreated cells were seen only in nucleoli (Patel, A.G., et al, (2016). 44(6):2816-2826).

      Immunocytochemistry Analysis: A representative lot detected a temporally and spatially distinct nuclear loci formation of topoisomerase I/DNA covalent complexes from those of phospho-H2AX and Rad51 in Topotecan-/TPT-treated A549 cells (Patel, A.G., et al, (2016). 44(6):2816-2826).
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to human Topo I target site sequence with phosphorylated Tyr723.
      EpitopeTopo I pTyr723.
      Clone1.1A
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone 1.1A specifically recognizes topoisomerase I (Topo I) in DNA covalent complexes, but not non-DNA complexed, free Topo I by detecting topo I active site Tyr723 phosphorylation (Patel, A.G., et al, (2016). 44(6):2816-2826).
      IsotypeIgG2bκ
      Species Reactivity
      • Human
      • Mouse
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • TOP1
      • TOPI
      Purification MethodProtein G purified.
      UniProt Number
      Molecular Weight90.7 kDa calculated.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunocytochemistry in Topotecan-treated A549 human lung carcinoma cells.

      Immunocytochemistry Analysis: 10 µg/mL of this antibody detected topoisomerase I/DNA covalent complexes in Topotecan-treated A549 human lung carcinoma cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Bestellnummer GTIN
      MABE1084 04055977314267

      Documentation

      Anti-Topoisomerase I-DNA Covalent Complexes Antibody, clone 1.1A SDB

      Titel

      Sicherheitsdatenblatt (SDB) 

      Anti-Topoisomerase I-DNA Covalent Complexes Antibody, clone 1.1A Analysenzertifikate

      TitelChargennummer
      Anti-Topoisomerase I-DNA Covalent Complexes, clone 1.1A - 2943572 2943572
      Anti-Topoisomerase I-DNA Covalent Complexes, clone 1.1A - 3176723 3176723
      Anti-Topoisomerase I-DNA Covalent Complexes, clone 1.1A - 3257002 3257002
      Anti-Topoisomerase I-DNA Covalent Complexes, clone 1.1A - 3484975 3484975
      Anti-Topoisomerase I-DNA Covalent Complexes, clone 1.1A - 3536096 3536096
      Anti-Topoisomerase I-DNA Covalent Complexes, clone 1.1A - 3616475 3616475
      Anti-Topoisomerase I-DNA Covalent Complexes, clone 1.1A - 3731563 3731563
      Anti-Topoisomerase I-DNA Covalent Complexes, clone 1.1A - 3890507 3890507
      Anti-Topoisomerase I-DNA Covalent Complexes, clone 1.1A - 3923048 3923048
      Anti-Topoisomerase I-DNA Covalent Complexes, clone 1.1A - 4014581 4014581
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