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MABC1628-100UG Anti-TNFSF15 Antibody, clone Tan 2-2

MABC1628-100UG
100 μg  
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      Description
      Catalogue NumberMABC1628-100UG
      DescriptionAnti-TNFSF15 Antibody, clone Tan 2-2
      Alternate Names
      • Tumor necrosis factor ligand superfamily member 15
      • TNF ligand-related molecule 1
      • Vascular endothelial cell growth inhibitor
      Background InformationTumor necrosis factor ligand superfamily member 15 (UniProt: Q5UBV8; also known as TNF ligand-related molecule 1, TL1A, Vascular endothelial cell growth inhibitor) is encoded by the Tnfsf15 (also known as Tl1, Vegi) gene (Gene ID: 326623) in murine species. TNFSF15 is a single-pass type II membrane protein of the tumor necrosis factor family that is expressed on activated macrophages, dendritic cells, T cells, and endothelial cells and is produced either as a membrane-anchored protein or a soluble cytokine. TNFSF15 has a cytoplasmic domain (aa 1-39), a transmembrane domain (40-60), and an extracellular domain (aa 61-252). Its expression is increased in macrophages and dendritic cells in response to various stimuli. It serves as a ligand for death receptor 3 (DR3) and mediates the activation of NF-kappa-B. It is a key player in the regulation of T-cell-mediated immune responses and promotes T cell immunity to pathogens and directly co-stimulates group 2 and 3 innate lymphoid cells. It is also shown to enhance the proliferation of in vitro generated Th17 cells but does not affect the proliferation of Th1 cells. TNFSF15 can also directly osteoclastogenesis in the presence of RANK-L and M-CSF in a DR3-dependent manner. (Ref.: Ferdinand, JR., et al. (2018). J. Immunol. 200: 1360 1369.Buchan, SL., et al (2012). Eur. J. Immunol. 42; 580 588; Bull, MJ., et al. (2008). J. Exp. Med. Vol. 205 No. 11 2457-2464).
      Product Information
      FormatPurified
      PresentationPurified rat monoclonal antibody IgG2a in PBS without preservatives.
      Quality LevelMQ200
      Applications
      ApplicationAnti-TNFSF15, clone Tan 2-2, Cat. No. MABC1628, is a rat monoclonal antibody that detects TNFSF15 and is tested for use in Flow Cytometry, Immunofluorescence, Immunohistochemistry, Neutralizing, and Western Blotting.
      Key Applications
      • Flow Cytometry
      • Immunofluorescence
      • Immunohistochemistry
      • Neutralizing
      • Western Blotting
      Application NotesWestern Blotting Analysis: A 1:1,000 dilution from a representative lot detected TNFSF15 in Mouse lung tissue lysate.

      Flow Cytometry Analysis: A representative lot detected TNFSF15 in Flow Cytometry applications (Slebioda, T.J., et. al. (2011). Eur J Immunol. 41(9):2606-11; Bull, M.J., et. al. (2008). J Exp Med. 205(11):2457-64; Ferdinand, J.R., et. al. (2018). J Immunol. 200(4):1360-1369).

      Immunohistochemistry Applications: A representative lot detected TNFSF15 in Immunohistochemistry applications (Richard, A.C., et. al. (2015). J Immunol. 195(12):5839-40).

      Immunofluorescence Analysis: A representative lot detected TNFSF15 in Immunofluorescence applications (Taraban, V.Y., et. al. (2011). Mucosal Immunol. 4(2):186-96; Buchan, S.L., et. al. (2012). Eur J Immunol. 42(3):580-8).

      Neutralizing: A representative lot protected animals in a systemic model of Rheumatoid arthritis disease. (Bull, M.J., et. al. (2008). J Exp Med. 205(11):2457-64; Slebioda, T.J., et. al. (2011). Eur J Immunol. 41(9):2606-11).

      Multiplexing: A representative lot detected TNFSF15 in Multiplexing applications (Ferdinand, J.R., et. al. (2018). J Immunol. 200(4):1360-1369).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
      Biological Information
      ImmunogenRecombinant fragment corresponding to 176 amino acids from the extracellular domain of murine TNFSF15 TL1A protein with a human IgG1 Fc domain and an additional hinge-like region at the C-terminus.
      Epitopeextracellular domain
      CloneTan 2-2
      Concentration1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostRat
      SpecificityClone Tan2-2 is a rat monoclonal antibody that detects murine Tumor necrosis factor ligand superfamily member 15 (TNFSF15). It targets an epitope within the extracellular domain.
      IsotypeIgG2aκ
      Species Reactivity
      • Mouse
      Species Reactivity NoteMouse.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Tnfsf15
      • Tl1
      • Vegi
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~25 kDa observed; 27.72 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in Mouse kidney tissue lysate.

      Western Blotting Analysis: 1 µg/mL of this antibody detected TNFSF15 in Mouse kidney tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -10°C to -25°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 μg
      Global Trade Item Number
      Bestellnummer GTIN
      MABC1628-100UG 04061842592067