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MABS1719 Anti-TMEM64 Antibody, clone 7A2.1

Detect TMEM64 using this mouse monoclonal Anti-TMEM64, clone 7A2.1 Antibody, Cat. No. MABS1719, validated for use in Western Blotting.

Anti-TMEM64 Antibody, clone 7A2.1: SDB (Sicherheitsdatenblätter), Analysenzertifikate und Qualitätszertifikate, Dossiers, Broschüren und andere verfügbare Dokumente.
Research Category: Signaling Gene Symbol: TMEM64 UniProt Number: Q6YI46
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MABS1719
100 μg  
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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWBMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABS1719
      DescriptionAnti-TMEM64 Antibody, clone 7A2.1
      Alternate Names
      • Transmembrane protein 64
      Background InformationTransmembrane protein 64 (UniProt Q6YI46) is encoded by the TMEM64 gene (Gene ID 169200) in human. TMEM64 is a 6-transmembrane protein that plays a role in modulating Wnt/ -catenin signaling-mediated osteoblast and adipocyte differentiation. TMEM64 interacts and positively regulates sarcoplasmic endoplasmic reticulum Ca(2+) ATPase 2 (SERCA2) activity, which is important for RANKL-induced Ca(2+) oscillation, Ca(2+)/calmodulin-dependent protein kinases (CaMK) IV activity and mitochondrial ROS formation needed for for osteoclast formation. Mice with Tmem64 deficiency are characterized by a high-bone-mass phenotype, displaying higher levels of ALP, osteocalcin (Ocn), and type 1 collagen (Col 1a) transcripts than in wild-type mice. Tmem64-knockout in mice is reported to markedly enhance osteoblast differentiation and inhibit adipocyte differentiation from bone marrow-derived stromal cells (BMSCs), resulting in a preferential switch of mesenchymal progenitors favoring the osteogenic over the adipogenic lineage.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationDetect TMEM64 using this mouse monoclonal Anti-TMEM64, clone 7A2.1 Antibody, Cat. No. MABS1719, validated for use in Western Blotting.
      Key Applications
      • Western Blotting
      Application NotesWestern Blotting Analysis: 2 µg/mL of this antibody detected TMEM64 in 10 µg of SK-MEL-28 cell lysate.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to the C-terminal end sequence of human and rat TMEM64.
      Clone7A2.1
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityThe immunogen sequence is 100% conserved between human and rat species and present in all four human spliced isoforms reported by UniProt (Q6YI46 & D3ZV19). Murine cross-reactivity is highly likely, but has not been confirmed.
      IsotypeIgG2aκ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman. Predicted to react with Rat based on 100% sequence homology.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • TMEM64
      Purification MethodProtein G purified.
      UniProt Number
      Molecular Weight~45 kDa observed. 39.67/12.95/14.25/33.97 kDa (human isoform 1/2/3/4) and 39.53 kDa (rat) calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in A431 cell lysate.

      Western Blotting Analysis: 2 µg/mL of this antibody detected TMEM64 in 10 µg of A431 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Bestellnummer GTIN
      MABS1719 04054839081422