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AB10545 Anti-TIGAR Antibody

Anti-TIGAR Antibody is an antibody against TIGAR for use in WB, IH(P).

Anti-TIGAR Antibody: SDB (Sicherheitsdatenblätter), Analysenzertifikate und Qualitätszertifikate, Dossiers, Broschüren und andere verfügbare Dokumente.
Research Category: Apoptosis & Cancer Research Sub-Category: Apoptosis - Additional Gene Symbol: TIGAR UniProt Number: Q9NQ88
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AB10545
100 µg  
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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, R, BWB, IH(P)RbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberAB10545
      DescriptionAnti-TIGAR Antibody
      Alternate Names
      • chromosome 12 open reading frame 5
      • TP53-induced glycolysis and apoptosis regulator
      • transactivated by NS3TP2 protein
      • probable fructose-2,6-bisphosphatase TIGAR
      Background InformationTIGAR (TP53-induced glycolysis and apoptosis regulator) is a p53-inducible protein that is thought to diminish levels of reactive oxygen species (ROS) and this may also contribute to p53 protecting from genomic damage. TIGAR’s ability to lower ROS levels may also have an important role to play in the autophagic response. It is known to decrease levels of fructose-2,6-bisphosphate and in turn inhibits glycolysis. Under ischemic stress, recent research has shown that TIGAR and p53 can regulate cellular energy homeostasis and apoptosis.
      References
      Product Information
      FormatAffinity Purified
      Control
      • HeLa cell lysate
      PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-TIGAR Antibody is an antibody against TIGAR for use in WB, IH(P).
      Key Applications
      • Western Blotting
      • Immunohistochemistry (Paraffin)
      Application NotesImmunohistochemistry Analysis: A 1:400 dilution from a previous lot detected TIGAR in normal human brain and glioblastoma cells.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to human TIGAR.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      Species Reactivity
      • Human
      • Mouse
      • Rat
      • Bovine
      Species Reactivity NoteDemonstrated to react with human, mouse, and rat.
      Predicted to react with bovine based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene is regulated as part of the p53 tumor suppressor pathway and encodes a protein with sequence similarity to the bisphosphate domain of the glycolytic enzyme that degrades fructose-2,6-bisphosphate. The protein functions by blocking glycolysis and directing the pathway into the pentose phosphate shunt. Expression of this protein also protects cells from DNA damaging reactive oxygen species and provides some protection from DNA damage-induced apoptosis. The 12p13.32 region that includes this gene is paralogous to the 11q13.3 region. [provided by RefSeq].
      Gene Symbol
      • TIGAR
      Purification MethodAffinity Purfied
      UniProt Number
      UniProt SummaryFUNCTION: Probable fructose-biphosphatase. Lowers cellular levels of fructose 2,6-bisphosphate. Protects cells against reactive oxygen species and against apoptosis induced by TP53/p53.

      CATALYTIC ACTIVITY: Beta-D-fructose 2,6-bisphosphate + H2O = D-fructose 6-phosphate + phosphate.

      INDUCTION: Rapidly up-regulated by TP53/p53.

      PTM: Phosphorylated upon DNA damage, probably by ATM or ATR.

      SEQUENCE SIMILARITIES: Belongs to the phosphoglycerate mutase family.

      CAUTION: Not expected to have any kinase activity.
      Molecular Weight~30 kDa observed
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in HeLa cell lysate.

      Western Blot Analysis: 0.5 µg/mL of this antibody detected TIGAR in 10 µg of HeLa cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Bestellnummer GTIN
      AB10545 04053252292040