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GF10 Anti-TGF-α (Ab-2) Mouse mAb (213-4.4)

This Anti-TGF-α (Ab-2) Mouse mAb (213-4.4) is validated for use in Frozen Sections, Immunocytochemistry, Immunoblotting Paraffin Sections for the detection of TGF-α (Ab-2).

Anti-TGF-α (Ab-2) Mouse mAb (213-4.4): SDB (Sicherheitsdatenblätter), Analysenzertifikate und Qualitätszertifikate, Dossiers, Broschüren und andere verfügbare Dokumente.

Synonyme: Anti-Transforming Growth Factor-α

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      Übersicht

      Replacement Information

      Key Spec Table

      Species ReactivityHostAntibody Type
      Ca, Ht, H, M, RMMonoclonal Antibody
      Description
      OverviewRecognizes the ~6 kDa TGF-α protein in A2058 cells or skin tissue.
      Catalogue NumberGF10
      Brand Family Calbiochem®
      SynonymsAnti-Transforming Growth Factor-α
      References
      ReferencesCoffey, R.J., et al. 1987. Nature 328, 817.
      Derynck, R. 1986. J. Cell. Biochem. 32, 203.
      Samsoondor, J., et al. 1986. J. Biol. Chem. 261, 14408.
      Lee, D.C., et al. 1985. Molec. Cell. Biol. 5, 3644.
      Kimball, E.S., et al. 1984. Cancer Res. 44, 3613.
      Marquardt, H., et al. 1983. Proc. Natl. Acad. Sci. USA 80, 4684.
      Reynolds, F.H., Jr., et al. 1983. Nature 292, 259.
      Todaro, G.J., et al. 1983. In Genes and Proteins in Oncogenesis, Vogel, H. and Weinstein, I.B. (Eds.) New York, Raven Press p. 165.
      Product Information
      FormLiquid
      FormulationIn 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.5.
      Positive controlA2058 cells or skin tissue
      Preservative≤0.1% sodium azide
      Quality LevelMQ100
      Applications
      Application ReferencesOriginal Clone Sorvillo, J.M., et al. 1990. Oncogene 5, 377.
      Key Applications Frozen Sections
      Immunocytochemistry
      Not Western Blot
      Paraffin Sections
      Application NotesFrozen Sections (1-5 µg/ml)
      Immunocytochemistry (1-5 µg/ml)
      Immunoblotting (not recommended)
      Paraffin Sections (1-10 µg/ml, saponin pre-treatment required)
      Application CommentsDoes not cross-react with human or mouse EGF. Antibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogenrecombinant human TGF-α (~6 kDa mature form)
      ImmunogenHuman
      Clone213-4.4
      HostMouse
      IsotypeIgG2a
      Species Reactivity
      • Canine
      • Hamster
      • Human
      • Mouse
      • Rat
      Antibody TypeMonoclonal Antibody
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Do not freeze Yes
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Bestellnummer GTIN
      GF10 0

      Documentation

      Anti-TGF-α (Ab-2) Mouse mAb (213-4.4) SDB

      Titel

      Sicherheitsdatenblatt (SDB) 

      Anti-TGF-α (Ab-2) Mouse mAb (213-4.4) Analysenzertifikate

      TitelChargennummer
      GF10

      Literatur

      Übersicht
      Coffey, R.J., et al. 1987. Nature 328, 817.
      Derynck, R. 1986. J. Cell. Biochem. 32, 203.
      Samsoondor, J., et al. 1986. J. Biol. Chem. 261, 14408.
      Lee, D.C., et al. 1985. Molec. Cell. Biol. 5, 3644.
      Kimball, E.S., et al. 1984. Cancer Res. 44, 3613.
      Marquardt, H., et al. 1983. Proc. Natl. Acad. Sci. USA 80, 4684.
      Reynolds, F.H., Jr., et al. 1983. Nature 292, 259.
      Todaro, G.J., et al. 1983. In Genes and Proteins in Oncogenesis, Vogel, H. and Weinstein, I.B. (Eds.) New York, Raven Press p. 165.

      Literaturstellen

      Titel
    • Mark R. Bush, et al. (1998) Evidence of Juxtacrine Signaling for Transforming Growth Factor a in Human Endometrium. 59, 1522-1529.
      		•
      Datenblatt

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision23-August-2007 RFH
      SynonymsAnti-Transforming Growth Factor-α
      ApplicationFrozen Sections (1-5 µg/ml)
      Immunocytochemistry (1-5 µg/ml)
      Immunoblotting (not recommended)
      Paraffin Sections (1-10 µg/ml, saponin pre-treatment required)
      DescriptionPurified mouse monoclonal antibody generated by immunizing Balb/c mice with the specified immunogen and fusing splenocytes with P3 x 63 Ag 8.653 mouse myeloma cells (see application references). Recognizes the ~6 kDa TGF-α protein.
      BackgroundTransforming growth factor-alpha (TGF-α) is an acid- and heat-stable 50 amino acid protein of 5500 MW originally found in rodents and humans. TGF-α is 33% homologous at the amino acid level to epidermal growth factor (EGF). TGF-α binds to the EGF receptor, mediates tyrosine phosphorylation of the receptor, and promotes anchorage-independent growth of normal rat fibroblasts in soft agar in the presence of transforming growth factor-beta. TGF-α is secreted by a variety of transformed cells and tumors, embryonic cells, and some normal adult cells. TGF-α bioactivity has been found in the urine of cancer patients. It has been suggested that it may act as an autocrine growth factor for the induction or maintenance of malignancy.
      HostMouse
      Immunogen speciesHuman
      Immunogenrecombinant human TGF-α (~6 kDa mature form)
      Clone213-4.4
      IsotypeIgG2a
      Speciescanine, hamster, human, mouse, rat
      Positive controlA2058 cells or skin tissue
      FormLiquid
      FormulationIn 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.5.
      Concentration Label Please refer to vial label for lot-specific concentration
      Preservative≤0.1% sodium azide
      CommentsDoes not cross-react with human or mouse EGF. Antibody should be titrated for optimal results in individual systems.
      Storage +2°C to +8°C
      Do Not Freeze Yes
      Toxicity Standard Handling
      ReferencesCoffey, R.J., et al. 1987. Nature 328, 817.
      Derynck, R. 1986. J. Cell. Biochem. 32, 203.
      Samsoondor, J., et al. 1986. J. Biol. Chem. 261, 14408.
      Lee, D.C., et al. 1985. Molec. Cell. Biol. 5, 3644.
      Kimball, E.S., et al. 1984. Cancer Res. 44, 3613.
      Marquardt, H., et al. 1983. Proc. Natl. Acad. Sci. USA 80, 4684.
      Reynolds, F.H., Jr., et al. 1983. Nature 292, 259.
      Todaro, G.J., et al. 1983. In Genes and Proteins in Oncogenesis, Vogel, H. and Weinstein, I.B. (Eds.) New York, Raven Press p. 165.
      Citation
    • Mark R. Bush, et al. (1998) Evidence of Juxtacrine Signaling for Transforming Growth Factor a in Human Endometrium. 59, 1522-1529.
      		•
      Application referencesOriginal Clone Sorvillo, J.M., et al. 1990. Oncogene 5, 377.

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