Wenn Sie das Fenster schließen, wird Ihre Konfiguration nicht gespeichert, es sei denn, Sie haben Ihren Artikel in die Bestellung aufgenommen oder zu Ihren Favoriten hinzugefügt.
Klicken Sie auf OK, um das MILLIPLEX® MAP-Tool zu schließen oder auf Abbrechen, um zu Ihrer Auswahl zurückzukehren.
Wählen Sie konfigurierbare Panels & Premixed-Kits - ODER - Kits für die zelluläre Signaltransduktion & MAPmates™
Konfigurieren Sie Ihre MILLIPLEX® MAP-Kits und lassen sich den Preis anzeigen.
Konfigurierbare Panels & Premixed-Kits
Unser breites Angebot enthält Multiplex-Panels, für die Sie die Analyten auswählen können, die am besten für Ihre Anwendung geeignet sind. Unter einem separaten Register können Sie das Premixed-Cytokin-Format oder ein Singleplex-Kit wählen.
Kits für die zelluläre Signaltransduktion & MAPmates™
Wählen Sie gebrauchsfertige Kits zur Erforschung gesamter Signalwege oder Prozesse. Oder konfigurieren Sie Ihre eigenen Kits mit Singleplex MAPmates™.
Die folgenden MAPmates™ sollten nicht zusammen analysiert werden: -MAPmates™, die einen unterschiedlichen Assaypuffer erfordern. -Phosphospezifische und MAPmate™ Gesamtkombinationen wie Gesamt-GSK3β und Gesamt-GSK3β (Ser 9). -PanTyr und locusspezifische MAPmates™, z.B. Phospho-EGF-Rezeptor und Phospho-STAT1 (Tyr701). -Mehr als 1 Phospho-MAPmate™ für ein einziges Target (Akt, STAT3). -GAPDH und β-Tubulin können nicht mit Kits oder MAPmates™, die panTyr enthalten, analysiert werden.
.
Bestellnummer
Bestellinformationen
St./Pkg.
Liste
Dieser Artikel wurde zu Ihren Favoriten hinzugefügt.
Wählen Sie bitte Spezies, Panelart, Kit oder Probenart
Um Ihr MILLIPLEX® MAP-Kit zu konfigurieren, wählen Sie zunächst eine Spezies, eine Panelart und/oder ein Kit.
Custom Premix Selecting "Custom Premix" option means that all of the beads you have chosen will be premixed in manufacturing before the kit is sent to you.
Catalogue Number
Ordering Description
Qty/Pack
List
Dieser Artikel wurde zu Ihren Favoriten hinzugefügt.
Spezies
Panelart
Gewähltes Kit
Menge
Bestellnummer
Bestellinformationen
St./Pkg.
Listenpreis
96-Well Plate
Menge
Bestellnummer
Bestellinformationen
St./Pkg.
Listenpreis
Weitere Reagenzien hinzufügen (MAPmates erfordern die Verwendung eines Puffer- und Detektionskits)
Menge
Bestellnummer
Bestellinformationen
St./Pkg.
Listenpreis
48-602MAG
Buffer Detection Kit for Magnetic Beads
1 Kit
Platzsparende Option Kunden, die mehrere Kits kaufen, können ihre Multiplex-Assaykomponenten in Kunststoffbeuteln anstelle von Packungen erhalten, um eine kompaktere Lagerung zu ermöglichen.
Dieser Artikel wurde zu Ihren Favoriten hinzugefügt.
Das Produkt wurde in Ihre Bestellung aufgenommen
Sie können nun ein weiteres Kit konfigurieren, ein Premixed-Kit wählen, zur Kasse gehen oder das Bestell-Tool schließen.
Anti-TCF-3/4, clone 6F12, Cat. No. MABE1993, is a mouse monoclonal antibody that detects Transcription factor 3-/4 and is tested for use in Immunocytochemistry, and Immunohistochemistry (Paraffin), and Western Blotting.
More>>Anti-TCF-3/4, clone 6F12, Cat. No. MABE1993, is a mouse monoclonal antibody that detects Transcription factor 3-/4 and is tested for use in Immunocytochemistry, and Immunohistochemistry (Paraffin), and Western Blotting. Less<<
Empfohlene Produkte
Übersicht
Replacement Information
Description
Catalogue Number
MABE1993-100UL
Description
Anti-TCF-3/4 Antibody, clone 6F12
Alternate Names
Transcription factor 4
Class B basic helix-loop-helix protein 19
bHLHb19
Immunoglobulin transcription factor 2
ITF-2
SL3-3 enhancer factor 2
SEF-2)
Background Information
Transcription factor 4 (UniProt: P15884; also known as TCF-4, Class B basic helix-loop-helix protein 19, bHLHb19, Immunoglobulin transcription factor 2, ITF-2, SL3-3 enhancer factor 2, SEF-2) is encoded by the TCF4 (also known as BHLHB19, ITF2, SEF2) gene (Gene ID: 6925) in human. TCF-4 is a member of the basic helix loop helix (bHLH) family of transcription factors that preferentially binds to either 5'-ACANNTGT-3' or 5'-CCANNTGG-3'. It is expressed in adult heart, brain, placenta, skeletal muscle and to a lesser extent in the lung. In developing embryonic tissues, its expression is mostly in the brain. TCF-4 plays an important role in several developmental processes, including the initiation of neuronal differentiation. The bHLH domain of TCF-4 is localized in amino acids 564-617 and contains a highly conserved group of positively charged amino acids that make up the basis region that interacts with DNA. The remaining part of bHLH forms a surface that favors its homo- and heterodimerization through the assembly of a stable four-helix bundle with a hydrophobic core. The bHLH domain permits TCF-4 to interact with several transcription factors. Its DNA-binding properties are shown to be regulated by Calcium binding proteins, such as calmodulin and S100 alpha and beta. Clone 6F12 is shown to react with both TCF-3 and TCF-4. (Ref.: Forrest, MP., et al. (2014). Trends Mol. Med. 20(6); 322-331; Baker, N., et al. (1999). Am. J. Pathol. 154(1); 29-35).
References
Product Information
Format
Purified
Presentation
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Anti-TCF-3/4, clone 6F12, Cat. No. MABE1993, is a mouse monoclonal antibody that detects Transcription factor 3-/4 and is tested for use in Immunocytochemistry, and Immunohistochemistry (Paraffin), and Western Blotting.
Key Applications
Immunocytochemistry
Immunohistochemistry (Paraffin)
Western Blotting
Application Notes
Immunocytochemistry Analysis: A representative lot detected TCF-3/4 in Immunocytochemistry applications (Barker, N., et. al. (1999). Am. J. Pathol. 154(1):29-35).
Immunohistochemistry (Paraffin) Analysis: 1:50 from a representative lot detected TCF-3/4 in human stomach tissue sections.
Immunohistochemistry Applications: A representative lot detected TCF-3/4 in Immunohistochemistry applications (Barker, N., et. al. (1999). Am. J. Pathol. 154(1):29-35).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Biological Information
Immunogen
His-tagged recombinant fragment corresponding to 301 amino acids from the N-terminal half of human TCF-4.
Epitope
N-terminal half
Clone
6F12
Concentration
0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Host
Mouse
Specificity
Clone 6F12 is a mouse monoclonal antibody that detects Transcription factor 4 (TCF4). It targets an epitope within the N-terminal half. Also displays reactivity with TCF3.
~73 kDa observed; 71.31 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance
Evaluated by Western Blotting in Colo-201 cell lysates.
Western Blotting Analysis: A 1:500 dilution of this antibody detected TCF4 in Colo-201 cell lysates.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 1 year at +2°C to +8°C from date of receipt.
Restricted high level expression of Tcf-4 protein in intestinal and mammary gland epithelium N Barker 1 , G Huls, V Korinek, H Clevers Am J Pathol
154(1)
29-35
1998
Tcf-4 is a member of the Tcf/Lef family of transcription factors that interact functionally with beta-catenin to mediate Wnt signaling in vertebrates. We have previously demonstrated that the tumor suppressor function of APC in the small intestine is mediated via regulation of Tcf-4/beta-catenin transcriptional activity. To gain further insight into the role of Tcf-4 in development and carcinogenesis we have generated several mouse monoclonal antibodies, one of which is specific for Tcf-4 and another of which recognizes both Tcf-3 and Tcf-4. Immunohistochemistry performed with the Tcf 4- specific monoclonal antibody revealed high levels of expression in normal intestinal and mammary epithelium and carcinomas derived therefrom. Additional sites of Tcf-3 expression, as revealed by staining with the Tcf-3/-4 antibody, occurred only within the stomach epithelium, hair follicles, and keratinocytes of the skin. A temporal Tcf-4 expression gradient was observed along the crypt-villus axis of human small intestinal epithelium: strong Tcf-4 expression was present within the crypts of early (week 16) human fetal small intestine, with the villi showing barely detectable Tcf-4 protein levels. Tcf-4 expression levels increased dramatically on the villi of more highly developed (week 22) fetal small intestine. We conclude that Tcf-4 exhibits a highly restricted expression pattern related to the developmental stage of the intestinal epithelium. The high levels of Tcf-4 expression in mammary epithelium and mammary carcinomas may also indicate a role in the development of this tissue and breast carcinoma.
