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MAB4301 Anti-Stage-Specific Embryonic Antigen-1 Antibody, clone MC-480

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MAB4301
100 µg  
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      Übersicht

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, RFC, IF, IHC, IPMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMAB4301
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Stage-Specific Embryonic Antigen-1 Antibody, clone MC-480
      Alternate Names
      • SSEA-1
      • CD15
      • Lewis X
      • 3-FAL
      Background InformationSSEA-1 is expressed on the surface of early mouse embryos, murine embryonal carcinoma cells (EC), murine embryonic stem cells (ES) and murine & human germ cells (EG). No immunoreactivity is evident with undifferentiated human EC and ES cells. Expression of SSEA-1 is down regulated following differentiation of murine EC and ES cells. In contrast, the differentiation of human EC and ES cells is characterized by an increase in SSEA-1 expression.
      Product Information
      FormatPurified
      Control
      • P19 mouse embryonic carcinoma cells
      PresentationPurified mouse monoclonal IgM in buffer containing 0.05M Potassium Phosphate, 0.3M NaCl, pH 8.0 with 0.05% Sodium Azide
      Quality LevelMQ100
      Applications
      ApplicationAnti-Stage-Specific Embryonic Antigen-1 Antibody, clone MC-480 detects level of Stage-Specific Embryonic Antigen-1 & has been published & validated for use in FC, IF, IH & IP.
      Key Applications
      • Flow Cytometry
      • Immunofluorescence
      • Immunohistochemistry
      • Immunoprecipitation
      Application NotesImmunohistochemistry:
      A previous lot of this antibody was tested on IH.

      Immunoprecipitation:
      A previous lot of this antibody was tested on IP.

      Immunofluorescence:
      A previous lot of this antibody was tested on IF. 4% PFA fixed, 5-10 minutes room temperature. No detergent is required as epitope is external.

      Flow Cytometry:
      A starting range of 10-20 µg/mL is suggested.

      Positive mouse EC lines include:
      F9, PCC4, ND-1, SCC1, NG2, LT/SV, MH-15, FA-25.
      Lines : PYS-2, OTT6050f, B3T3SV. C57SV, K129SV. KCA, QAIB, BW5147 were negative.

      Optimal working dilutions must be determined by the end user.
      Biological Information
      ImmunogenF9 tetracarcinoma stem cells (X-irradiated).
      CloneMC-480
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityThis antibody reacts with the Stage-specific embryonic antigen-1 (SSEA-1) that is expressed upon the surface of early mouse embryos, murine embryonal carcinoma cells (EC), murine embryonic stem cells (ES) and murine & human germ cells (EG).
      IsotypeIgM
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryFUT4 catalyzes the synthesis of the non-sialylated antigen, Lewis x (CD15). It has been detected in human embryos (5-10 weeks) suggesting a role in development.
      Gene Symbol
      • FUT4
      • ELFT
      • FCT3A
      • Fuc-TIV
      • 1,3fucosyltransferase
      • FUC-TIV
      • FucT-IV
      • CD15
      • Alpha-
      • EC 2.4.1.-
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P22083 # May catalyze alpha-1,3 glycosidic linkages involved in the expression of Lewis X/SSEA-1 and VIM-2 antigens.
      SIZE: 405 amino acids; 45570 Da
      SUBCELLULAR LOCATION: Golgi apparatus, Golgi stack membrane; Single-pass type II membrane protein. Note=Membrane-bound form in trans cisternae of Golgi.
      SIMILARITY: SwissProt: P22083 ## Belongs to the glycosyltransferase 10 family.
      Stem Cell Type
      • Human Embryonic Stem Cells
      • Mouse Embryonic Stem Cells
      • Neural Stem Cells
      • Induced Pluripotent Stem Cells
      Molecular Weight220 kDa
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8ºC from date of receipt.
      Packaging Information
      Material Size100 µg
      Global Trade Item Number
      Bestellnummer GTIN
      MAB4301 04053252396670