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ABS1642 Anti-SPTLC1 Antibody

Anti-SPTLC1 Antibody is an antibody against SPTLC1 for use in Western Blotting.

Anti-SPTLC1 Antibody : SDB (Sicherheitsdatenblätter), Analysenzertifikate und Qualitätszertifikate, Dossiers, Broschüren und andere verfügbare Dokumente.
Research Category: Signaling Research Sub-Category: Lipid Signaling Gene Symbol: SPTLC1, HSAN1, HSAN1A, LCB1 UniProt Number: O15269
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ABS1642
100 µL  
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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWBRbSerumPolyclonal Antibody
      Description
      Catalogue NumberABS1642
      DescriptionAnti-SPTLC1 Antibody
      Alternate Names
      • Serine palmitoyltransferase 1
      • LCB 1
      • Long chain base biosynthesis protein 1
      • Serine-palmitoyl-CoA transferase 1
      • SPT 1
      • SPT1
      Background InformationSerine palmitoyltransferase 1 (EC 2.3.1.50; UniProt O15269; also known as LCB 1, Long chain base biosynthesis protein 1, Serine-palmitoyl-CoA transferase 1, SPT 1, SPT1) is encoded by the SPTLC1 (also known as HSAN1, HSAN1A, LCB1) gene (Gene ID 10558) in human. Serine palmitoyltransferase (SPT) catalyzes pyridoxal 5-phosphate-/PLP-dependent condensation of L-serine with palmitoyl-CoA to generate 3-ketodihydrosphingosine, the rate-limiting step for the de novo synthesis of sphingolipids. SPT belongs to the POAS (PLP-dependent α-oxoamine synthase) family of enzymes, members of this family also include 5-amino-levulinic acid synthase, KBL (2-amino-3 ketobutyrate ligase) and AONS (8-amino-7-oxononanoate synthase). Unlike the other known POAS family members that all exist as soluble homodimers, SPT is bound to the outer membrane of the endoplasmic reticulum (ER) as a multisubunit complex composed of three distinct subunits, SPTLC1, SPTLC2 and SPTLC3. SPTLC1 is a 473-amino acid sigle-transmembrane (a.a. 16-36) protein with a short N-terminal lumenal domain (a.a. 1-15) and a large C-terminal cytoplasmic domain (a.a. 37-473). Alternative splicing generates an isoform with a truncated cytoplasmic domain (D143 switched to E143, a.a. 144-473 truncated).
      References
      Product Information
      FormatSerum
      PresentationRabbit polyclonal antibody serum with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-SPTLC1 Antibody is an antibody against SPTLC1 for use in Western Blotting.
      Key Applications
      • Western Blotting
      Application NotesWestern Blotting Analysis: The purified polyclonal antibody was used to detect the presence of SPTLC1 in SPTLC2 and SPTLC3 immunoprecipiates from human placenta tissue homogenate (Hornemann, T., et al. (2007). Biochem. J. 405(1):157-164).
      Western Blotting Analysis: The purified polyclonal antibody was used to detect SPTLC1 in human cell lysates (HEK293, HepG2, JAR, JEG3) and human placenta tissue homogenate (Hornemann, T., et al. (2006). J. Biol. Chem. 281(49):37275-37281).
      Biological Information
      ImmunogenTwo linear peptides corresponding to sequences from the N- and a C-terminal regions of human SPTLC1 cytoplasmic domain.
      EpitopeCytoplasmic domain.
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityExpected to react with both spliced isoforms of human Serine palmitoyltransferase 1/SPTLC1 reported by UniProt (O15269).
      Species Reactivity
      • Human
      Species Reactivity NoteHuman. Predicted to react with Chimpanzee based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • SPTLC1
      • HSAN1
      • HSAN1A
      • LCB1
      Purification MethodUnpurified
      UniProt Number
      Molecular Weight~51 kDa observed. 52.74 kDa (isoform 1) and 16.07 kDa (isoform 2) calculated.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HepG2 cell lysate.

      Western Blotting Analysis: A 1:5,000 dilution of this antibody detected SPTLC1 in 10 µg of HepG2 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Bestellnummer GTIN
      ABS1642 04055977333428