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487912 Anti-SNAP-25 Rabbit pAb

Anti-SNAP-25 Rabbit pAb: SDB (Sicherheitsdatenblätter), Analysenzertifikate und Qualitätszertifikate, Dossiers, Broschüren und andere verfügbare Dokumente.

Synonyme: Anti-Synaptosomal Associated Protein-25

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      Übersicht

      Replacement Information

      Key Spec Table

      Host
      Rb
      Description
      Overview

      This product has been discontinued.





      Recognizes the ~25 kDa SNAP-25 protein in rat brain membranes.
      Catalogue Number487912
      Brand Family Calbiochem®
      SynonymsAnti-Synaptosomal Associated Protein-25
      References
      ReferencesSollner, T., and Rothman, J.E. 1994. Trends Neurosci. 17, 344
      Oyler, G.A., et al. 1989. J. Cell Biol. 109, 3039.
      Product Information
      FormLyophilized
      Formulation50 µl antibody lyophilized from PBS, pH 7.4, 1% BSA, 5% sucrose and 50 µg lyophilized control protein.
      Positive controlSNAP-25-GST fusion protein or rat brain membranes
      Preservative≤0.1% sodium azide (antibody only)
      Applications
      Application ReferencesFrozen Sections Honer, W.G., et al. 1993. Brain Res. 609 9. Paraffin Sections Honer, W.G., et al. 1994. Acta Neuropathol. 87, 202. Young, C.E., et al. 1998. Cereb. Cortex 8, 261. ELISA and Immunoblotting Young, C.E., et al. 1998. Cereb. Cortex 8, 261.
      Key Applications Immunoblotting (Western Blotting)
      Immunohistochemistry
      Immunoprecipitation
      Application NotesImmunoblotting (1:1000, see application references)
      Frozen Sections (see application references)
      Paraffin Sections (see application references)
      Immunoprecipitation (see comments)
      ELISA (see application references)
      Application CommentsFor a pre-adsorption control use 5 µg positive control protein for each µg of antibody. For immunoblotting use 20 ng positive control protein per lane (minigel). This antibody is also reported to work for immunoprecipitation. Antibody should be titrated for optimal use in individual systems.
      Biological Information
      Immunogena recombinant protein consisting of full-length rat SNAP-25 fused to GST
      ImmunogenRat
      HostRabbit
      IsotypeIgG
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage -20°C
      Do not freeze Ok to freeze
      Special InstructionsReconstitute antibody in 50 µl deionized H₂O to yield a final concentration of 1 mg/ml. Reconstituted antibody may be stored at 4°C for up to 2 weeks. For long-term storage, aliquot and freeze (-20°C). Avoid freeze/thaw cycles of stock solution. Further dilutions should be made with buffer containing 1% BSA. Centrifuge all antibody preparations prior to use. Reconstitute positive control protein with 100 µl PBS. Following reconstitution of control, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Bestellnummer GTIN
      487912 0

      Documentation

      Anti-SNAP-25 Rabbit pAb Analysenzertifikate

      TitelChargennummer
      487912

      Literatur

      Übersicht
      Sollner, T., and Rothman, J.E. 1994. Trends Neurosci. 17, 344
      Oyler, G.A., et al. 1989. J. Cell Biol. 109, 3039.
      Datenblatt

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision21-June-2010 JSW
      SynonymsAnti-Synaptosomal Associated Protein-25
      ApplicationImmunoblotting (1:1000, see application references)
      Frozen Sections (see application references)
      Paraffin Sections (see application references)
      Immunoprecipitation (see comments)
      ELISA (see application references)
      DescriptionRabbit polyclonal antibody pre-adsorbed against immobilized GST, followed by protein A purification. Recognizes the ~25 kDa SNAP-25 protein. Supplied with a positive control consisting of lyophilized SNAP-25-GST fusion protein.
      BackgroundSNAP-25 (synaptosome-associated protein of ~25 kDa) is an integral membrane protein localized to the plasma membrane of pre-synaptic axon terminals. As a component of the receptor for SNAPs (Soluble N-ethylmaleimide-sensitive factor [NSF] Attachment Proteins), SNAP-25 appears to play a critical role in the docking and fusion of pre-synaptic vesicles during exocytosis of neurotransmitters. This anti-SNAP-25 antibody was raised against a highly purified fusion protein of Schistosoma japonicum glutathione-S-transferase (GST) and rat SNAP-25.
      HostRabbit
      Immunogen speciesRat
      Immunogena recombinant protein consisting of full-length rat SNAP-25 fused to GST
      IsotypeIgG
      SpeciesTorpedo sp. (Electric Ray), crayfish, mouse, rat
      Positive controlSNAP-25-GST fusion protein or rat brain membranes
      FormLyophilized
      Formulation50 µl antibody lyophilized from PBS, pH 7.4, 1% BSA, 5% sucrose and 50 µg lyophilized control protein.
      Preservative≤0.1% sodium azide (antibody only)
      CommentsFor a pre-adsorption control use 5 µg positive control protein for each µg of antibody. For immunoblotting use 20 ng positive control protein per lane (minigel). This antibody is also reported to work for immunoprecipitation. Antibody should be titrated for optimal use in individual systems.
      Storage -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsReconstitute antibody in 50 µl deionized H₂O to yield a final concentration of 1 mg/ml. Reconstituted antibody may be stored at 4°C for up to 2 weeks. For long-term storage, aliquot and freeze (-20°C). Avoid freeze/thaw cycles of stock solution. Further dilutions should be made with buffer containing 1% BSA. Centrifuge all antibody preparations prior to use. Reconstitute positive control protein with 100 µl PBS. Following reconstitution of control, aliquot and freeze (-20°C).
      Toxicity Standard Handling
      ReferencesSollner, T., and Rothman, J.E. 1994. Trends Neurosci. 17, 344
      Oyler, G.A., et al. 1989. J. Cell Biol. 109, 3039.
      Application referencesFrozen Sections Honer, W.G., et al. 1993. Brain Res. 609 9. Paraffin Sections Honer, W.G., et al. 1994. Acta Neuropathol. 87, 202. Young, C.E., et al. 1998. Cereb. Cortex 8, 261. ELISA and Immunoblotting Young, C.E., et al. 1998. Cereb. Cortex 8, 261.