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ABF1215 Anti-SLP76 Antibody

This rabbit polyclonal Anti-SLP76, Cat. No. ABF1215 is validated for use in Immunoprecipitation and Western Blotting for the detection of SLP76.

Anti-SLP76 Antibody: SDB (Sicherheitsdatenblätter), Analysenzertifikate und Qualitätszertifikate, Dossiers, Broschüren und andere verfügbare Dokumente.
Research Category: Inflammation & Immunology Gene Symbol: LCP2 UniProt Number: Q13094
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ABF1215
100 µL  
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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, IPRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberABF1215
      Replaces06-548
      DescriptionAnti-SLP76 Antibody
      Alternate Names
      • Lymphocyte cytosolic protein 2
      • SH2 domain-containing leukocyte protein of 76 kDa
      • SLP-76 tyrosine phosphoprotein
      • SLP76
      Background InformationLymphocyte cytosolic protein 2 (UniProt Q13094; also known as SH2 domain-containing leukocyte protein of 76 kDa, SLP-76 tyrosine phosphoprotein, SLP76) is encoded by the LCP2 gene (Gene ID 3937) in human. SLP76 is a signaling adaptor molecule expressed in a variety of hematopoietic cells, including lymphocytes, myeloid cells, and platelets. SLP-76 contains three pYESP motifs (Y113 and Y128) that are phosphorylated by ZAP-70 to produce docking sites for SH2-containing proteins, such as exchange factor Vav1, adaptor Nck, resting lymphocyte kinase Rlk, and inducible tyrosine kinase Itk. In addition, SLP-76 binds to the SH3 domain of phospholipase Cγ1 (PLCγ1) and SLP76-deficient T cells show an impaired PLCγ1 activation and calcium mobilization. SLP-76 is essential for mediating TCR signaling, Lcp2-/- mice exhibit impaired thymic development and their T cells fail to develop beyond stage III at the double-negative stage. SLP76 is also a critical determinant of NK-cell development and the the underlying “missing-self” recognition mechanism that prevents NK cells from destroying cells that express self major histocompatibility complex (MHC) class I molecules. SLP76 contains an N-terminal sterile-α motif or SAM (human a.a. 15-81 and moust a.a. 12-78) and a C-terminal SH2 domain (human/mouse a.a. 422-530).
      References
      Product Information
      FormatAffinity Purified
      PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationThis rabbit polyclonal Anti-SLP76, Cat. No. ABF1215 is validated for use in Immunoprecipitation and Western Blotting for the detection of SLP76.
      Key Applications
      • Western Blotting
      • Immunoprecipitation
      Application NotesImmunoprecipitation Analysis: 10 µL from a representative lot immunoprecipitated SLP76 from 500 µg of Jurkat cell lysate.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to a central region sequence within the N-terminal half of human SLP76.
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityThis rabbit polyclonal antibody targets a central region sequence within the N-terminal half of human SLP76.
      Species Reactivity
      • Human
      Species Reactivity NoteHuman. Predicted to react with Rhesus Macaque based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • LCP2
      Purification MethodAffinity purified.
      UniProt Number
      Molecular Weight~76 kDa observed. 60.19 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in Jurkat cell lysate.

      Western Blotting Analysis: A 1:5,000 dilution of this antibody detected SLP76 in 10 µg of Jurkat cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Bestellnummer GTIN
      ABF1215 04054839083594