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566310 Anti-SIRPα1, C-Terminal (487-503) Rabbit pAb

This Anti-SIRPα1, C-Terminal (487-503) Rabbit pAb is validated for use in Immunoblotting for the detection of SIRPα1, C-Terminal (487-503).

Anti-SIRPα1, C-Terminal (487-503) Rabbit pAb: SDB (Sicherheitsdatenblätter), Analysenzertifikate und Qualitätszertifikate, Dossiers, Broschüren und andere verfügbare Dokumente.

Synonyme: Anti-BIT, Anti-MyD-1, Anti-p84, Anti-SHPS-1

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      Übersicht

      Replacement Information

      Key Spec Table

      Species ReactivityHostAntibody Type
      HRbPolyclonal Antibody
      Description
      Overview

      This product has been discontinued.



      Recognizes the ~75-110 kDa SIRPα and SIRPα1 proteins in THP cells.
      Catalogue Number566310
      Brand Family Calbiochem®
      SynonymsAnti-BIT, Anti-MyD-1, Anti-p84, Anti-SHPS-1
      Application Data
      Detection of human C-terminal SIRPα1 by immunoblotting. Sample: Whole cell lysate from THP-1 cells. Primary antibody: Anti-SIRPα1, C-Terminal (487-503) Rabbit pAb (Cat. No. 566310) (1:2000). Detection: chemiluminescence.
      References
      ReferencesStofega, M.R., et al. 1998. J. Biol. Chem. 273, 7112.
      Kharitonenkov, A., et al. 1997. Nature 386, 181.
      Product Information
      FormLiquid
      FormulationIn PBS.
      Positive controlTHP-1 cells
      Applications
      Key Applications Immunoblotting (Western Blotting)
      Application NotesImmunoblotting (1:1000-1:2000, colorimetric)
      Application CommentsVariables associated with assay conditions will dictate the proper working dilution.
      Biological Information
      Immunogena synthetic peptide [(C)KPEPSFSEYASVQVPRK] corresponding to amino acids 487-503 of human SIRPα1, conjugated to KLH
      ImmunogenHuman
      HostRabbit
      IsotypeIgG
      Species Reactivity
      • Human
      Antibody TypePolyclonal Antibody
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Bestellnummer GTIN
      566310 0

      Documentation

      Anti-SIRPα1, C-Terminal (487-503) Rabbit pAb Analysenzertifikate

      TitelChargennummer
      566310

      Literatur

      Übersicht
      Stofega, M.R., et al. 1998. J. Biol. Chem. 273, 7112.
      Kharitonenkov, A., et al. 1997. Nature 386, 181.
      Datenblatt

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision15-August-2007 RFH
      SynonymsAnti-BIT, Anti-MyD-1, Anti-p84, Anti-SHPS-1
      ApplicationImmunoblotting (1:1000-1:2000, colorimetric)
      Application Data
      Detection of human C-terminal SIRPα1 by immunoblotting. Sample: Whole cell lysate from THP-1 cells. Primary antibody: Anti-SIRPα1, C-Terminal (487-503) Rabbit pAb (Cat. No. 566310) (1:2000). Detection: chemiluminescence.
      DescriptionPurified rabbit polyclonal antibody. Recognizes the ~75-110 kDa SIRPα and SIRPα1 proteins.
      BackgroundSIRPα is a novel SHP-2-associated glycoprotein that is a substrate of many activated tyrosine kinases such as EGFR, insulin receptor, PDGFR, and Src. SIRPα has regulatory effects on cellular responses induced by serum, growth factors, insulin, oncogenes, growth hormones, and cell adhesion and plays a general role in other physiological and pathological processes.
      HostRabbit
      Immunogen speciesHuman
      Immunogena synthetic peptide [(C)KPEPSFSEYASVQVPRK] corresponding to amino acids 487-503 of human SIRPα1, conjugated to KLH
      IsotypeIgG
      Specieshuman
      Positive controlTHP-1 cells
      FormLiquid
      FormulationIn PBS.
      Concentration Label Please refer to vial label for lot-specific concentration
      CommentsVariables associated with assay conditions will dictate the proper working dilution.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Standard Handling
      ReferencesStofega, M.R., et al. 1998. J. Biol. Chem. 273, 7112.
      Kharitonenkov, A., et al. 1997. Nature 386, 181.