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NA19L Anti-Replication Protein A (Ab-3) Mouse mAb (RPA34-20)

SDB (Sicherheitsdatenblätter), Analysenzertifikate und Qualitätszertifikate, Dossiers, Broschüren und andere verfügbare Dokumente.

Synonyme: Anti-RP-A

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      Übersicht

      Replacement Information

      Key Spec Table

      Species ReactivityHostAntibody Type
      H, M, YeastMMonoclonal Antibody
      Description
      Overview

      This product has been discontinued.



      Recognizes the complexed (phosphorylated) and unphosphorylated subunits of replication protein A in HeLa and U293 cells and colon carcinoma tissue.
      Catalogue NumberNA19L
      Brand Family Calbiochem®
      SynonymsAnti-RP-A
      Application Data
      Detection of human replication protein A by immunocytochemical staining. Sample: HeLa cells fixed with methanol. Primary antibody: Anti-Replication Protein A, Human (Mouse) (Cat. No. NA19L) (1.25 μg/ml). Detection: fluorescence.
      References
      ReferencesDin, S., et al. 1990. Genes Dev. 4, 968.
      Brill, S.J. and Stillman, B. 1989. Nature 342, 92.
      Stillman, B., 1989. Annu. Rev. Cell. Biol. 5, 197.
      Tsurimoto, T. and Stillman, B. 1989. EMBO J. 8, 3883.
      Wobbe, C.R., et al. 1987. Proc. Natl. Acad. Sci. 84, 1834.
      Product Information
      FormLyophilized
      FormulationLyophilized from 20 mM ammonium bicarbonate solution, 100 µg BSA.
      Positive controlHeLa or U293 cells or colon carcinoma tissue
      PreservativeNone
      Quality LevelMQ100
      Applications
      Key Applications Immunoblotting (Western Blotting)
      Immunofluorescence
      Immunoprecipitation
      Paraffin Sections
      Application NotesImmunoblotting (5 µg/ml)
      Immunofluorescence (2.5 µg/ml)
      Immunoprecipitation (1 µg/reaction)
      Paraffin Sections (2.5 µg/ml, heat pre-treatment required)
      Application CommentsReplication protein A (Ab-3) will react with both the complexed (phosphorylated) form of the protein as well as the free (unphosphorylated) 34 kDa subunit. Replication protein A can be used as a marker for cell division. Antibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogenreplication protein A purified from U293 cells
      ImmunogenHuman
      Epitopewithin the p34 subunit of replication protein A
      CloneRPA34-20
      HostMouse
      IsotypeIgG2a
      Species Reactivity
      • Human
      • Mouse
      • Yeast
      Antibody TypeMonoclonal Antibody
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Do not freeze Ok to freeze
      Special InstructionsWe recommend resuspending the lyophilized antibody with sterile phosphate buffered saline (PBS), pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4, to yield a final concentration of 100 µg/ml; product will be more stable if 0.1% sodium azide is included (do not add azide if antibody is to be used with viable cells). Lyophilized antibody should be resuspended at 4°C with occasional gentle mixing for at least 2 h.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Bestellnummer GTIN
      NA19L 0

      Documentation

      Anti-Replication Protein A (Ab-3) Mouse mAb (RPA34-20) SDB

      Titel

      Sicherheitsdatenblatt (SDB) 

      Anti-Replication Protein A (Ab-3) Mouse mAb (RPA34-20) Analysenzertifikate

      TitelChargennummer
      NA19L

      Literatur

      Übersicht
      Din, S., et al. 1990. Genes Dev. 4, 968.
      Brill, S.J. and Stillman, B. 1989. Nature 342, 92.
      Stillman, B., 1989. Annu. Rev. Cell. Biol. 5, 197.
      Tsurimoto, T. and Stillman, B. 1989. EMBO J. 8, 3883.
      Wobbe, C.R., et al. 1987. Proc. Natl. Acad. Sci. 84, 1834.
      Datenblatt

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision04-September-2007 RFH
      SynonymsAnti-RP-A
      ApplicationImmunoblotting (5 µg/ml)
      Immunofluorescence (2.5 µg/ml)
      Immunoprecipitation (1 µg/reaction)
      Paraffin Sections (2.5 µg/ml, heat pre-treatment required)
      Application Data
      Detection of human replication protein A by immunocytochemical staining. Sample: HeLa cells fixed with methanol. Primary antibody: Anti-Replication Protein A, Human (Mouse) (Cat. No. NA19L) (1.25 μg/ml). Detection: fluorescence.
      DescriptionPurified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with NS1 mouse myeloma cells. Recognizes the complexed (phosphorylated) and the ~34 kDa unphosphorylated subunit of replication protein A.
      BackgroundThe replication system of a cell contains several essential components among which are replication factor A (RFA, RPA, or HeLa cell SSB), PCNA, and replication factor C (RFC) as well as several other proteins. RPA, a multi-subunit protein containing a 70 kDa, 34 kDa, and 11 kDa protein, may play a critical regulatory role in replication based on its functioning during both the initiation and elongation stages of DNA replication. The protein is highly conserved, having been identified in a range of species from yeast (Saccharomyces cerevisiae) to humans. The primary function of RPA appears to be as an auxiliary protein for both DNA polymerase α and δ. RPA undergoes a cell cycle dependent phosphorylation, which is restricted to the p34 protein. In addition to existing as part of the multi-subunit structure, p34 has also been identified as being present in a free, unphosphorylated form. Recent data shows that RPA is restricted to the nucleus and is overexpressed in transformed cells as well as in several tumor cells.
      HostMouse
      Immunogen speciesHuman
      Immunogenreplication protein A purified from U293 cells
      Epitopewithin the p34 subunit of replication protein A
      CloneRPA34-20
      IsotypeIgG2a
      Specieshuman, mouse, yeast
      Positive controlHeLa or U293 cells or colon carcinoma tissue
      FormLyophilized
      FormulationLyophilized from 20 mM ammonium bicarbonate solution, 100 µg BSA.
      PreservativeNone
      CommentsReplication protein A (Ab-3) will react with both the complexed (phosphorylated) form of the protein as well as the free (unphosphorylated) 34 kDa subunit. Replication protein A can be used as a marker for cell division. Antibody should be titrated for optimal results in individual systems.
      Storage +2°C to +8°C
      Do Not Freeze Ok to freeze
      Special InstructionsWe recommend resuspending the lyophilized antibody with sterile phosphate buffered saline (PBS), pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4, to yield a final concentration of 100 µg/ml; product will be more stable if 0.1% sodium azide is included (do not add azide if antibody is to be used with viable cells). Lyophilized antibody should be resuspended at 4°C with occasional gentle mixing for at least 2 h.
      Toxicity Standard Handling
      ReferencesDin, S., et al. 1990. Genes Dev. 4, 968.
      Brill, S.J. and Stillman, B. 1989. Nature 342, 92.
      Stillman, B., 1989. Annu. Rev. Cell. Biol. 5, 197.
      Tsurimoto, T. and Stillman, B. 1989. EMBO J. 8, 3883.
      Wobbe, C.R., et al. 1987. Proc. Natl. Acad. Sci. 84, 1834.