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MABC1564 Anti-Pin1 Antibody, clone 5B4.1

Anti-Pin1, clone 5B4.1, Cat. No. MABC1564, is a mouse monoclonal antibody that detects Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1 and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.

Anti-Pin1 Antibody, clone 5B4.1: SDB (Sicherheitsdatenblätter), Analysenzertifikate und Qualitätszertifikate, Dossiers, Broschüren und andere verfügbare Dokumente.
Research Category: Apoptosis & Cancer Gene Symbol: PIN1 UniProt Number: Q13526
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MABC1564
100 μg  
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      Übersicht

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, RWB, IH(P)MPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABC1564
      DescriptionAnti-Pin1 Antibody, clone 5B4.1
      Alternate Names
      • Pin1
      • Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1
      • EC: 5.2.1.8
      • Peptidyl-prolyl cis-trans isomerase Pin1
      • PPIase Pin1
      • Rotamase Pin1
      Background InformationPeptidyl-prolyl cis-trans isomerase NIMA-interacting 1 (UniProt: Q13526; also known as EC: 5.2.1.8, Peptidyl-prolyl cis-trans isomerase Pin1, PPIase Pin1, Rotamase Pin1) is encoded by the PIN1 gene (Gene ID: 5300) in human. PPIase binds to and isomerizes specific phosphorylated Ser/Thr-Pro (pSer/Thr-Pro) motifs. Isomerization of phosphorylated Ser or Thr residues may alter protein confirmation and, subsequently, modify activity. By inducing conformational changes in a subset of phosphorylated proteins, it acts as a molecular switch in multiple cellular processes. PPIase is mainly localized in the nucleus, but phosphorylation at Serine 71 by death-associated protein kinase 1 (DAPK1) results in inhibition of its nuclear localization and its catalytic activity. It contains a WW domain (aa 5-39) and a peptidylprolyl isomerase C (PpiC) domain (aa 52-163). WW domain is shown to be essential for the interaction with STIL and KIF20B. PPIase displays a preference for acidic residues located N-terminally to the proline bond to be isomerized. PPIase is reported to regulate mitosis presumably by interacting with NIMA and attenuating its mitosis-promoting activity. It is overexpressed in many human breast cancers, and has been shown to modify numerous proteins including p53, NF-kB, c-Jun, cyclin D1, and beta-catenin.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Pin1, clone 5B4.1, Cat. No. MABC1564, is a mouse monoclonal antibody that detects Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1 and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.
      Key Applications
      • Western Blotting
      • Immunohistochemistry (Paraffin)
      Application NotesImmunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Pin1 in human cerebral cortex tissue sections.
      Biological Information
      ImmunogenGST/His-tagged recombinant fragment corresponding to 92 amino acids from the C-terminal half of human Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1.
      Clone5B4.1
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone 5B4.1 detects Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1 (Pin1) in human and rat cells. It targets an epitope within 92 amino acids from the C-terminal half.
      IsotypeIgG2aκ
      Species Reactivity
      • Human
      • Rat
      Species Reactivity NoteHuman, Rat
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • PIN1
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~18 kDa observed; 18.24 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in PC-12 cell lysate.

      Western Blotting Analysis: 1 µg/mL of this antibody detected Pin1 in 10 µg of PC-12 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Bestellnummer GTIN
      MABC1564 04054839396649