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Wählen Sie konfigurierbare Panels & Premixed-Kits - ODER - Kits für die zelluläre Signaltransduktion & MAPmates™
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Die folgenden MAPmates™ sollten nicht zusammen analysiert werden: -MAPmates™, die einen unterschiedlichen Assaypuffer erfordern. -Phosphospezifische und MAPmate™ Gesamtkombinationen wie Gesamt-GSK3β und Gesamt-GSK3β (Ser 9). -PanTyr und locusspezifische MAPmates™, z.B. Phospho-EGF-Rezeptor und Phospho-STAT1 (Tyr701). -Mehr als 1 Phospho-MAPmate™ für ein einziges Target (Akt, STAT3). -GAPDH und β-Tubulin können nicht mit Kits oder MAPmates™, die panTyr enthalten, analysiert werden.
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48-602MAG
Buffer Detection Kit for Magnetic Beads
1 Kit
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Detect PSD93 using this Anti-PSD93 Antibody, clone N18/30 validated for use in Western Blotting & IHC.
More>>Detect PSD93 using this Anti-PSD93 Antibody, clone N18/30 validated for use in Western Blotting & IHC. Less<<
Anti-PSD93 Antibody, clone N18/30: SDB (Sicherheitsdatenblätter), Analysenzertifikate und Qualitätszertifikate, Dossiers, Broschüren und andere verfügbare Dokumente.
As a member of the membrane-associated guanylate kinase (MAGUK) family, PSD93 (also known as Chapsyn-110) binds directly to the N-methyl-D-aspartate (NMDA) receptor and the Shaker K+ channel subunits. It shares 70-80% homology with PSD-95 (SAP90), and SAP97. Like PSD-95, PSD93 plays a key role in postsynaptic targeting. Aside from binding to glutamate receptors, the MAGUK family of proteins also interacts with cell surface structures, like cell adhesion molecules, ion channels, cytoskeletal elements, and signal transduction molecules. Improper PSD93 signaling has been implicated in certain disease states, such as schizophrenia.
References
Product Information
Format
Purified
Control
Mouse brain tissue lysate
Presentation
Purified mouse monoclonal IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Detect PSD93 using this Anti-PSD93 Antibody, clone N18/30 validated for use in Western Blotting & IHC.
Key Applications
Western Blotting
Immunohistochemistry
Application Notes
Western Blot Analysis: 1 µg/mL from a representative lot detected PSD93 in 10 µg of human brain tissue lysate.
Immunohistochemistry Analysis: A representative lot detected PSD93 in rat brain tissue (Prof. J. Trimmer, University of California, Davis).
Western Blot Analyis: A representative lot detected PSD93 in WT, but not in PSD93 knockout mice.
Western Blot Analysis: A representative lot detected PSD93 in CA1 tissue lysates from C57BL/6 mice (Jeffrey, R. A., et al. (2009). J Neurosci. 29(50):15613-15620.).
Biological Information
Immunogen
Recombinant protein corresponding to rat PSD93.
Clone
N18/30
Concentration
Please refer to the Certificate of Analysis for the lot-specific concentration.
Host
Mouse
Isotype
IgG1κ
Species Reactivity
Mouse
Human
Rat
Species Reactivity Note
Demonstrated to react with Mouse, Human, and Rat. This antibody does not demonstrate cross reactivity with other MAGUK family members, such as PSD-95, SAP97, and SAP102 expressed in transfected cells.
~110 kDa observed. The calculated molecular weight of PSD93 is 95 kDa; however, this protein is highly phosphorylated and is observed at ~110 kDa. Multiple isoforms between 68-98 kDa are known to exist.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance
Evaluated by Western Blot in mouse brain tissue lysate.
Western Blot Analysis: 1 µg/mL of this antibody detected PSD93 in 10 µg of mouse brain tissue lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Activity-dependent anchoring of importin alpha at the synapse involves regulated binding to the cytoplasmic tail of the NR1-1a subunit of the NMDA receptor. Jeffrey, Rachel A, et al. J. Neurosci., 29: 15613-20 (2009)
2009
Synaptic plasticity, the capacity of neurons to change the strength of their connections with experience, provides a mechanism for learning and memory in the brain. Long-term plasticity requires new transcription, indicating that synaptically generated signals must be transported to the nucleus. Previous studies have described a role for importin nuclear transport adaptors in mediating the retrograde transport of signals from synapse to nucleus during plasticity. Here, we investigated the possibility that stimulus-induced translocation of importins from synapse to nucleus involves activity-dependent anchoring of importins at the synapse. We show that importin alpha binds to a nuclear localization signal (NLS) present in the cytoplasmic tail of NR1-1a. This interaction is disrupted by activation of NMDA receptors in cultured neurons and by stimuli that trigger late-phase, but not early-phase, long-term potentiation of CA3-CA1 synapses in acute hippocampal slices. In vitro PKC phosphorylation of GST-NR1-1a abolishes its ability to bind importin alpha in brain lysates, and the interaction of importin alpha and NR1 in neurons is modulated by PKC activity. Together, our results indicate that importin alpha is tethered at the postsynaptic density by binding to the NLS present in NR1-1a. This interaction is activity dependent, with importin alpha being released following NMDA receptor activation and phosphorylation rendering it available to bind soluble cargoes and transport them to the nucleus during transcription-dependent forms of neuronal plasticity.
The nervous system coordinates the voluntary and involuntary actions of the individual and transmits signals between different parts of the body. Weitere Informationen >>
