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07-1221 Anti-PP2A-B’ (B56) Antibody

Detect PP2A-B’ (B56) using this Anti-PP2A-B’ (B56) Antibody validated for use in WB.

Anti-PP2A-B’ (B56) Antibody: SDB (Sicherheitsdatenblätter), Analysenzertifikate und Qualitätszertifikate, Dossiers, Broschüren und andere verfügbare Dokumente.
Research Category: Signaling Research Sub-Category: PI3K, Akt, & mTOR Signaling, Kinases & Phosphatases Gene Symbol: MGC2184 , OTTHUMP00000022333, PP2A , PR53 , PTPA UniProt Number: Q15257
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07-1221
100 µg  
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      Übersicht

      Replacement Information
      Replacement Information07-1221 is a recommended replacement for AB4088

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      B, H, M, RWBRbPurifiedPolyclonal Antibody
      Description
      Catalogue Number07-1221
      ReplacesAB4088
      DescriptionAnti-PP2A-B’ (B56) Antibody
      Alternate Names
      • PP2A phosphatase activator
      • PP2A, subunit B
      • PP2A, subunit B', PR53 isoform
      • Phosphotyrosyl phosphatase activator
      • protein phosphatase 2A activator, regulatory subunit 4
      • protein phosphatase 2A, regulatory subunit B
      • protein phosphatase 2A, regulatory subunit B' (PR 53)
      Background InformationPP2A is a heterotrimeric type 2 Ser/Thr phosphatase, composed of a structural subunit (A), a regulatory subunit (B), and a catalytic subunit (C). The structural A and catalytic C subunits form the constitutive core of the enzyme, which associates with one of a large number of regulatory B subunits. There are multiple isotypes (encoded by distinct genes) of the B subunit, grouped into three families: B/PR55, B'/PR61, and B''/PR72 . The prototypical B (or PR55) family of subunits is encoded by three genes, Balpha, Bbeta and Bgamma . The B' (or B56) family consists of at least seven splice variants derived from five genes. RNA's for the two members of the B'' family, PR72 and PR130, arise by alternative splicing or use of alternate transcription start sites from the same gene. Primary structures as well as functions of B and B' subunits are conserved from yeast to mammals. PTPA is a member of the B' family that is required for activation of PP2A by an undefined mechanism. PP2A is distinguishable from PP2B/Calcineurin and PP2C by its partial activity in the absence of divalent cations, and its high sensitivity to Okadaic Acid. It is also thought to be the principal downmodulator of Ser/Thr kinase cascades.
      References
      Product Information
      FormatPurified
      Control
      • HeLa Cell Lysate
      PresentationPurified rabbit polyclonal IgG in PBS with 0.08% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationDetect PP2A-B’ (B56) using this Anti-PP2A-B’ (B56) Antibody validated for use in WB.
      Key Applications
      • Western Blotting
      Biological Information
      ImmunogenThe immunogen used for the purified peptide conjugated to KLH corresponds to the sequence NH2-Val-Ser-Ser-Pro-His-Phe-Gln-Val-Ala-Glu- Arg-Ala-Leu-Tyr-COOH.
      EpitopeInternal
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityThis antibody is specific to PP2A-B’ subunits.
      IsotypeIgG
      Species Reactivity
      • Bovine
      • Human
      • Mouse
      • Rat
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe product of this gene belongs to the phosphatase 2A regulatory subunit B family. Protein phosphatase 2A is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. The B regulatory subunit might modulate substrate selectivity and catalytic activity. This gene encodes an epsilon isoform of the regulatory subunit B56 subfamily.
      Gene Symbol
      • MGC2184
      • OTTHUMP00000022333
      • PP2A
      • PR53
      • PTPA
      Purification MethodPurified
      UniProt Number
      UniProt SummaryFUNCTION: Reversibly stimulates the variable phosphotyrosyl phosphatase activity of PP2A core heterodimer in presence of ATP and Mg2+ (in vitro).
      SUBUNIT STRUCTURE:Associates with PP2A heterodimeric core enzyme, composed of a 36 kDa catalytic subunit (subunit C) and a 65 kDa constant regulatory subunit (PR65 or subunit A).
      TISUE SPECIFICITY: Widely expressed.
      SEQUENCE SIMILARITY: Belongs to the PTPA-type PPIase family.
      Molecular Weight~56 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by Western Blot on HeLa lysates.

      Western Blot Analysis: 1:500-1:1,500 dilution of this antibody detected PP2A-B' (B56) in 10 μg of multiple cell lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain at -20°C in undiluted aliquots for up to 1 year from date of receipt.
      Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Bestellnummer GTIN
      07-1221 04053252324192

      Documentation

      Anti-PP2A-B’ (B56) Antibody SDB

      Titel

      Sicherheitsdatenblatt (SDB) 

      Anti-PP2A-B’ (B56) Antibody Analysenzertifikate

      TitelChargennummer
      Anti-PP2A-B#8217; (B56) - 2148310 2148310
      Anti-PP2A-B#8217; (B56) - NG1590626 NG1590626
      Anti-PP2A-B#8217; (B56) - NG1653545 NG1653545
      Anti-PP2A-B#8217; (B56) - NG1698984 NG1698984
      Anti-PP2A-B#8217; (B56) - NG1752512 NG1752512
      Anti-PP2A-B#8217; (B56) - NG1882237 NG1882237
      Anti-PP2A-B’ (B56) 3077398
      Anti-PP2A-B’ (B56) - 3170226 3170226
      Anti-PP2A-B’ (B56) - 3615440 3615440
      Anti-PP2A-B’ (B56) -2608440 2608440
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