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DR1108 Anti-POLI Mouse mAb (8G9)

Anti-POLI Mouse mAb (8G9): SDB (Sicherheitsdatenblätter), Analysenzertifikate und Qualitätszertifikate, Dossiers, Broschüren und andere verfügbare Dokumente.

Synonyme: Anti-Polymerase DNA-Directed ι

DR1108
  
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      Übersicht

      Replacement Information

      Key Spec Table

      Host
      M
      Description
      Overview

      This product has been discontinued.





      Recognizes POLI protein in cell lysates.
      Catalogue NumberDR1108
      Brand Family Calbiochem®
      SynonymsAnti-Polymerase DNA-Directed ι
      Application Data
      Detection of human POLI by immunoblotting. Sample: Whole cell lysate from HeLa NE cells. Primary antibody: Anti-POLI Mouse mAb (8G9) (Cat. No. DR1108) (1-5 µg/ml). Detection: chemilimunescence.
      References
      ReferencesWang, Y., et al. 2007. Cancer Res. 67 3018.
      Product Information
      FormLiquid
      FormulationIn PBS, pH 7.2.
      Negative control293T cells
      Positive controlHeLa NE cells
      PreservativeNone
      Applications
      Key Applications Enzyme-Linked Immunosorbent Assay
      Immunoblotting (Western Blotting)
      Application NotesELISA (see coments) Immunoblotting (1-5 µg/ml)
      Application CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
      Biological Information
      ImmunogenA recombinant polypeptide corresponding to amino acids of 616-716 human POLI, expressed as a GST fusion protein
      Clone8G9
      HostMouse
      IsotypeIgG₃
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Regulatory Review
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Bestellnummer GTIN
      DR1108 0

      Documentation

      Literatur

      Übersicht
      Wang, Y., et al. 2007. Cancer Res. 67 3018.
      Datenblatt

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision05-November-2010 RFH
      SynonymsAnti-Polymerase DNA-Directed ι
      ApplicationELISA (see coments) Immunoblotting (1-5 µg/ml)
      Application Data
      Detection of human POLI by immunoblotting. Sample: Whole cell lysate from HeLa NE cells. Primary antibody: Anti-POLI Mouse mAb (8G9) (Cat. No. DR1108) (1-5 µg/ml). Detection: chemilimunescence.
      DescriptionPurified mouse monoclonal antibody. Recognizes the ~75 kDa POLI protein.
      BackgroundError-prone, DNA polymerase specifically involved in DNA repair. Plays an important role in translesion synthesis, where the normal high-fidelity DNA polymerases cannot proceed and DNA synthesis stalls. Favors Hoogsteen base-pairing in the active site. Inserts the correct base with high-fidelity opposite an adenosine template. Exhibits low fidelity and efficiency opposite a thymidine template, where it will preferentially insert guanosine. May play a role in hypermutation of immunogobulin genes. Forms a Schiff base with 5'-deoxyribose phosphate at abasic sites, but may not have lyase activity.
      HostMouse
      ImmunogenA recombinant polypeptide corresponding to amino acids of 616-716 human POLI, expressed as a GST fusion protein
      Clone8G9
      IsotypeIgG₃
      Specieshuman
      Positive controlHeLa NE cells
      Negative control293T cells
      FormLiquid
      FormulationIn PBS, pH 7.2.
      PreservativeNone
      CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Regulatory Review
      ReferencesWang, Y., et al. 2007. Cancer Res. 67 3018.