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05-163 Anti-PLCγ-1 Antibody

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05-163
50 µg  
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      Übersicht

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, R, Rb, BICC, IP, WBMPurifiedMonoclonal Antibody
      Description
      Catalogue Number05-163
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-PLCγ-1 Antibody
      OverviewSeems to be redundant as it holds the same info as the Presentation.
      Alternate Names
      • 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase gamma 1
      • Phosphoinositide phospholipase C
      • Phospholipase C-gamma-1
      • monophosphatidylinositol phosphodiesterase
      • phosphatidylinositol phospholipase C
      • phosphoinositidase C
      • phospholipase C gamma 1
      • phospholipase C, gamma 1
      • phospholipase C, gamma 1 (formerly subtype 148)
      • phospholipase C-148
      • triphosphoinositide phosphodiesterase
      Background InformationPhospholipase C gamma 1 is a tyrosine kinase substrate for many receptor and nonreceptor tyrosine kinases. Activation of the enzyme produces two second messenger molecules, inositol 1,4,5-trisphosphate and diacylglycerol, which provoke the mobilization of intracellular Ca2+ and activation of protein kinase C. Although the mammalian genome encodes 10 known phosphoinositide specific PLCs, only the gamma 1 and gamma 2 isoforms are regulated by tyrosine kinase activity. PLC beta isoform activity is controlled by heterotrimeric G protein coupled receptors, while the mechanism of regulation of PLCgamma activity is unknown.
      Product Information
      FormatPurified
      Control
      • Positive Antigen Control: Catalog #12-301, non-stimulated A431 cell lysate. Add 2.5µL of 2-mercaptoethanol/100µL of lysate and boil for 5 minutes to reduce the preparation. Load 20µg of reduced lysate per lane for mingels.
      PresentationLiquid in 0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl and 0.05% sodium azide.
      An equal mix of IgG antibodies produced by SP2/0-Ag14 x Balb/c spleen cells (Suh, P.G., 1988) and propagated as purified immunoglobulin. Clones B-2-5, B-6-4, B-20-3, D-7-3, E-9-4.
      Quality LevelMQ100
      Applications
      ApplicationAnti-PLCγ-1 Antibody detects level of PLCγ-1 & has been published & validated for use in IC, IP & WB.
      Key Applications
      • Immunocytochemistry
      • Immunoprecipitation
      • Western Blotting
      Application NotesImmunoprecipitation:
      4 µg of a previous lot of antibody immunoprecipitated PLCγ-1 from non-stimulated A431 cell lysate, previous lots immunoprecipitated PLCγ-1 from rabbit brain cytosol preparation.

      Immunocytochemistry:
      5-10 µg/mL of previous lots of this antibody detected PLCγ-1 on ethanol-acetic acid [95:5] fixed A431 cells.
      Biological Information
      ImmunogenFive individual clones raised against purified soluble phospholipase C (PLC)γ-1 from bovine brain.
      CloneClonal blend B-2-5, B-6-4, B-20-3, D-7-3, E-9-4.
      HostMouse
      SpecificityThis antibody recognizes PLCγ-1, Mr 135 kDa. Reacts with some non-PLCγ-1 SH-3 containing proteins, including nck, Mr 47kDa (Park, D., 1992).
      Isotypemixed IgGs
      Species Reactivity
      • Human
      • Mouse
      • Rat
      • Rabbit
      • Bovine
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene catalyzes the formation of inositol 1,4,5-trisphosphate and diacylglycerol from phosphatidylinositol 4,5-bisphosphate. This reaction uses calcium as a cofactor and plays an important role in the intracellular transduction of receptor-mediated tyrosine kinase activators. For example, when activated by SRC, the encoded protein causes the Ras guanine nucleotide exchange factor RasGRP1 to translocate to the Golgi, where it activates Ras. Also, this protein has been shown to be a major substrate for heparin-binding growth factor 1 (acidic fibroblast growth factor)-activated tyrosine kinase. Two transcript variants encoding different isoforms have been found for this gene.
      Gene Symbol
      • PLCG1
      • PLC1
      • PLCgamma1
      • PLC148
      • PLC-II
      • PLC-148
      • 1-phosphatidyl-D-myo-inositol-4,5-bisphosphate
      • PLC-gamma-1
      • inositoltrisphosphohydrolase
      Purification MethodProtein G Chromatography
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P19174 # PLC-gamma is a major substrate for heparin-binding growth factor 1 (acidic fibroblast growth factor)-activated tyrosine kinase.
      COFACTOR: Calcium.
      SIZE: 1290 amino acids; 148532 Da
      SUBUNIT: Interacts with CENTG1 via its SH3 domain (By similarity). Interacts with phosphorylated LAT upon TCR activation. Interacts with the Pro-rich domain of TNK1 via its SH3 domain. Associates with BLNK, VAV1, GRB2 and NCK1 in a B-cell antigen receptor- dependent fashion. Interacts with CBLB in activated T-cells; which inhibits phosphorylation. Interacts with SHB. Interacts via its SH3 domain with the Arg/Gly-rich-flanked Pro-rich domains of KHDRBS1/SAM68. This interaction is selectively regulated by arginine methylation of KHDRBS1/SAM68. Interacts with INPP5D/SHIP1 (By similarity).
      PTM: The receptor-mediated activation of PLC-gamma-1 and PLC- gamma-2 involves their phosphorylation by tyrosine kinases in response to ligation of a variety of growth factor receptors and immune system receptors. & Ubiquitinated by CBLB in activated T-cells (By similarity).
      SIMILARITY: SwissProt: P19174 ## Contains 1 C2 domain. & Contains 1 EF-hand domain. & Contains 2 PH domains. & Contains 1 PI-PLC X-box domain. & Contains 1 PI-PLC Y-box domain. & Contains 2 SH2 domains. & Contains 1 SH3 domain. ...
      Molecular Weight135 kDa
      Product Usage Statements
      Quality AssuranceRoutinely evaluated in human A431 carcinoma cells, rabbit brain cytosol, and bovine brain cytosol preparations.

      Western Blot Analysis:
      0.5-2 µg/mL of this lot detected PLCγ-1 in human A431 carcinoma cells; previous lots detected PKCγ-1 in rabbit brain cytosol and bovine brain cytosol preparations.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20ºC from date of shipment.
      Packaging Information
      Material Size50 µg
      Global Trade Item Number
      Bestellnummer GTIN
      05-163 04053252580987