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MABS522 Anti-PIKFYVE Antibody, clone R159.4.3C9

Anti-PIKFYVE Antibody, clone R159.4.3C9 is an antibody against PIKFYVE for use in Western Blotting.

Anti-PIKFYVE Antibody, clone R159.4.3C9 : SDB (Sicherheitsdatenblätter), Analysenzertifikate und Qualitätszertifikate, Dossiers, Broschüren und andere verfügbare Dokumente.
Research Category: Signaling Research Sub-Category: General Post-translation Modification Gene Symbol: PIKFYVE, CFD, FAB1, HEL37, KIAA0981, PIP5K, PIP5K3, ZFYVE29 UniProt Number: Q9Y2I7
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MABS522
100 μg  
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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWBMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABS522
      DescriptionAnti-PIKFYVE Antibody, clone R159.4.3C9
      Alternate Names
      • 1-phosphatidylinositol 3-phosphate 5-kinase
      • Epididymis luminal protein 37
      • FYVE finger-containing phosphoinositide kinase
      • PIKfyve
      • Phosphatidylinositol 3-phosphate 5-kinase type III
      • PIPkin-III
      • Type III PIP kinase
      • Zinc finger FYVE domain containing 29
      Background Information1-phosphatidylinositol 3-phosphate 5-kinase (EC 2.7.1.150; UniProt Q9Y2I7; also known as Epididymis luminal protein 37, FYVE finger-containing phosphoinositide kinase, PIKfyve, Phosphatidylinositol 3-phosphate 5-kinase type III, PIPkin-III, Type III PIP kinase, Zinc finger FYVE domain containing 29) is encoded by the PIKFYVE (also known as CFD, FAB1, HEL37, KIAA0981, PIP5K, PIP5K3, ZFYVE29) gene (Gene ID 200576) in human. PIKfyve is a class III lipid kinase that is responsible for phosphorylating phosphatidylinositol (PtdIns) and phosphatidylinositol 3-phosphate (PtdIns3P) of their inositol ring at the D-5 position to form PtdIns3P and PtdIns(3,5)P2 (phosphatidylinositol 3,5-bisphosphate), respectively. PIKfyve forms a stable ternary heterooligomeric complex with its regulator ArPIKfyve (encoded by the VAC14 gene) and the Sac1 domain-containing PtdIns(3,5)P2 5-phosphatase Sac3 (encoded by the FIG4 gene), and both ArPIKfyve and Sac3 are required for maximal PIKfyve activity. PIKfyve is also known to interact with the Rab9 effector RABEPK and the kinesin adaptor JLP (encoded by SPAG9). By regulating endosomal trafficking, PIKfyve plays an important role in multiple biological processes, such as GLUT4 translocation, retroviral budding, autophagy, and neurodegeneration. Human PIKfyve is initially produced as a 2098-amino acid protein whose N-terminal initiator methionine is removed posttranslationally. PIKFYVE gene mutation is linked to corneal fleck dystrophy (CFD) characterized by white flecks scattered in all levels of the stroma.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal IgG2aκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-PIKFYVE Antibody, clone R159.4.3C9 is an antibody against PIKFYVE for use in Western Blotting.
      Key Applications
      • Western Blotting
      Application NotesWestern Blotting Analysis: 1.0 µg/mL from a representative lot detected PIKFYVE in 10 µg of HepG2 cell lysate.
      Biological Information
      ImmunogenAviTag-6xHis-tagged recombinant protein corresponding an internal fragment of human PIKFYVE.
      EpitopeInternal (N-terminal half).
      CloneR159.4.3C9
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityExpected to react with all four spliced isoforms of human PIKfyve reported by UniProt (Q9Y2I7-1 through Q9Y2I7-4).
      IsotypeIgG2aκ
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • PIKFYVE
      • CFD
      • FAB1
      • HEL37
      • KIAA0981
      • PIP5K
      • PIP5K3
      • ZFYVE29
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight~240/50 kDa observed. 237,1 kDa (isoform 1), 50.21 kDa (isoform 2), 51.48 kDa (isoform 3), 61.60 kDa (isoform 4) calculated.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HL-60 cell lysate.

      Western Blotting Analysis: 2.0 µg/mL of this antibody detected PIKFYVE in 20 µg of HL-60 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Bestellnummer GTIN
      MABS522 04055977312478