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ST1698 Anti-PDLIM7 Mouse mAb (2D6)

Anti-PDLIM7 Mouse mAb (2D6): SDB (Sicherheitsdatenblätter), Analysenzertifikate und Qualitätszertifikate, Dossiers, Broschüren und andere verfügbare Dokumente.

Synonyme: Anti-PD2 and LIM Domain7

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ST1698
  
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      Übersicht

      Replacement Information

      Key Spec Table

      Species ReactivityHostAntibody Type
      HMMonoclonal Antibody
      Description
      Overview

      This product has been discontinued.



      Recognizes the ~45-50 kDa PDLIM7 protein in HeLa cells.
      Catalogue NumberST1698
      Brand Family Calbiochem®
      SynonymsAnti-PD2 and LIM Domain7
      Application Data
      Detection of human PDLIM7 by immunoblotting. Sample: Whole cell lysate from HeLa NE cells. Primary antibody: Anti-PDLIM7 Mouse mAb (2D6) (Cat. No. ST1698) (1-5 µg/ml). Detection: chemiluminescence.

      Detection of human PDLIM7 by immunocytochemistry. Sample: HeLa cells fixed in paraformaldehyde. Primary antibody: Anti-PDLIM7 Mouse mAb (2D6) (Cat. No. ST1698) (10 µg/ml). Detection: fluorescence.
      References
      ReferencesHerrick, S., et al. 2009. Mol. Cell Neurosci. 43, 188.
      Product Information
      Formliquid
      FormulationIn PBS, pH 7.2.
      Negative control293T cells
      Positive controlHeLa cells
      PreservativeNone
      Quality LevelMQ100
      Applications
      Key Applications Enzyme-Linked Immunosorbent Assay
      Immunoblotting (Western Blotting)
      Immunocytochemistry
      Application NotesELISA (see coments)
      Immunoblotting (1-5 µg/ml)
      Immunocytochemistry (10 µg/ml)
      Application CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
      Biological Information
      ImmunogenFull-length, recombinant, human PDLIM7 (aa 1-458), expressed as a GST fusion protein
      Clone2D6
      HostMouse
      IsotypeIgG₁
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Regulatory Review
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Bestellnummer GTIN
      ST1698 0

      Documentation

      Anti-PDLIM7 Mouse mAb (2D6) SDB

      Titel

      Sicherheitsdatenblatt (SDB) 

      Literatur

      Übersicht
      Herrick, S., et al. 2009. Mol. Cell Neurosci. 43, 188.
      Datenblatt

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision10-November-2010 RFH
      SynonymsAnti-PD2 and LIM Domain7
      ApplicationELISA (see coments)
      Immunoblotting (1-5 µg/ml)
      Immunocytochemistry (10 µg/ml)
      Application Data
      Detection of human PDLIM7 by immunoblotting. Sample: Whole cell lysate from HeLa NE cells. Primary antibody: Anti-PDLIM7 Mouse mAb (2D6) (Cat. No. ST1698) (1-5 µg/ml). Detection: chemiluminescence.

      Detection of human PDLIM7 by immunocytochemistry. Sample: HeLa cells fixed in paraformaldehyde. Primary antibody: Anti-PDLIM7 Mouse mAb (2D6) (Cat. No. ST1698) (10 µg/ml). Detection: fluorescence.
      DescriptionPurified mouse monoclonal antibody. Recognizes the ~45-50 kDa PDLIM7 protein.
      BackgroundThe protein encoded by this gene is representative of a family of proteins composed of conserved PDZ and LIM domains. LIM domains are proposed to function in protein-protein recognition in a variety of contexts including gene transcription and development and in cytoskeletal interaction. The LIM domains of this protein bind to protein kinases, whereas the PDZ domain binds to actin filaments. The gene product is involved in the assembly of an actin filament-associated complex essential for transmission of ret/ptc2 mitogenic signaling. The biological function is likely to be that of an adapter, with the PDZ domain localizing the LIM-binding proteins to actin filaments of both skeletal muscle and nonmuscle tissues. Alternative splicing of this gene results in multiple transcript variants.
      HostMouse
      ImmunogenFull-length, recombinant, human PDLIM7 (aa 1-458), expressed as a GST fusion protein
      Clone2D6
      IsotypeIgG₁
      Specieshuman
      Positive controlHeLa cells
      Negative control293T cells
      Formliquid
      FormulationIn PBS, pH 7.2.
      PreservativeNone
      CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Regulatory Review
      ReferencesHerrick, S., et al. 2009. Mol. Cell Neurosci. 43, 188.