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Wählen Sie konfigurierbare Panels & Premixed-Kits - ODER - Kits für die zelluläre Signaltransduktion & MAPmates™
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Die folgenden MAPmates™ sollten nicht zusammen analysiert werden: -MAPmates™, die einen unterschiedlichen Assaypuffer erfordern. -Phosphospezifische und MAPmate™ Gesamtkombinationen wie Gesamt-GSK3β und Gesamt-GSK3β (Ser 9). -PanTyr und locusspezifische MAPmates™, z.B. Phospho-EGF-Rezeptor und Phospho-STAT1 (Tyr701). -Mehr als 1 Phospho-MAPmate™ für ein einziges Target (Akt, STAT3). -GAPDH und β-Tubulin können nicht mit Kits oder MAPmates™, die panTyr enthalten, analysiert werden.
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96-Well Plate
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48-602MAG
Buffer Detection Kit for Magnetic Beads
1 Kit
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ABN1675
Sigma-AldrichAnti-Nurr-1
Anti-Nurr-1, Cat. No. ABN1675, is a highly specific rabbit polyclonal antibody that targets Nuclear receptor subfamily 4 group A member 2 and has been tested in ELISA, Immunohistochemistry (Paraffin), and Western Blotting.
More>>Anti-Nurr-1, Cat. No. ABN1675, is a highly specific rabbit polyclonal antibody that targets Nuclear receptor subfamily 4 group A member 2 and has been tested in ELISA, Immunohistochemistry (Paraffin), and Western Blotting. Less<<
Anti-Nurr-1: SDB (Sicherheitsdatenblätter), Analysenzertifikate und Qualitätszertifikate, Dossiers, Broschüren und andere verfügbare Dokumente.
Nuclear receptor subfamily 4 group A member 2 (UniProt: P43354; also known as Immediate-early response protein NOT, Orphan nuclear receptor NURR1, Transcriptionally-inducible nuclear receptor) and is encoded by the NR4A2 (also known as NOT, NURR1, TINUR) gene (Gene ID: 4929) in human. Nurr-1 belongs to the nuclear hormone receptor family that acts as a transcriptional regulator and is considered to be important for the differentiation and maintenance of meso-diencephalic dopaminergic (mdDA) neurons during development. It is also crucial for expression of a set of genes such as SLC6A3, SLC18A2, TH and DRD2 which are essential for development of mdDA neurons. It is expressed in a number of T cell, B cell and fibroblast cell lines, but its expression is higher in brain tissue. Two isoforms of Nurr-1 have been reported that are produced by alternative splicing. The ligand-binding domain (LBD) of Nurr-1 contains no cavity as a result of the tight packing of side chains from several bulky hydrophobic residues in the region normally occupied by ligands. It also lacks a classical binding site for co-activators. However, it has hydrophobic region on the surface of LBD, which is critical for its activity. (Ref.: Volakakis N et al (2006). J. Mol. Endocrinol 37(2); 317-326; Wang G et al. (2003). Nature 423(6939):555-560).
References
Product Information
Format
Unpurified
Presentation
Rabbit polyclonal antiserum in PBS with 0.05% sodium azide.
Anti-Nurr-1, Cat. No. ABN1675, is a highly specific rabbit polyclonal antibody that targets Nuclear receptor subfamily 4 group A member 2 and has been tested in ELISA, Immunohistochemistry (Paraffin), and Western Blotting.
Key Applications
ELISA
Immunohistochemistry (Paraffin)
Western Blotting
Application Notes
Immunohistochemistry (Paraffin) Analysis: A 1:50 dilution from a representative lot detected Nurr-1 in mouse hippocampus and human cerebellum tissue sections.
ELISA Analysis: A representative lot detected recombinant Nurr-1 LBDs (Courtesy of Dr. Pierre Leblanc at McLean Hospital, Belmont, MA).
Immunohistochemistry Analysis: A representative lot detected Nurr-1 in Midbrain dopamine neurons (Leblanc, P., et. al. (2015). J Vis Exp. (102):e52963).
Western Blotting Analysis: A 1:2,000 dilution from a representative lot detected Nurr-1 in HEK293T and CHO cells overexpressed with Myc-Tagged mouse full length Nurr1/Nor1/Nurr77 (Courtesy of Dr. Pierre Leblanc at McLean Hospital, Belmont, MA).
Immunohistochemistry Analysis: A 1:250-1:2,500 dilution from a representative lot detected Nurr-1 in mouse brain tissue sections.(Courtesy of Dr. Pierre Leblanc at McLean Hospital, Belmont, MA).
ELISA Analysis: A representative lot detected Nurr-1 ligand binding domain (Leblanc, P., et. al. (2015). J Vis Exp. (102):e52963).
Western Blotting Analysis: A representative lot detected Nurr-1 in CHO cells transfected with full-length myc-tagged Nurr-1 (Leblanc, P., et. al. (2015). J Vis Exp. (102):e52963).
Biological Information
Immunogen
His-tagged recombinant fragment corresponding to 271 amino acids from the C-terminal half of human Nurr-1 and includes the ligand binding domain.
Epitope
domain of:
Concentration
Please refer to lot specific datasheet.
Host
Rabbit
Specificity
This rabbit polyclonal antibody detects Nurr-1 in human and murine tissues. It targets an epitope within the C-terminal half.
Isotype
IgG
Species Reactivity
Human
Mouse
Species Reactivity Note
Human, Mouse. Predicted to react with Rat based on 100% sequence homology.
~70 kDa observed; 66.59 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance
Evaluated by Immunohistochemistry in mouse cerebral cortex tissue sections.
Immunohistochemistry (Paraffin) Analysis: A 1:50 dilution of this antibody detected Nurr-1 in mouse cerebral cortex tissue sections.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
