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ST1119 Anti-NOD2/CARD15 Rabbit pAb

Anti-NOD2/CARD15 Rabbit pAb: SDB (Sicherheitsdatenblätter), Analysenzertifikate und Qualitätszertifikate, Dossiers, Broschüren und andere verfügbare Dokumente.

Synonyme: Anti-BLAU, Anti-CARD15, Anti-ACUG

ST1119
  
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      Übersicht

      Replacement Information

      Key Spec Table

      Host
      Rb
      Description
      Overview

      This product has been discontinued.





      Recognizes the ~150 kDa NOD2 protein in Jurkat cells.
      Catalogue NumberST1119
      Brand Family Calbiochem®
      SynonymsAnti-BLAU, Anti-CARD15, Anti-ACUG
      Application Data
      Lane 1: Detection of human NOD2 by immunoblotting. Sample: Whole cell lysate (20 µg) from Jurkat cells. Primary antibody: Anti-NOD2 Rabbit pAb (Cat. No. ST1119) (2 µg/ml). Detection: chemiluminescence. Lane 2. Detection of human NOD2 by immunoprecipitation followed by immunoblotting. Sample: Whole cell lysate (500 µg) from Jurkat cells (lane 2) and negative control sample (lane 3). Antibody for immunoprecipitation: Anti-NOD2 Rabbit pAb (Cat. No. ST1119) (5 µg). Immunoblotting conditions: same as lane 1.
      References
      ReferencesFritz, J.H., et al. 2006. Nat. Immunol. 7, 1250.
      Radford-Smith, G. and Pandeya, N. 2006. World J. Gastroenterol. 12, 7097.
      Product Information
      FormLiquid
      FormulationIn Tris-citrate/phosphate buffer, pH 7.0-8.0.
      Preservative≤0.1% sodium azide
      Applications
      Key Applications Immunoblotting (Western Blotting)
      Immunoprecipitation
      Application NotesImmunoblotting (2 µg/ml)
      Immunoprecipitation (5 µg/500 µg total protein)
      Application CommentsAntibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogena synthetic peptide corresponding to amino acids from human NOD2
      ImmunogenHuman
      HostRabbit
      IsotypeIgG
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Bestellnummer GTIN
      ST1119 0

      Documentation

      Literatur

      Übersicht
      Fritz, J.H., et al. 2006. Nat. Immunol. 7, 1250.
      Radford-Smith, G. and Pandeya, N. 2006. World J. Gastroenterol. 12, 7097.
      Datenblatt

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision25-October-2007 RFH
      SynonymsAnti-BLAU, Anti-CARD15, Anti-ACUG
      ApplicationImmunoblotting (2 µg/ml)
      Immunoprecipitation (5 µg/500 µg total protein)
      Application Data
      Lane 1: Detection of human NOD2 by immunoblotting. Sample: Whole cell lysate (20 µg) from Jurkat cells. Primary antibody: Anti-NOD2 Rabbit pAb (Cat. No. ST1119) (2 µg/ml). Detection: chemiluminescence. Lane 2. Detection of human NOD2 by immunoprecipitation followed by immunoblotting. Sample: Whole cell lysate (500 µg) from Jurkat cells (lane 2) and negative control sample (lane 3). Antibody for immunoprecipitation: Anti-NOD2 Rabbit pAb (Cat. No. ST1119) (5 µg). Immunoblotting conditions: same as lane 1.
      DescriptionImmunoaffinity purified rabbit polyclonal antibody. Recognizes the ~150 kDa NOD2/CARD15 protein.
      BackgroundNod2 (nucleotide-binding oligomerization domain containing 2; accession no. NP 071445) is a 150 kDa protein that is a member of the Nod-like receptor (NLR) family. It is strongly associated with host responses to intracellular invasion by bacteria and inflammatory responses. Mutations in Nod2 have been associated with the inflammatory disorders, Crohn's disease and Blau syndrome. Nod2 contains two caspase recruitment (CARD) domains at the N-terminus, a NACHT domain in the central region, and six leucine-rich repeats (LRRs) at the C-terminus. Bacterial pathogens are recognized by the host cells extracellularly through Toll-like receptors or intracellularly by a family of cytosolic Nod-like receptors (NLRs). NLRs recognize the microbial molecules that are introduced by the pathogens and/or endogenous factors released from damaged tissue. Nod2 detects intracellular presence of muramyl dipeptide, the largest molecular motif common to Gram-negative and Gram-positive bacteria. After detection of the bacterial product, Nod2 forms a complex with receptor-interacting protein 2 (RIP2) through a CARD-CARD interaction. This complex recruits NEMO (IKK-γ) and mediates the ubiquitin-proteasome-dependent degradation of IκBα, which results in the activation and nuclear traslocation of NF-κB and the upregulation of inflammatory mediators.
      HostRabbit
      Immunogen speciesHuman
      Immunogena synthetic peptide corresponding to amino acids from human NOD2
      IsotypeIgG
      Specieshuman
      FormLiquid
      FormulationIn Tris-citrate/phosphate buffer, pH 7.0-8.0.
      Concentration Label Please refer to vial label for lot-specific concentration
      Preservative≤0.1% sodium azide
      CommentsAntibody should be titrated for optimal results in individual systems.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Standard Handling
      ReferencesFritz, J.H., et al. 2006. Nat. Immunol. 7, 1250.
      Radford-Smith, G. and Pandeya, N. 2006. World J. Gastroenterol. 12, 7097.