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06-1029
Sigma-AldrichAnti-NET55 Antibody
Use Anti-NET55 Antibody (Rabbit Polyclonal Antibody) validated in WB, IHC to detect NET55 also known as anterior pharynx defective 1 homolog B (C. elegans) or gamma-secretase subunit APH-1B.
More>>Use Anti-NET55 Antibody (Rabbit Polyclonal Antibody) validated in WB, IHC to detect NET55 also known as anterior pharynx defective 1 homolog B (C. elegans) or gamma-secretase subunit APH-1B. Less<<
Anti-NET55 Antibody: SDB (Sicherheitsdatenblätter), Analysenzertifikate und Qualitätszertifikate, Dossiers, Broschüren und andere verfügbare Dokumente.
anterior pharynx defective 1 homolog B (C. elegans)
presenilin stabilization factor-like
aph-1beta
gamma-secretase subunit APH-1B
anterior pharynx defective 1B-like
Presenilin-stabilization factor-like
Aph-1beta
Background Information
NET55 is more commonly known as gamma-secretase subunit APH-1 beta (APH-1b). APH-1b is a multipass membrane protein that is related with nicastrin and the heterodimers of the presenilin fragments in human cell lines and rat brain. APH-1b is a part of the gamma-secretase enzyme compound which is responsible for neurodevelopmental signaling pathways.
References
Product Information
Format
Affinity Purified
Control
Rat liver tissue lysate
Presentation
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Applications
Application
Use Anti-NET55 Antibody (Rabbit Polyclonal Antibody) validated in WB, IHC to detect NET55 also known as anterior pharynx defective 1 homolog B (C. elegans) or gamma-secretase subunit APH-1B.
Key Applications
Western Blotting
Immunohistochemistry
Application Notes
Immunohistochemistry Analysis: 1:500 dilution from a representative lot detected NET55 in mouse brain tissue.
Biological Information
Immunogen
KLH-conjugated linear peptide corresponding to human NET55.
Concentration
Please refer to the Certificate of Analysis for the lot-specific concentration.
APH1 is a multipass transmembrane protein that interacts with presenilin (see PSEN1; MIM 104311) and nicastrin (APH2; MIM 605254) as a functional component of the gamma-secretase complex. The gamma-secretase complex is required for the intramembrane proteolysis of a number of membrane proteins, including the amyloid-beta precursor protein (APP; MIM 104760) and Notch (MIM 190198).[supplied by OMIM].
FUNCTION: Probable subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral proteins such as Notch receptors and APP (beta-amyloid precursor protein). It probably represents a stabilizing cofactor for the presenilin homodimer that promotes the formation of a stable complex. Probably present in a minority of gamma-secretase complexes compared to APH1A.
SUBUNIT STRUCTURE: Probable component of the gamma-secretase complex, a complex composed of a presenilin homodimer (PSEN1 or PSEN2), nicastrin (NCSTN), APH1 (APH1A or APH1B) and PEN2. Such minimal complex is sufficient for secretase activity, although other components may exist (By similarity). Interacts with PSEN1 and PSEN2.
SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein (Potential).
TISSUE SPECIFICTY: Weakly or not expressed in leukocytes, lung, placenta, small intestine, liver, kidney, spleen thymus, colon, skeletal muscle, heart and brain.
SEQUENCE SIMILARITIES: Belongs to the APH-1 family.
Molecular Weight
~31 kDa observed.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance
Evaluated by Western Blot in rat liver tissue lysate.
Western Blot Analysis: 2 µg/mL of this antibody detected NET55 on 10 µg of rat liver tissue lysate. Note: Western blot performed without Tween.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Specific nuclear envelope transmembrane proteins can promote the location of chromosomes to and from the nuclear periphery. Zuleger, N; Boyle, S; Kelly, DA; de las Heras, JI; Lazou, V; Korfali, N; Batrakou, DG; Randles, KN; Morris, GE; Harrison, DJ; Bickmore, WA; Schirmer, EC Genome biology
14
R14
2013
Different cell types have distinctive patterns of chromosome positioning in the nucleus. Although ectopic affinity-tethering of specific loci can be used to relocate chromosomes to the nuclear periphery, endogenous nuclear envelope proteins that control such a mechanism in mammalian cells have yet to be widely identified.To search for such proteins, 23 nuclear envelope transmembrane proteins were screened for their ability to promote peripheral localization of human chromosomes in HT1080 fibroblasts. Five of these proteins had strong effects on chromosome 5, but individual proteins affected different subsets of chromosomes. The repositioning effects were reversible and the proteins with effects all exhibited highly tissue-restricted patterns of expression. Depletion of two nuclear envelope transmembrane proteins that were preferentially expressed in liver each reduced the normal peripheral positioning of chromosome 5 in liver cells.The discovery of nuclear envelope transmembrane proteins that can modulate chromosome position and have restricted patterns of expression may enable dissection of the functional relevance of tissue-specific patterns of radial chromosome positioning.
