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MABS1688 Anti-NCoA-2 Antibody, clone 1C6.3

Detect NCOA2 using this mouse monoclonal Anti-NCoA-2 Antibody, clone 1C6.3, Cat. No. MABS1688, validated for use in Immunohistochemistry (Paraffin) and Western blotting.

Anti-NCoA-2 Antibody, clone 1C6.3: SDB (Sicherheitsdatenblätter), Analysenzertifikate und Qualitätszertifikate, Dossiers, Broschüren und andere verfügbare Dokumente.
Research Category: Signaling Gene Symbol: NCOA2, BHLHE75, SRC2, TIF2 UniProt Number: Q15596
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MABS1688
100 μg  
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      Übersicht

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HIH(P), WBMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABS1688
      DescriptionAnti-NCoA-2 Antibody, clone 1C6.3
      Alternate Names
      • Nuclear receptor coactivator 2
      • bHLHe75
      • Class E basic helix-loop-helix protein 75
      • hTIF2
      • NCoA-2
      • Transcriptional intermediary factor 2
      Background InformationNuclear receptor coactivator 2 (UniProt Q15596; also known as bHLHe75, Class E basic helix-loop-helix protein 75, hTIF2, NCoA-2, Transcriptional intermediary factor 2) is encoded by the NCOA2 (also known as BHLHE75, SRC2, TIF2) gene (Gene ID 10499) in human. Nuclear receptor coactivator 2 (NCoA-2) is a 160 kDa transcriptional coactivator for steroid receptors and nuclear receptors that acts as a coactivator of the steroid binding domain (AF-2), but not the modulating N-terminal domain (AF-1). It possesses a single NR interaction domain (NID) and two autonomous activation domains, AD1 and AD2. NCoA-2 assists nuclear receptors in the upregulation of DNA expression. In cells ligand activated nuclear receptors recruit NCoA-2 to DNA where it acylates histones to make targeted regions accessible to transcriptional machinery. Along with NCOA1 it controls energy balance between white and brown adipose tissues and also acts as a critical regulator of glucose metabolism. It is also involved in the positive regulation of the transcriptional activity of the glucocorticoid receptor NR3C1 by sumoylation enhancer RWDD3. NCoA-2 is reported to positively regulate the circadian clock by acting as a transcriptional coactivator for the CLOCK-ARNTL/BMAL1 heterodimer. Chromosomal aberrations involving NCOA2 may be a cause of acute myeloid leukemias.

      Ref.: Voegel, JJ et al. (1998). EMBO J. 17, 507 519.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationDetect NCOA2 using this mouse monoclonal Anti-NCoA-2 Antibody, clone 1C6.3, Cat. No. MABS1688, validated for use in Immunohistochemistry (Paraffin) and Western blotting.
      Key Applications
      • Immunohistochemistry (Paraffin)
      • Western Blotting
      Application NotesImmunohistochemistry Analysis: A 1:50 dilution from a representative lot detected nuclear NCoA-2 immunoreactivity in human lymphoma, ovarian cancer, and small intestine tissue sections.
      Biological Information
      ImmunogenGST-tagged recombinant human NCoA-2 C-terminal region fragment..
      Clone1C6.3
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone 1C6.3 targeted epitope lies within the C-terminal one fourth region of the full-length human NCoA-2.
      IsotypeIgG2aκ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • NCOA2
      • BHLHE75
      • SRC2
      • TIF2
      Purification MethodProtein G purified.
      UniProt Number
      Molecular Weight~160 kDa observed. 159.0 kDa (Met1 removed) calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in human osteosarcoma cell lysate.

      Western Blotting Analysis: 1 µg/mL of this antibody detected NCoA-2 in 10 µg of U2OS human osteosarcoma cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Bestellnummer GTIN
      MABS1688 04054839132940