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475985 Anti-Myosin, Smooth Muscle Heavy Chain Mouse mAb (SMMS-1)

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      Übersicht

      Replacement Information

      Key Spec Table

      Host
      M
      Description
      OverviewRecognizes the ~205 kDa myosin heavy chain-1 and the ~200 kDa myosin heavy chain-2 in myoepithelial cell layers in benign and in situ malignant breast lesions.
      Catalogue Number475985
      Brand Family Calbiochem®
      References
      ReferencesBabu, G.J., et al. 2000. Microsc. Res. Tech. 15, 532. Watchko, J.F., et al. 1998. Comp. Biochem. Physiol. B. Biochem. Mol. Biol. 119, 459. Small, J.V. 1995. Bioessays 17, 785. Price, R.J., et al. 1994. Circ. Res. 75, 520. Frid, M.G., et al. 1992. Dev. Biol. 153, 185. Glukhova, M.A., et al. 1990. J. Biol. Chem. 265, 13042.
      Product Information
      FormLiquid
      FormulationIn PBS, 0.2% BSA, pH 7.4.
      Positive controlMyoepithelial cell layers in benign and malignant breast tumors
      Preservative≤0.1% sodium azide
      Applications
      Key Applications Paraffin Sections
      Application NotesParaffin Sections (1-2 µg/ml, trypsin and heat pre-treatment required)
      Application CommentsStaining of formalin-fixed tissues requires digesting sections with trypsin at 1 mg/ml PBS for 10 min at 37°C, followed by boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min. Variables associated with assay conditions will dictate the proper working dilution.
      Biological Information
      Immunogena crude extract from human uterus
      ImmunogenHuman
      CloneSMMS-1
      HostMouse
      IsotypeIgG₁
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Do not freeze Yes
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Bestellnummer GTIN
      475985 0

      Documentation

      Literatur

      Übersicht
      Babu, G.J., et al. 2000. Microsc. Res. Tech. 15, 532. Watchko, J.F., et al. 1998. Comp. Biochem. Physiol. B. Biochem. Mol. Biol. 119, 459. Small, J.V. 1995. Bioessays 17, 785. Price, R.J., et al. 1994. Circ. Res. 75, 520. Frid, M.G., et al. 1992. Dev. Biol. 153, 185. Glukhova, M.A., et al. 1990. J. Biol. Chem. 265, 13042.
      Datenblatt

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision07-August-2007 RFH
      ApplicationParaffin Sections (1-2 µg/ml, trypsin and heat pre-treatment required)
      DescriptionProtein G purified mouse monoclonal antibody. Recognizes the ~205 kDa myosin heavy chain-1 and the ~200 kDa myosin heavy chain-2.
      BackgroundSmooth muscle is an important element of the vascular, digestive, urogenital and respiratory systems. Smooth muscle cells (SMCs) are spindle-shaped cells that contain a variety of obliquely arrayed actomyosin-rich contractile elements that are attached to the plasma membrane. Smooth muscle heavy chain (SM-MHC) is a structural protein that is found in the cytoplasm. SM-MHC is a major component of the contractile apparatus in smooth muscle cells. Alternative splicing of the primary transcript from a single gene generates four smooth muscle myosin heavy chain isoforms. These isoforms differ both at the carboxyl terminus (SM1 and SM2 isoforms) and at the amino terminus (SM-A and SM-B isoforms). The smooth muscle myosin heavy chain isoforms are differentially expressed during smooth muscle development and in different smooth muscle cell types.
      HostMouse
      Immunogen speciesHuman
      Immunogena crude extract from human uterus
      CloneSMMS-1
      IsotypeIgG₁
      Specieshuman
      Positive controlMyoepithelial cell layers in benign and malignant breast tumors
      FormLiquid
      FormulationIn PBS, 0.2% BSA, pH 7.4.
      Concentration Label Please refer to vial label for lot-specific concentration
      Preservative≤0.1% sodium azide
      CommentsStaining of formalin-fixed tissues requires digesting sections with trypsin at 1 mg/ml PBS for 10 min at 37°C, followed by boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min. Variables associated with assay conditions will dictate the proper working dilution.
      Storage +2°C to +8°C
      Do Not Freeze Yes
      Toxicity Standard Handling
      ReferencesBabu, G.J., et al. 2000. Microsc. Res. Tech. 15, 532. Watchko, J.F., et al. 1998. Comp. Biochem. Physiol. B. Biochem. Mol. Biol. 119, 459. Small, J.V. 1995. Bioessays 17, 785. Price, R.J., et al. 1994. Circ. Res. 75, 520. Frid, M.G., et al. 1992. Dev. Biol. 153, 185. Glukhova, M.A., et al. 1990. J. Biol. Chem. 265, 13042.