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Anti-Microphthalmia (Mi) Antibody, clone C5 is a Mouse Monoclonal Antibody for detection of Microphthalmia (Mi) also known as Microphthalmia-associated transcription factor & has been tested in EMSA, IHC, IHC(P), IP & WB.
More>>Anti-Microphthalmia (Mi) Antibody, clone C5 is a Mouse Monoclonal Antibody for detection of Microphthalmia (Mi) also known as Microphthalmia-associated transcription factor & has been tested in EMSA, IHC, IHC(P), IP & WB. Less<<
Anti-Microphthalmia (Mi) Antibody, clone C5: SDB (Sicherheitsdatenblätter), Analysenzertifikate und Qualitätszertifikate, Dossiers, Broschüren und andere verfügbare Dokumente.
MiTF (Microphthalmia associated transcription factor) is a basic helix loop helix leucine zipper (b HLH ZIP) transcription factor implicated in pigmentation, mast cells and bone development. Mutations in MiTF cause auditory pigmentary syndromes, such as Waardenburg syndrome type II, type IIa and Tietz syndrome in humans. There are two known isoforms of MiTF differing by 66 amino acids at the NH2 terminus. Shorter forms are expressed in melanocytes and run as two bands at 52 kDa and 56 kDa, while the longer Mi form runs as a cluster of bands at 60-70 kDa in osteoclasts and in B16 melonoma cells (but not other melanoma cell lines), as well as mast cells and heart. MiTF plays a critical role in the differentiation of various cell types as neural crest-derived melanocytes, mast cells, osteoclasts and optic cup-derived retinal pigment epithelium. Mi is a basic helix-loop-helix-leucin zipper (b-HLH-ZIP) transtripotion factor implicated in pigmentation, mast cells and bone development. The mutation of Mi causes Waardenburg Syndrome type II in humans. In mice, a profound loss of pigmented cells in the skin eye and inner ear results, as well as osteopetrosis and defects in natural killer and mast cells. These melanocyte isoforms have been shown by two dimensional tryptic mapping to differ in c-Kit-induced phosphorylation. Osteopetrotic rat strain harbors a large genomic deletion encompassing the 3' half of Mi including most of the b-HLH-ZIP region. Osteoclasts from these animals lack Mi protein in contrast to wild-type rat, mouse, and human osteoclasts.
References
Product Information
Format
Purified
HS Code
3002 15 90
Control
501 Mel human melanoma cells, wild-type human, rat, mouse osteoclast cells.
Presentation
Purified mouse monoclonal IgG1 in phosphate buffered saline with 0.08% sodium azide.
Anti-Microphthalmia (Mi) Antibody, clone C5 is a Mouse Monoclonal Antibody for detection of Microphthalmia (Mi) also known as Microphthalmia-associated transcription factor & has been tested in EMSA, IHC, IHC(P), IP & WB.
Key Applications
Electrophoretic Mobility Shift Assay
Immunohistochemistry
Immunohistochemistry (Paraffin)
Immunoprecipitation
Western Blotting
Application Notes
Immunoprecipitation: A previous lot of this antibody was used in IP. (2 µg/mg of protein lysate)
Gel supershift assays: A previous lot of this antibody was used in EMSA.
Immunohistochemistry(paraffin): A previous lot of this antibody was used in IH on frozen and formalin/paraffin tissue sections.
Optimal working dilutions must be determined by end user.
Biological Information
Immunogen
Hybridoma produced by the fusion of splenocytes from RBF/DnJ mice immunized with an N-terminal fragment of human microphthalmia protein and mouse myeloma NS1 cells.
Clone
C5
Concentration
Please refer to the Certificate of Analysis for the lot-specific concentration.
Host
Mouse
Specificity
In Western blotting, it recognizes a doublet of 52-56kDa, identified as serine-phosphorylated and unphosphorylated forms of melanocytic isoforms of microphthalmia (Mi). There are two known isoforms of Mi differing by 66 amino acids at the NH2 terminus. Shorter forms are expressed in melanocytes and run as two bands at 52kDa and 56kDa, while the longer Mi form runs as a cluster of bands at 60-70kDa in osteoclasts and in B16 melonoma cells (but not other melanoma cell lines), as well as mast cells andheart. It reacts with both melanocytic as well as the nonmelanocytic isoforms of Mi. This Ab does not cross-react with other b-HLH-ZIP factors by DNA mobility shift assay.
Isotype
IgG1
Species Reactivity
Human
Rat
Mouse
Species Reactivity Note
Expected to react with human and rat based on sequence homology.
Antibody Type
Monoclonal Antibody
Gene Symbol
LZMS
MCOPS1
MAA
Purification Method
Protein A Purfied
Molecular Weight
53-56 kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance
Routinely evaluated by Western Blot on Mouse Brain lysates.
Western Blot Analysis: 1:500 dilution of this lot detected Mi on 10 μg of Mouse Brain lysates.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 1 year at -20ºC in undiluted aliquots from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage the IgG1 and affect product performance.
Partially purified components of Nardostachys chinensis suppress melanin synthesis through ERK and Akt signaling pathway with cAMP down-regulation in B16F10 cells. Ji Yeon Jang,Ha Neui Kim,Yu Ri Kim,Woo Young Choi,Yung Hyun Choi,Hwa Kyoung Shin,Byung Tae Choi Journal of ethnopharmacology
137
2010
Ethnopharmacological relevance Nardostachys chinensis has been used in folk medicine to treat melasma and lentigines in Korea. We investigated the inhibitory activities of Nardostachys chinensis in melanogenesis and its related signaling pathway.
Inhibitory effects of 1-O-methyl-fructofuranose from Schisandra chinensis fruit on melanogenesis in B16F0 melanoma cells. Eun Young Oh,Ji Yeon Jang,Yung Hyun Choi,Young Whan Choi,Byung Tae Choi Journal of ethnopharmacology
132
2009
1-O-methyl-fructofuranose (1-O-MFF) from the fruit of Schisandra chinensis is a traditional Korean medicinal herb that has a variety of beneficial properties. The effect of purified 1-O-MFF on melanogenesis including the activation of related signaling pathways was investigated.
Germline mutations at loci encoding the transcription factor Microphthalmia (Mi), the cytokine receptor c-Kit, or its ligand Steel factor (S1) result in strikingly similar defects in mast cell and melanocyte development. Here we describe a biochemical link between Kit signalling and the activity of Mi. Stimulation of melanoma cells with S1 results in activation of MAP kinase, which in turn phosphorylates Mi at a consensus target serine. This phosphorylation upregulates Mi transactivation of the tyrosinase pigmentation gene promoter. In addition to modulating pigment production, such signalling may regulate the expression of genes essential for melanocyte survival and development. The pathway represents a new application of the general MAP kinase machinery in transducing a signal between a tissue-specific receptor at the cell surface and a tissue-specific transcription factor in the nucleus.
