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ABN1661 Anti-Membralin

Anti-Membralin Antibody, Cat. No. ABN1661, is a highly specific rabbit polyclonal antibody that targets Membralin and has been tested in Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, and Western Blotting.

Anti-Membralin: SDB (Sicherheitsdatenblätter), Analysenzertifikate und Qualitätszertifikate, Dossiers, Broschüren und andere verfügbare Dokumente.
Research Category: Neuroscience Gene Symbol: Tmem259, ORF61 UniProt Number: Q8CIV2
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ABN1661
100 μg  
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      Übersicht

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, MWB, ICC, IHC, IPRbPurifiedPolyclonal Antibody
      Description
      Catalogue NumberABN1661
      DescriptionAnti-Membralin
      Alternate Names
      • Transmembrane protein 259
      Background InformationMembralin (UniProt: Q8CIV2; also known as Transmembrane protein 259) is encoded by the Tmem259 (also known as ORF61) gene (Gene ID: 216157) in murine species. Membralin is a highly conserved protein with several transmembrane regions. However, the sequence of membralin does not show any closely related genes. It has multiple glycosylation sites and cytosolic regions at both the N-terminal ends and C-terminal ends. It is expressed in multiple tissues, including brain and spinal cord. However, it is not found in heart, skeletal muscle, and spleen. In humans its membralin expression is reported in certain cancers, including the ovarian cancers. Membralin is reported to have a role in the ER-associated protein degradation (ERAD) pathway and is required for clearance of misfolded proteins in the endoplasmic reticulum (ER). It is also required for survival of motor neurons. Membralin mutant mice display increased basal ER stress and severe and early-onset motor neuron disease and die by postnatal day 5 or 6. (Ref.: Chen YC et al. (2005). Biochimica Biophysica Acta 1730(2): 96-102.)
      References
      Product Information
      FormatPurified
      PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Membralin Antibody, Cat. No. ABN1661, is a highly specific rabbit polyclonal antibody that targets Membralin and has been tested in Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, and Western Blotting.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      • Immunohistochemistry
      • Immunoprecipitation
      Application NotesImmunoprecipitation Analysis: 1.0 µg/mL from a representative lot detected Membralin in lysate from HEK293 cells transfected with mouse membralin. (Courtesy of Dr. Lu-Lin Jiang and Dr. Bing Zhu in Dr. Dongxian Zhang's Lab at Sanford Burnham Prebys Medical Discovery Institute).

      Immunocytochemistry Analysis: 10 µg/mL from a representative lot detected Membralin in mouse neural progenitor cells. (Courtesy of Dr. Lu-Lin Jiang and Dr. Bing Zhu in Dr. Dongxian Zhang's Lab at Sanford Burnham Prebys Medical Discovery Institute).

      Immunohistochemistry Analysis: 5.0 µg/mL from a representative lot detected Membralin in mouse spinal cord tissues. (Courtesy of Dr. Lu-Lin Jiang and Dr. Bing Zhu in Dr. Dongxian Zhang's Lab at Sanford Burnham Prebys Medical Discovery Institute).

      Western Blotting Analysis: 1.0 µg/mL from a representative lot detected Membralin in lysate from HEK293 cells transfected with mouse membralin. (Courtesy of Dr. Lu-Lin Jiang and Dr. Bing Zhu in Dr. Dongxian Zhang's Lab at Sanford Burnham Prebys Medical Discovery Institute).
      Biological Information
      ImmunogenA Myc-conjugated linear peptide from the N-terminal region of mouse membralin.
      EpitopeN-terminus
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityThis polyclonal antibody specifically detects membralin in HEK293 cells overexpressing membralin.
      Species Reactivity
      • Human
      • Mouse
      Species Reactivity NoteHuman, Mouse.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Tmem259
      • ORF61
      Purification MethodProtein A + Protein G
      UniProt Number
      Molecular Weight~68 kDa observed; 63.56 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HEK293 cells overexpressing membralin.

      Western Blotting Analysis: 1 µg/mL of this antibody detected Membralin in 4 µL of lysate from HEK293 cells transfected with mouse-myc-membralin and overexpressing Membralin.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Bestellnummer GTIN
      ABN1661 04054839169571