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Anti-MYO18A, clone DY12, Cat. No. MABT1319, is a mouse monoclonal antibody that detects Unconventional myosin 18A and is tested for use in and Agonist function Analysis, Flow Cytometry, Immunohistochemistry, Immunoprecipitation, and Western Blotting.
More>>Anti-MYO18A, clone DY12, Cat. No. MABT1319, is a mouse monoclonal antibody that detects Unconventional myosin 18A and is tested for use in and Agonist function Analysis, Flow Cytometry, Immunohistochemistry, Immunoprecipitation, and Western Blotting. Less<<
Unconventional myosin-18A (UniProt: Q92614; also known as Molecule associated with JAK3 N-terminus, MAJN, Myosin containing a PDZ domain, Surfactant protein receptor SP-R210, SP-R210, CD245) is encoded by the MYO18A (also known as CD245, KIAA0216, MYSPDZ) gene (Gene ID: 399687) in human. CD245 is an unconventional myosin 18A that serves as a highly conserved motor enzyme and acts as a receptor for the surfactant protein A (SP-A). It plays a critical role in cytoskeleton organization and Golgi budding. It has a distinctive N-terminal extension, which includes a highly charged lysine-glutamine- rich region and a PDZ domain (aa 220-311). Its shorter splice variant lacks the N-terminal extension. CD245 is constitutively expressed in most lymphocytes and its membrane expression is enhanced on NK, T and B lymphocytes following activation. Monocytes also express CD245, but at higher levels than lymphocytes. It regulates trafficking, expression, and activation of innate immune receptors on macrophages and suppresses inflammatory responsiveness of macrophages. CD245 serves as a potent activator of NK cell co-activating receptor. It interacts with PAK-2 and SHP-2, the two key signal transducers of the NK cell activation and degranulation processes. The recruitment of CD245 is shown to strongly enhance NK cell cytotoxicity. Deletion of MYO18A gene in mice shown to be embryonically lethal. CD245 stimulation by this antibody (Clone DY12) is shown to increase NK cell degranulation and lymphokine-activated killer activity toward tumor B cells and EBV-infected B cells, respectively. (Ref.: De Masson, A., et al. (2016). Oncoimmunology 5(5); e1127493; Horsthemke. M., et al. (2019). J. Biol. Chem. 294(18); 7202-7218).
References
Product Information
Format
Purified
Presentation
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Applications
Application
Anti-MYO18A, clone DY12, Cat. No. MABT1319, is a mouse monoclonal antibody that detects Unconventional myosin 18A and is tested for use in and Agonist function Analysis, Flow Cytometry, Immunohistochemistry, Immunoprecipitation, and Western Blotting.
Key Applications
Affects Function
Flow Cytometry
Immunohistochemistry
Immunoprecipitation
Western Blotting
Application Notes
Western Blotting Analysis: A representative lot detected Myosin 18A (MYO18A) in Western Blotting applications.
Immunoprecipitation Analysis: A representative lot immunoprecipitated MYO18A in Immunoprecipitation applications (De Masson, A., et. al. (2016). Oncoimmunology. 5(5):e1127493).
Agonist Analysis: A representative lot caused activation of NK cells in Agonist function analysis .De Masson, A., et. al. (2016). Oncoimmunology. 5(5):e1127493).
Flow Cytometry Analysis: A representative lot detected MYO18A in Flow Cytometry applications (De Masson, A., et. al. (2016). Oncoimmunology. 5(5):e1127493).
Immunohistochemistry Analysis: A representative lot detected MYO18A in Immunohistochemistry applications (De Masson, A., et. al. (2016). Oncoimmunology. 5(5):e1127493).
Flow Cytometry Analysis: A 1:500 dilution from a representative lot detected MYO18A in MyLa cells (Courtesy of Armand Bensussan, PhD, Head of INSERM HIPI Unit (Human Immunology, Pathophysiology and Immunotherapy), Hôpital Saint Louis, Paris, France).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Biological Information
Immunogen
Human NK-like cell line YT2C2.
Clone
DY12
Concentration
0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Host
Mouse
Specificity
Clone DY12 is a mouse monoclonal antibody that detects Unconventional myosin-18A (MYO18A).
Isotype
IgG1κ
Species Reactivity
Human
Species Reactivity Note
Human. Predicted to react with Baboon based on 100% sequence homology.
Isotype testing: Identity Confirmation by Isotyping Test.
Isotyping Analysis: The identity of this monoclonal antibody is confirmed by isotyping test to be mouse IgG1.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Identification of CD245 as myosin 18A, a receptor for surfactant A: A novel pathway for activating human NK lymphocytes. De Masson A, Giustiniani J, Marie-Cardine A, Bouaziz JD, Dulphy N, Gossot D, Validire P, Tazi A, Garbar C, Bagot M, Merrouche Y, Bensussan A. Oncoimmunology
5(5)
e1127493
2015
CD245 is a human surface antigen expressed on peripheral blood lymphocytes, initially delineated by two monoclonal antibodies DY12 and DY35. Until now, CD245 molecular and functional characteristics remained largely unknown. We combined immunological and proteomic approaches and identified CD245 as the unconventional myosin 18A, a highly conserved motor enzyme reported as a receptor for the surfactant protein A (SP-A), that plays a critical role in cytoskeleton organization and Golgi budding. We report that the recruitment of CD245 strongly enhanced NK cell cytotoxicity. Further, we show that the enhancement of the NK lymphocytes killing ability toward CD137-ligand expressing target cells could result from the induction of CD137 expression following CD245 engagement. The SP-A receptor could therefore represent a novel and promising target in cancer immunotherapy.
