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ST1652 Anti-MVK Mouse mAb (2C5)

Anti-MVK Mouse mAb (2C5): SDB (Sicherheitsdatenblätter), Analysenzertifikate und Qualitätszertifikate, Dossiers, Broschüren und andere verfügbare Dokumente.

Synonyme: Anti-Mevalonate Kinase

ST1652
  
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      Übersicht

      Replacement Information

      Key Spec Table

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      M
      Description
      Overview

      This product has been discontinued.





      Recognizes the MVK protein in human salivary gland tissue.
      Catalogue NumberST1652
      Brand Family Calbiochem®
      SynonymsAnti-Mevalonate Kinase
      Application Data
      Detection of human MVK by immunoblotting. Sample: Whole cell lysate from 293T cells transfected with partial recombinant MVK (lane 1) and from non-transfected 293T cells (lane 2). Primary antibody: Anti-MVK Mouse mAb (2C5) (Cat. No. ST1652) (1-5 µg/ml). Detection: chemiluminescence.

      Detection of human MVK by staining paraffin sections. Sample: Formalin-fixed, paraffin-embedded human salivary gland tissue. Primary antibody: Anti-MVK Mouse mAb (2C5) (Cat. No. ST1652) (3 µg/ml). Detection: colorimetric (DAB).
      References
      Product Information
      FormLiquid
      FormulationIn PBS, pH 7.2.
      Negative control293T cells
      Positive controlHuman salivary gland tissue
      PreservativeNone
      Applications
      Key Applications Enzyme-Linked Immunosorbent Assay
      Immunoblotting (Western Blotting)
      Paraffin Sections
      Application NotesELISA (see comments)
      Immunoblotting (1-5 µg/ml)
      Paraffin Sections (3 µg/ml)
      Application CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
      Biological Information
      ImmunogenA recombinant polypeptide corresponding to amino acids of 297-397 human MVK, expressed as a GST fusion protein
      Clone2C5
      HostMouse
      IsotypeIgG2a
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Bestellnummer GTIN
      ST1652 0

      Documentation

      Datenblatt

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision10-November-2010 RFH
      SynonymsAnti-Mevalonate Kinase
      ApplicationELISA (see comments)
      Immunoblotting (1-5 µg/ml)
      Paraffin Sections (3 µg/ml)
      Application Data
      Detection of human MVK by immunoblotting. Sample: Whole cell lysate from 293T cells transfected with partial recombinant MVK (lane 1) and from non-transfected 293T cells (lane 2). Primary antibody: Anti-MVK Mouse mAb (2C5) (Cat. No. ST1652) (1-5 µg/ml). Detection: chemiluminescence.

      Detection of human MVK by staining paraffin sections. Sample: Formalin-fixed, paraffin-embedded human salivary gland tissue. Primary antibody: Anti-MVK Mouse mAb (2C5) (Cat. No. ST1652) (3 µg/ml). Detection: colorimetric (DAB).
      DescriptionPurified mouse monoclonal antibody. Recognizes the MVK protein.
      BackgroundThis gene encodes the peroxisomal enzyme mevalonate kinase. Mevalonate is a key intermediate, and mevalonate kinase a key early enzyme, in isoprenoid and sterol synthesis. Mevalonate kinase deficiency caused by mutation of this gene results in mevalonic aciduria, a disease characterized psychomotor retardation, failure to thrive, hepatosplenomegaly, anemia and recurrent febrile crises. Defects in this gene also cause hyperimmunoglobulinaemia D and periodic fever syndrome, a disorder characterized by recurrent episodes of fever associated with lymphadenopathy, arthralgia, gastrointestinal dismay and skin rash. Two transcript variants that encode the same protein have been found for this gene.
      HostMouse
      ImmunogenA recombinant polypeptide corresponding to amino acids of 297-397 human MVK, expressed as a GST fusion protein
      Clone2C5
      IsotypeIgG2a
      Specieshuman
      Positive controlHuman salivary gland tissue
      Negative control293T cells
      FormLiquid
      FormulationIn PBS, pH 7.2.
      PreservativeNone
      CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Standard Handling