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Anti-MORC3, clone 17A9, Cat. No. MABC1105, is a mouse monoclonal antibody that detects MORC family CW-type zinc finger protein 3 and is tested for use in Immunocytochemistry and Western Blotting.
More>>Anti-MORC3, clone 17A9, Cat. No. MABC1105, is a mouse monoclonal antibody that detects MORC family CW-type zinc finger protein 3 and is tested for use in Immunocytochemistry and Western Blotting. Less<<
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Übersicht
Replacement Information
Description
Catalogue Number
MABC1105-100UG
Description
Anti-MORC3 Antibody, clone 17A9
Alternate Names
MORC family CW-type zinc finger protein 3
Nuclear matrix protein 2
Zinc finger CW-type coiled-coil domain protein 3
Background Information
MORC family CW-type zinc finger protein 3 (UniProt: Q14149; also known as Nuclear matrix protein 2, Zinc finger CW-type coiled-coil domain protein 3) is encoded by the MORC3 (also known as KIAA0136, NXP2, ZCWCC3) gene (Gene ID: 23515) in human. MORC3 is a homodimeric protein of the MORC protein family characterized by conserved structures consisting of Gyrase H, Hsp90, and MutL (GHL)-ATPase domain at its N-terminus and a CW type zinc finger domain (aa 404-454). It is expressed in heart, placenta, skeletal muscle, brain, pancreas, lung, and liver. MORC3 serves as a nuclear factor that forms MORC3-NBs (nuclear bodies) via an ATP-dependent mechanism. It is also shown to be a target of herpes simplelx virus 1 (HSV-1) ICP0-mediated degradation. It is shown to be essential for fully efficient recruitment of PML, Sp100, hDaxx, and H2AX to sites associated with HSV-1 genomes entering the host cell nucleus. MORC3 recruits TP53 and SP100 to PML-NBs, hence, it is also involved in the regulation of TP53 activity. It regulates p53 activation via MORC3-ATPase activity. It can also induce p53-dependent premature senescence. (Ref.: Takahashi, K., et al. (2007). Mol Biol Cell. 18(5): 1701 1709; Sloan, E., et al. (2016). J. virology. 90(19); 8621-8633).
References
Product Information
Format
Purified
Presentation
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Applications
Application
Anti-MORC3, clone 17A9, Cat. No. MABC1105, is a mouse monoclonal antibody that detects MORC family CW-type zinc finger protein 3 and is tested for use in Immunocytochemistry and Western Blotting.
Key Applications
Immunocytochemistry
Western Blotting
Application Notes
Immunocytochemistry Analysis: A representative lot detected MORC3 in Immunocytochemistry applications (Takahashi, K., et. al. (2007). Mol Biol Cell. 18(5):1701-9).
Western Blotting Analysis: A 1:250 dilution from a representative lot detected MORC3 in PC12 cell lysates.
Biological Information
Immunogen
His-tagged full-length recombinant human MORC family CW-type zinc finger protein 3 (MORC3).
Clone
17A9
Concentration
Please refer to lot specific datasheet.
Host
Mouse
Specificity
Clone 17A9 is a mouse monoclonal antibody that specifically detects MORC family CW-type zinc finger protein 3 (MORC3). It targets an epitope with the C-terminal region.
~ 160 and 230 kDa observed; 107.11 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance
Evaluated by Western Blotting in NIH/3T3 cell lysates.
Western Blotting Analysis: 1 µg/mL of this antibody detected MORC3 in NIH/3T3 cell lysates.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
The tumor suppressor p53 is a key transcriptional factor regulating the induction of cellular senescence by oncogenic signals. The activity of p53 is regulated by recruitment into promyelocytic leukemia (PML)-nuclear bodies (NBs) as well as by stabilization through posttranslational modifications such as phosphorylation and acetylation. Here we found that MORC3 (microrchidia3)-ATPase activated p53 and induced cellular senescence in normal human and mouse fibroblasts but not p53-/- fibroblasts. Conversely, genotoxic stress-induced phosphorylation and stabilization of p53 but barely increased its transcriptional activity in Morc3-/- fibroblasts. MORC3 localized on PML-NBs in presence of PML and mediated recruitment of p53 and CREB-binding protein (CBP) into PML-NBs. In contrast, expression of ATPase activity-deficient mutant MORC3-E35A or siRNA repression of MORC3 impaired the localization of p53 and Sp100 but not CBP on PML-NBs. These results suggest that MORC3 regulates p53 activity and localization into PML-NBs. We identified a new molecular mechanism that regulates the activity of nuclear proteins by localization to a nuclear subdomain.
