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MABT531 Anti-MMP-9 Antibody, clone 3D6.3

This mouse monoclonal Anti-MMP-9 Antibody, clone 3D6.3, Cat. No. MABT531 is tested in Immunohistochemistry (Paraffin) and Western Blotting for the detection of MMP-9.

Anti-MMP-9 Antibody, clone 3D6.3: SDB (Sicherheitsdatenblätter), Analysenzertifikate und Qualitätszertifikate, Dossiers, Broschüren und andere verfügbare Dokumente.
Research Category: Cell Structure Gene Symbol: MMP9, CLG4B UniProt Number: P14780
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MABT531
100 μg  
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      Übersicht

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HIH(P), WBMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABT531
      ReplacesMAB3309
      DescriptionAnti-MMP-9 Antibody, clone 3D6.3
      Alternate Names
      • Matrix metalloproteinase-9
      • 92 kDa gelatinase
      • 92 kDa type IV collagenase
      • Gelatinase B
      • GELB
      Background InformationMatrix metalloproteinase-9 (UniProt: P14780; also known as MMP-9, 92 kDa gelatinase, 92 kDa type IV collagenase, Gelatinase B, GELB) is encoded by the MMP9 (also known as CLG4B) gene (Gene ID: 4318) in human. MMP-9 is synthesized as inactive proenzyme with a 19 amino acids signal peptide and a 73 amino acids propeptide that are cleaved to generate the active enzyme. MMP-9 is produced by normal alveolar macrophages and granulocytes. It is zinc-binding enzyme that binds two zinc ions per subunit. It can be present as a monomer or a disulfide-linked homodimer. Macrophages and transformed cells usually produce only the monomeric form. MMP-9 contains a conserved cysteine that is present in the cysteine-switch motif and binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme. MMP-9 plays an essential role in local proteolysis of the extracellular matrix, in leukocyte migration, and bone osteoclastic resorption. It cleaves type IV and type V collagen into large C-terminal three quarter fragments and shorter N-terminal one quarter fragments. MMP-9 expression is regulated by several cytokines and growth factors that induce the expression and/or activation of c-Fos and c-Jun proto-oncogene products, which heterodimerize and bind activator protein-1 (AP-1) sites within of MMP-9 gene promoters. In coordination with other matrix metalloproteinases, it plays a role in normal tissue remodeling events, including neurite growth, embryonic development, angiogenesis, ovulation, mammary gland involution and wound healing. Abnormalities in MMP-9 are associated with a variety of autoimmune diseases, including systemic lupus erythematosus, Sjogren's syndrome, systemic sclerosis, rheumatoid arthritis, multiple sclerosis, polymyositis and atherosclerosis. Mutations in MMP9 gene are known to cause intervertebral disc disease (IDD) and metaphyseal anadysplasia that are characterized by various skeletal abnormalities.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationThis mouse monoclonal Anti-MMP-9 Antibody, clone 3D6.3, Cat. No. MABT531 is tested in Immunohistochemistry (Paraffin) and Western Blotting for the detection of MMP-9.
      Key Applications
      • Immunohistochemistry (Paraffin)
      • Western Blotting
      Application NotesImmunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected MMP-9 in human bone marrow and human tonsil tissues.
      Biological Information
      ImmunogenGST/His-tagged recombinant fragment corresponding to 110 amino acids from the C-terminal of human MMP-9.
      Clone3D6.3
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone 3D6.3 detects MMP-9 in human spleen. This clone targets a sequence with in 110 amino acids from the C-terminal end.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • MMP9
      • CLG4B
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~92 kDa observed; 78.46 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in human spleen tissue lysate.

      Western Blotting Analysis: 0.5 µg/mL of this antibody detected MMP-9 in 10 µg of human spleen tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Bestellnummer GTIN
      MABT531 04054839168260