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IM09L
Sigma-AldrichAnti-MMP-9 (Ab-1) Mouse mAb (6-6B)
This Anti-MMP-9 (Ab-1) Mouse mAb (6-6B) is validated for use in Immunoblotting, Immunoprecipitation, Neutralization Studies, Paraffin Sections for the detection of MMP-9 (Ab-1).
More>>This Anti-MMP-9 (Ab-1) Mouse mAb (6-6B) is validated for use in Immunoblotting, Immunoprecipitation, Neutralization Studies, Paraffin Sections for the detection of MMP-9 (Ab-1). Less<<
Anti-MMP-9 (Ab-1) Mouse mAb (6-6B): SDB (Sicherheitsdatenblätter), Analysenzertifikate und Qualitätszertifikate, Dossiers, Broschüren und andere verfügbare Dokumente.
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Description
Overview
Recognizes the ~92 kDa latent and the ~83 kDa active forms of MMP-9 under non-reducing conditions, but only the latent form under reducing conditions. Inhibits the enzymatic activity of MMP-9. For paraffin sections, use Cat. No. IM37L.
Cottam, D.W. and Rees, R.C. 1993. Intl. J. of Oncol.2, 861. Nakajima, M., et al. 1993. Cancer Res.53, 5802. Ramos-DeSimone, et al. 1993. Hybridoma. 12, 349. Stetler-Stevenson, W.G., et al. 1993. FASEB.7, 1434. Zucker, S., et al. 1993. Cancer Res.53, 140. Woessner, J.F. 1991. FASEB. 5, 2145. Liotta, L.A. and Stetler-Stevenson, W.G. 1990. in Seminars in Cancer Biology, ed. M.M. Gottesman. Vol. 1; 99-106.
Immunoprecipitation
Ramos-DeSimone, N. and French, D. L. 1994. Ann. NY Acad. Sci.732, 469.
Immunoblotting, Neutralization Studies
Ramos-DeSimone, N., et al. 1993. Hybridoma.12, 349.
Key Applications
Immunoblotting (Western Blotting)
Immunoprecipitation
Not Paraffin Sections
Neutralization Studies
Application Notes
Immunoblotting (2 µg/ml) Immunoprecipitation (see appplication references) Neutralization Studies (see comments and application references) Paraffin Sections (not recommended)
Application Comments
Inhibits MMP-9 enzymatic activity. For staining paraffin sections, use Anti-MMP-9 (626-644) (Ab-3) Mouse mAb (56-2A4) (Cat. No. IM37L). Antibody should be titrated for optimal results in individual systems.
Biological Information
Immunogen
MMP-9 from conditioned medium of PMA-stimulated HT-1080 human fibrosarcoma cells
Immunogen
Human
Clone
6-6B
Host
Mouse
Isotype
IgG₁
Species Reactivity
Human
Antibody Type
Monoclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code
Ambient Temperature Only
Toxicity
Standard Handling
Storage
+2°C to +8°C
Do not freeze
Ok to freeze
Special Instructions
Store at 4°C until reconstituted, then store in aliquots at -20°C or at 4°C with 0.1% azide. Resuspend the antibody with sterile PBS, pH 7.4, or sterile 20 mM Tris-saline, pH 7.4, to yield a final concentration of 100 µg/ml; product will be more stable if 0.1% sodium azide is included (do not add azide if antibody is to be used with viable cells). Lyophilized antibodies should be resuspended at 4°C with occasional gentle mixing for at least 2 h. Freezing of aliquots is best for long-term storage of reconstituted product; repetitive freezing and thawing should be avoided.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Bestellnummer
GTIN
IM09L-100UG
04055977228151
IM09L-1MG
04055977228144
Documentation
Anti-MMP-9 (Ab-1) Mouse mAb (6-6B) Analysenzertifikate
Titel
Chargennummer
IM09L
Literatur
Übersicht
Cottam, D.W. and Rees, R.C. 1993. Intl. J. of Oncol.2, 861. Nakajima, M., et al. 1993. Cancer Res.53, 5802. Ramos-DeSimone, et al. 1993. Hybridoma. 12, 349. Stetler-Stevenson, W.G., et al. 1993. FASEB.7, 1434. Zucker, S., et al. 1993. Cancer Res.53, 140. Woessner, J.F. 1991. FASEB. 5, 2145. Liotta, L.A. and Stetler-Stevenson, W.G. 1990. in Seminars in Cancer Biology, ed. M.M. Gottesman. Vol. 1; 99-106.
Datenblatt
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
Immunoblotting (2 µg/ml) Immunoprecipitation (see appplication references) Neutralization Studies (see comments and application references) Paraffin Sections (not recommended)
Description
Purified mouse monoclonal antibody. Recognizes the ~92 kDa latent and the ~83 kDa active forms of human MMP-9 under non-reducing conditions, but only the latent form under reducing conditions.
Background
Matrix metalloproteinases (MMP's) are a family of enzymes that are responsible for the degradation of extracellular matrix components such as collagen, laminin and proteoglycans. In addition to sequence homology, all MMP's share the following characteristics: the catalytic mechanism is dependent upon a zinc ion at the active center, they cleave one or more extracellular matrix components, they are secreted as zymogens which are activated by removal of an ~10 kDa segment from the N terminus and they are inhibited by tissue inhibitor of metalloproteinases (TIMP). These enzymes are involved in normal physiological processes such as embryogenesis and tissue remodeling and may play an important role in arthritis, periodontitis, and metastasis.
MMP-9 (Gelatinase B, 92 kDa gelatinase/type IV collagenase) is secreted as a 92 kDa zymogen which is proteolytically processed to the 83 kDa active form; a 68 kDa has also been detected. MMP-9, along with its most closely related member of the MMP family, MMP-2, show substrate specificity toward type IV and V collagens, gelatin and elastin. Numerous studies have shown a correlation between collagenase expression and metastatic potential. Elevated levels of MMP-9 in plasma suggest that it may be a useful marker for the diagnosis or prognosis of cancer in general.
Host
Mouse
Immunogen species
Human
Immunogen
MMP-9 from conditioned medium of PMA-stimulated HT-1080 human fibrosarcoma cells
Clone
6-6B
Isotype
IgG₁
Species
human
Positive control
MMP-9 protein (Cat. Nos. PF024 or PF038)
Form
Lyophilized
Formulation
Lyophilized from a volatile buffer, 100 µg BSA.
Preservative
None
Comments
Inhibits MMP-9 enzymatic activity. For staining paraffin sections, use Anti-MMP-9 (626-644) (Ab-3) Mouse mAb (56-2A4) (Cat. No. IM37L). Antibody should be titrated for optimal results in individual systems.
Storage
+2°C to +8°C
Do Not Freeze
Ok to freeze
Special Instructions
Store at 4°C until reconstituted, then store in aliquots at -20°C or at 4°C with 0.1% azide. Resuspend the antibody with sterile PBS, pH 7.4, or sterile 20 mM Tris-saline, pH 7.4, to yield a final concentration of 100 µg/ml; product will be more stable if 0.1% sodium azide is included (do not add azide if antibody is to be used with viable cells). Lyophilized antibodies should be resuspended at 4°C with occasional gentle mixing for at least 2 h. Freezing of aliquots is best for long-term storage of reconstituted product; repetitive freezing and thawing should be avoided.
Toxicity
Standard Handling
References
Cottam, D.W. and Rees, R.C. 1993. Intl. J. of Oncol.2, 861. Nakajima, M., et al. 1993. Cancer Res.53, 5802. Ramos-DeSimone, et al. 1993. Hybridoma. 12, 349. Stetler-Stevenson, W.G., et al. 1993. FASEB.7, 1434. Zucker, S., et al. 1993. Cancer Res.53, 140. Woessner, J.F. 1991. FASEB. 5, 2145. Liotta, L.A. and Stetler-Stevenson, W.G. 1990. in Seminars in Cancer Biology, ed. M.M. Gottesman. Vol. 1; 99-106.
Application references
Immunoprecipitation
Ramos-DeSimone, N. and French, D. L. 1994. Ann. NY Acad. Sci.732, 469.
Immunoblotting, Neutralization Studies
Ramos-DeSimone, N., et al. 1993. Hybridoma.12, 349.
