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DR1080 Anti-MCM2 Mouse mAb (6A8)

This Anti-MCM2 Mouse mAb (6A8) is validated for use in ELISA, Immunoblotting, Immunocytochemistry for the detection of MCM2.

Anti-MCM2 Mouse mAb (6A8): SDB (Sicherheitsdatenblätter), Analysenzertifikate und Qualitätszertifikate, Dossiers, Broschüren und andere verfügbare Dokumente.

Synonyme: Anti-Minichromosome Maintenance Complex Component 2

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DR1080
  
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      Übersicht

      Replacement Information

      Key Spec Table

      Species ReactivityHostAntibody Type
      HMMonoclonal Antibody
      Description
      OverviewRecognizes the ~140-145 kDa MCM2 protein in HeLa cells.
      Catalogue NumberDR1080
      Brand Family Calbiochem®
      SynonymsAnti-Minichromosome Maintenance Complex Component 2
      Application Data
      Detection of human MCM2 by immunoblotting. Sample: Whole cell lysate from HeLa NE cells. Primary antibody: Anti-MCM2 Mouse mAb (6A8) (Cat. No. DR1080) (1-5 µg/ml). Detection: chemiluminescence.

      Detection of human MCM2 by immunocytochemistry. Sample: HeLa cells fixed in paraformaldehyde. Primary antibody: Anti-MCM2 Mouse mAb (6A8) (Cat. No. DR1080) (10 µg/ml). Detection: fluorescence.
      References
      Product Information
      FormLiquid
      FormulationIn PBS, pH 7.2.
      Positive controlHeLa cells
      PreservativeNone
      Quality LevelMQ100
      Applications
      Key Applications Enzyme-Linked Immunosorbent Assay
      Immunoblotting (Western Blotting)
      Immunocytochemistry
      Application NotesELISA (see comments)
      Immunoblotting (1-5 µg/ml)
      Immunocytochemistry (10 µg/ml)
      Application CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
      Biological Information
      ImmunogenA recombinant polypeptide corresponding to amino acids of 805-904 human MCM2, expressed as a GST fusion protein
      Clone6A8
      HostMouse
      IsotypeIgG2a
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Regulatory Review
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Bestellnummer GTIN
      DR1080 0

      Documentation

      Anti-MCM2 Mouse mAb (6A8) SDB

      Titel

      Sicherheitsdatenblatt (SDB) 

      Datenblatt

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision04-November-2010 RFH
      SynonymsAnti-Minichromosome Maintenance Complex Component 2
      ApplicationELISA (see comments)
      Immunoblotting (1-5 µg/ml)
      Immunocytochemistry (10 µg/ml)
      Application Data
      Detection of human MCM2 by immunoblotting. Sample: Whole cell lysate from HeLa NE cells. Primary antibody: Anti-MCM2 Mouse mAb (6A8) (Cat. No. DR1080) (1-5 µg/ml). Detection: chemiluminescence.

      Detection of human MCM2 by immunocytochemistry. Sample: HeLa cells fixed in paraformaldehyde. Primary antibody: Anti-MCM2 Mouse mAb (6A8) (Cat. No. DR1080) (10 µg/ml). Detection: fluorescence.
      DescriptionPurified mouse monoclonal antibody. Recognizes the ~140-145 kDa MCM2 protein.
      BackgroundThe protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins.
      HostMouse
      ImmunogenA recombinant polypeptide corresponding to amino acids of 805-904 human MCM2, expressed as a GST fusion protein
      Clone6A8
      IsotypeIgG2a
      Specieshuman
      Positive controlHeLa cells
      FormLiquid
      FormulationIn PBS, pH 7.2.
      PreservativeNone
      CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Regulatory Review