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ST1642 Anti-MAP4K5 Mouse mAb (3H3)

Anti-MAP4K5 Mouse mAb (3H3): SDB (Sicherheitsdatenblätter), Analysenzertifikate und Qualitätszertifikate, Dossiers, Broschüren und andere verfügbare Dokumente.

Synonyme: Anti-Mitogen-Activated Protein Kinase Kinase Kinase Kinase 5

ST1642
  
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      Übersicht

      Replacement Information

      Key Spec Table

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      M
      Description
      Overview

      This product has been discontinued.





      Recognizes the ~100 kDa MAP4K5 protein.
      Catalogue NumberST1642
      Brand Family Calbiochem®
      SynonymsAnti-Mitogen-Activated Protein Kinase Kinase Kinase Kinase 5
      Application Data
      Detection of human MAP4K5 by immunoblotting. Sample: Whole cell lysate from 293T cells transfected with MAP4K5 (lane 1) and from non-transfected 293T cells (lane 2). Primary antibody: Anti-MAP4K5 Mouse mAb (3H3) (Cat. No. ST1642) (1-5 µg/ml). Detection: chemiluminescence.
      References
      Product Information
      FormLiquid
      FormulationIn PBS, pH 7.2.
      PreservativeNone
      Applications
      Key Applications Enzyme-Linked Immunosorbent Assay
      Immunoblotting (Western Blotting)
      Application NotesELISA (see comments)
      Immunoblotting (1-5 µg/ml)
      Application CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
      Biological Information
      ImmunogenA recombinant polypeptide corresponding to amino acids of 361-470 human MAP4K5, expressed as GST fusion protein
      Clone3H3
      HostMouse
      IsotypeIgG₁
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Regulatory Review
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Bestellnummer GTIN
      ST1642 0

      Documentation

      Datenblatt

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision10-November-2010 RFH
      SynonymsAnti-Mitogen-Activated Protein Kinase Kinase Kinase Kinase 5
      ApplicationELISA (see comments)
      Immunoblotting (1-5 µg/ml)
      Application Data
      Detection of human MAP4K5 by immunoblotting. Sample: Whole cell lysate from 293T cells transfected with MAP4K5 (lane 1) and from non-transfected 293T cells (lane 2). Primary antibody: Anti-MAP4K5 Mouse mAb (3H3) (Cat. No. ST1642) (1-5 µg/ml). Detection: chemiluminescence.
      DescriptionPurified mouse monoclonal antibody. Recognizes the ~100 kDa MAP4K5 protein.
      BackgroundThis gene encodes a member of the serine/threonine protein kinase family, that is highly similar to yeast SPS1/STE20 kinase. Yeast SPS1/STE20 functions near the beginning of the MAP kinase signal cascades that is essential for yeast pheromone response. This kinase was shown to activate Jun kinase in mammalian cells, which suggested a role in stress response. Two alternatively spliced transcript variants encoding the same protein have been described for this gene.
      HostMouse
      ImmunogenA recombinant polypeptide corresponding to amino acids of 361-470 human MAP4K5, expressed as GST fusion protein
      Clone3H3
      IsotypeIgG₁
      Specieshuman
      FormLiquid
      FormulationIn PBS, pH 7.2.
      PreservativeNone
      CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Regulatory Review