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MABE215 Anti-Liver receptor homolog-1 Antibody, clone 1LRH-2E1

Use Anti-LRH-1 Antibody, clone 1LRH-2E1 (Mouse Monoclonal Antibody) validated in WB, ICC to detect LRH-1 also known as Liver receptor homolog 1.

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Research Category: Epigenetics & Nuclear Function Gene Symbol: Nr5a2, Lrh1 UniProt Number: P45448
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MABE215
100 µL  
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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      MWB, ICCMAscitesMonoclonal Antibody
      Description
      Catalogue NumberMABE215
      DescriptionAnti-Liver receptor homolog-1 Antibody, clone 1LRH-2E1
      Alternate Names
      • Nuclear receptor subfamily 5 group A member 2
      • Liver receptor homolog 1
      • LRH-1
      • NR5A2
      Background InformationLiver receptor homolog 1 (LRH-1, CPF, FTF, PHR-1, FTF, or hB1F) is an orphan nuclear receptor that is highly expressed in the intestines, pancreas, ovary, and liver. LRH-1 is a constitutively active transcription factor with a complex role in many cellular processes which may include lipid metabolism, cell proliferation, development, reproduction, and immunity. LRH-1 may interact with an array of transcriptional coactivators that modulate its activity, among which are the MBF1, SRC, and PGC-1α proteins. In addition, ERK phosphorylation on Ser-238 and Ser-243 also enhances LRH-1 activity. However, LRH-1 is also negatively regulated by several factors. For instance, corepressors such as SHP, DAX-1, and PROX-1, preclude its binding to DNA; and sumoylation promotes its inactivation and sequestration into nuclear bodies. LRH-1 targets many genes including the CYP7A1 and CYP8B1 genes involved in bile synthesis; and Oct4 required for pluripotency in embryonic stem cells. LRH-1 has been implicated in inflammatory bowel disease, and tumor development in the intestines and pancreas.
      References
      Product Information
      FormatAscites
      Control
      • Mouse pancreas tissue lysate
      PresentationMouse monoclonal IgG2aλ ascites with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationUse Anti-LRH-1 Antibody, clone 1LRH-2E1 (Mouse Monoclonal Antibody) validated in WB, ICC to detect LRH-1 also known as Liver receptor homolog 1.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      Application NotesWestern Blot Analysis: A 1:1,000 dilution from representative lot detected Liver receptor homolog-1 in HepG2 and HeLa cell lysates (M. Oulad, IGBMC, France.).

      Immunocytochemistry Analysis: A representative lot detected Liver receptor homolog-1 in HepG2 and HeLa cells (M. Oulad, IGBMC, France.).
      Biological Information
      ImmunogenLinear peptide corresponding to the mouse Liver receptor homolog-1.
      Clone1LRH-2E1
      HostMouse
      Species Reactivity
      • Mouse
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Nr5a2
      • Lrh1
      Purification MethodUnpurified
      UniProt Number
      Molecular Weight~64 kDa, ~56 kDa, and ~42 kDa observed. This antibody detects three isoforms of Liver receptor homolog-1 as described by Uniprot No. O00482.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in mouse pancreas tissue lysate.

      Western Blot Analysis: A 1:1,000 dilution of this antibody detected Liver receptor homolog-1 in 10 µg of mouse pancrease tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Bestellnummer GTIN
      MABE215 04053252522215

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      Kategorien

      Life Science Research > Antibodies and Assays > Primary Antibodies