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MABT538-AF488 Anti-Lamin A/C Antibody, clone 2E8.2 Antibody, Alexa Fluor® 488 Conjugate

This mouse monoclonal Anti-Lamin A/C Antibody, clone 2E8.2, Alexa Fluor® 488 Conjugate, Cat. No. MABT538-AF488 is used for the detection of Lamin A/C by Immunocytochemistry.

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Research Category: Cell Structure Gene Symbol: LMNA, LMN1 UniProt Number: P02545
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MABT538-AF488
100 µL  
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      Übersicht

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HICCMAlexaFluor®488Monoclonal Antibody
      Description
      Catalogue NumberMABT538-AF488
      DescriptionAnti-Lamin A/C Antibody, clone 2E8.2 Antibody, Alexa Fluor® 488 Conjugate
      Alternate Names
      • 70 kDa lamin
      • Renal carcinoma antigen NY-REN-32
      Background InformationLamin-A/C (UniProt: P02545; also known as 70 kDa lamin, Renal carcinoma antigen NY-REN-32) is encoded by the LMNA (also known as LMN1) gene (gene ID: 4000) in human. Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin. Proteolytic cleavage of 18 residues from the C-terminal of prelamin-A/C results in the production of lamin-A/C. Proteolytic cleavage requires prior farnesylation and methylation, and absence of these blocks cleavage. The prelamin-A/C maturation pathway includes farnesylation of CAAX motif, ZMPSTE24/FACE1 mediated cleavage of the last three amino acids, methylation of the C-terminal cysteine and endoproteolytic removal of the last 15 C-terminal amino acids. Farnesylation of prelamin-A/C also facilitates nuclear envelope targeting. Lamin A and C are present in equal amounts in the lamina of mammals. Lamin plays an important role in nuclear assembly, chromatin organization, nuclear membrane and telomere dynamics. Lamin C differs from canonical form A in the following manner: amino acids 567-572 are changed from GSHCSS to VSGSRR and form C does not amino acids 573-664 seen in canonical form A.
      References
      Product Information
      FormatAlexaFluor®488
      PresentationPurified mouse monoclonal antibody in PBS with 1.5% BSA and 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationThis mouse monoclonal Anti-Lamin A/C Antibody, clone 2E8.2, Alexa Fluor® 488 Conjugate, Cat. No. MABT538-AF488 is used for the detection of Lamin A/C by Immunocytochemistry.
      Key Applications
      • Immunocytochemistry
      Application NotesImmunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Lamin A/C in A431 cells.

      The unconjugated antibody (Cat. No. MABT538) is shown to be suitable also for Flow Cytometry, Immunohistochemistry, and Immunoprecipitation applications.
      Biological Information
      ImmunogenGST/His-tagged recombinant fragment corresponding to 109 amino acids from the C-terminal half of human Lamin A/C.
      Clone2E8.2
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone clone 2E8.2 specifically recognizes Lamin A/C in HeLa cells. This antibody has been shown to bind to an epitope between amino acids 464-572 in the C-terminal half.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • LMNA
      • LMN1
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight74.14 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunocytochemistry in HeLa cells.

      Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected Lamin A/C in HeLa cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Bestellnummer GTIN
      MABT538-AF488 04054839167546