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96-Well Plate
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48-602MAG
Buffer Detection Kit for Magnetic Beads
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06-017
Sigma-AldrichAnti-LRP6 Antibody, CT
This Anti-LRP6 Antibody, C-Terminus is validated for use in IH(P), WB for the detection of LRP6.
More>>This Anti-LRP6 Antibody, C-Terminus is validated for use in IH(P), WB for the detection of LRP6. Less<<
Anti-LRP6 Antibody, CT: SDB (Sicherheitsdatenblätter), Analysenzertifikate und Qualitätszertifikate, Dossiers, Broschüren und andere verfügbare Dokumente.
Low density lipoprotein receptor-related protein 6
Background Information
Low density lipoprotein receptor-related protein 6, also known as LRP6, is a member of the low density Lipoprotein receptor gene family (LDLR). LDLR are cell surface proteins. LRP6 plays a crucial role in embryonic development by functioning as a co-receptor for the Wnt signaling pathway. LRP6 is highly homologous and presents itself as a dimer in the cell membrane until it is phosphlorylated by Wnt. LRP6 has a high affinity with the Dickkopf (DKK) family of antagonists which, when bound, block Wnt. When LRP6 binds to Wnt it is phosphlorated at multiple sites, which results in Axin, a scaffolding protein, being recruited to the cell membrane and β-catenin activation. β-catenin prevents Wnt and LRP6 from binding. LRP6 is associated with autosomal dominant coronary artery disease type 2. Mutations resulting in the omission of the extracellular domain of LPR6 results in constant activity whereas carboxy intracellular domain exclusion has a negative effect, resulting in the blocking of Wnt signaling.
References
Product Information
Format
Purified
Control
Malignant melanocytes, fibrous muscularis surrounding ovarian cancer tissue.
Presentation
Purified rabbit polyclonal in buffer containing PBS with 0.09% sodium azide.
Applications
Application
This Anti-LRP6 Antibody, C-Terminus is validated for use in IH(P), WB for the detection of LRP6.
Key Applications
Immunohistochemistry (Paraffin)
Western Blotting
Application Notes
Western Blot Analysis: A previous lot of this antibody was used in western blot on 293 cell lysate.
Biological Information
Immunogen
KLH conjugated linear peptide corresponding to the C-terminus of LRP6.
Epitope
Antibody recognizes C-terminus of LRP6.
Concentration
Please refer to the Certificate of Analysis for the lot-specific concentration.
The LRP6 gene encodes a member of the low density lipoprotein receptor (LDLR) gene family, which consists of cell surface proteins involved in receptor-mediated endocytosis of specific ligands. LDLR proteins are comprised of the same basic structural motifs: an extracellular domain that contains LDLR binding repeats and EGF repeats with associated spacer domains containing the YWTD motif; a single transmembrane-spanning domain; and a C-terminal cytoplasmic domain that generally contains at least 1 copy of the NPXY motif (Brown et al., 1998 [PubMed 9704021]). LRP6 and LRP5 (MIM 603506) function as coreceptors for WNT (MIM 164820) ligands and thus play a role in WNT signaling, which is important in a wide variety of biologic processes during pre- and postnatal life in invertebrates and vertebrates (Kokubu et al., 2004 [PubMed 15469977]).[supplied by OMIM]
Function: Essential for the Wnt/beta catenin signaling pathway, probably by acting as a coreceptor together with Frizzled for Wnt. Specific high-affinity receptor for DKK1 and DKK2, but not DKK3. The interaction with DKK1 blocks LRP6-mediated Wnt/beta catenin signaling via LRP6 removal via Kremen proteins-mediated endocytosis. Ref.3 Subunit structure Interacts with RSPO1 and RSPO3 By similarity. Interacts with FZD5. Essential component of the Wnt7 receptor complex. Wnt7A interacts with the LRP6/FZD5 complex. This interaction is antagonized by DKK1 and DKK3. Subcellular location: Membrane; Single-pass type I membrane proteinPotential. Tissue specificity Widely co-expressed with LRP5 during embryogenesis and in adult tissues. Domain: The YWTD-EGF-like domains 1 and 2 are required for the interaction with Wnt-frizzled complex. The YWTD-EGF-like domains 3 and 4 are required for the interaction with DKK1. Ref.3 Involvement in disease: Defects in LRP6 are the cause of autosomal dominant coronary artery disease type 2 (ADCAD2) [MIM:610947]. Ref.6 Sequence similarities: Belongs to the LDLR family. Contains 4 EGF-like domains. Contains 3 LDL-receptor class A domains. Contains 20 LDL-receptor class B repeats.
Molecular Weight
~181 kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance
Routinely evaluated by immunohistochemistry on fibrous muscularis surrounding ovarian cancer tissue.
Immunohistochemistry (paraffin): Representative lot data. Optimal Staining with Citrate Buffer, pH 6.0, epitope retrieval: Human ovarian cancer tissue.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
