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ABS1634 Anti-LIP5

Detect LIP5 (Vacuolar protein sorting-associated protein VTA1 homolog), using this rabbit polyclonal Anti-LIP5, Cat. No. ABS1634, validated for use in Immunocytochemistry and Western Blotting.

Anti-LIP5: SDB (Sicherheitsdatenblätter), Analysenzertifikate und Qualitätszertifikate, Dossiers, Broschüren und andere verfügbare Dokumente.
Research Category: Signaling Gene Symbol: VTA1, C6orf55, HSPC228, My012 UniProt Number: Q9NP79
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ABS1634
100 µL  
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      Übersicht

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, ICCRbSerumPolyclonal Antibody
      Description
      Catalogue NumberABS1634
      DescriptionAnti-LIP5
      Alternate Names
      • Vacuolar protein sorting-associated protein VTA1 homolog
      • Dopamine-responsive gene 1 protein
      • DRG-1
      • LYST-interacting protein 5
      • LIP5
      • SKD1-binding protein 1
      • SBP1
      Background InformationVacuolar protein sorting-associated protein VTA1 homolog (UniProt: Q9NP79; also known as Dopamine-responsive gene 1 protein; DRG-1; LYST-interacting protein 5; LIP5; SKD1-binding protein 1; SBP1) is encoded by the VTA1 (also known as C6orf55; HSPC228; My012) gene (Gene ID 51534) in human. LIP5 is a 33.9 kDa protein involved in trafficking of the multi-vesicular body. It helps regulate the membrane fission and recycling activities of the ESCRT pathway. It performs its central regulatory function within the ESCRT pathway by binding ESCRT-III proteins through its N-terminal tandem microtubule-interacting and trafficking (MIT) domain and by binding VPS4 ATPases through its C-terminal VSL (Vta1/SBP1/LIP5) domain. These interactions allow LIP5 to coordinate ESCRT-III and VPS4 activities both during normal cellular homeostasis and also in response to interferon signaling during innate immune responses. The ESCRT-III and VPS4 ATPase complexes catalyze the membrane fission events associated with these processes. The LIP5 protein helps regulate their interactions by binding directly to a subset of ESCRT-III proteins and to VPS4.
      References
      Product Information
      FormatSerum
      PresentationRabbit polyclonal antibody serum with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationDetect LIP5 (Vacuolar protein sorting-associated protein VTA1 homolog), using this rabbit polyclonal Anti-LIP5, Cat. No. ABS1634, validated for use in Immunocytochemistry and Western Blotting.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      Application NotesImmunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected LIP5 in HeLa cells.

      Western Blotting Analysis: A representative lot detected endogenous LIP5 co-immunoprecipiated with Myc-tagged VPS4A from HEK293T cells (Skalicky, J.J., et al. (2012). J Biol Chem. 287(52):43910-43926).
      Biological Information
      ImmunogenHis-tagged full length human recombinant LIP5.
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityDetects LIP5 (Vacuolar protein sorting-associated protein VTA1 homolog) in HeLa cells
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • VTA1
      • C6orf55
      • HSPC228
      • My012
      Purification MethodUnpurified.
      UniProt Number
      Molecular Weight~38 kDa observed. 33.88 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HeLa cell lysate.

      Western Blotting Analysis: A 1:5,000 dilution of this antiserum detected LIP5 in HeLa cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.

      Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Bestellnummer GTIN
      ABS1634 04054839135262