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48-602MAG
Buffer Detection Kit for Magnetic Beads
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Purified from mouse ascitic fluid. Fluorescein conjugated. The antibody is supplied in 0.5ml phosphate-buffered saline, pH 7.4, containing 0.2% bovine serum albumin and 0.1% sodium azide. The characteristics of each lot are tested by flow cytometry.
Anti-HLA-B27 Antibody, clone HLA.ABC.m3, FITC conjugated detects level of HLA-B27 & has been published & validated for use in FC & IF.
Key Applications
Flow Cytometry
Immunofluorescence
Application Notes
This antibody is useful in the investigation of HLA-B27 in diseases such as ankylosing spondylitis and anterior uveitis.
SUGGESTED USAGE DILUTION
Flow cytometry and immunofluorescence 1:15 to 1:60
Dilute with isotonic buffer. Use 50 μl of diluted antibody per 100 μl of whole blood, or 1 x 10E6 peripheral blood mononuclear cells (PBMC) in 100 μl buffer.
Biological Information
Immunogen
Immune complex precipitated from HLA-B27 positive cell line (Bordin) by anti HLA antibody and staphylococcal Protein A.
Clone
HLA.ABC.m3
Concentration
Please refer to the Certificate of Analysis for the lot-specific concentration.
Host
Mouse
Specificity
This antibody binds strongly to the HLA-B27 antigen. It also binds less strongly to the HLA-B7 antigen. Affinity studies by Scatchard analysis showed that the antibody has a higher affinity for HLA-B27 (9.7x108M-1) than for HLA-B7
HLA-B belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. They are expressed in nearly all cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon 1 encodes the leader peptide, exon 2 and 3 encode the alpha1 and alpha2 domains, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region and exons 6 and 7 encode the cytoplasmic tail. Polymorphisms within exon 2 and exon 3 are responsible for the peptide binding specificity of each class one molecule. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. Hundreds of HLA-B alleles have been described.
FUNCTION: SwissProt: P30498 # Involved in the presentation of foreign antigens to the immune system.| Q31612 # Involved in the presentation of foreign antigens to the immune system.| P30488 # Involved in the presentation of foreign antigens to the immune system.| P03989 # Involved in the presentation of foreign antigens to the immune system.| P30493 # Involved in the presentation of foreign antigens to the immune system.| P30460 # Involved in the presentation of foreign antigens to the immune system.| P30484 # Involved in the presentation of foreign antigens to the immune system.| P01889 # Involved in the presentation of foreign antigens to the immune system.| P30485 # Involved in the presentation of foreign antigens to the immune system.| Q29940 # Involved in the presentation of foreign antigens to the immune system.| P30483 # Involved in the presentation of foreign antigens to the immune system.| P30495 # Involved in the presentation of foreign antigens to the immune system.| P30492 # Involved in the presentation of foreign antigens to the immune system.| P18465 # Involved in the presentation of foreign antigens to the immune system.| P30487 # Involved in the presentation of foreign antigens to the immune system.| P30480 # Involved in the presentation of foreign antigens to the immune system.| P30491 # Involved in the presentation of foreign antigens to the immune system.| Q95365 # Involved in the presentation of foreign antigens to the immune system.| Q29836 # Involved in the presentation of foreign antigens to the immune system.| Q29718 # Involved in the presentation of foreign antigens to the immune system.| P30475 # Involved in the presentation of foreign antigens to the immune system.| P18464 # Involved in the presentation of foreign antigens to the immune system.| P30479 # Involved in the presentation of foreign antigens to the immune system.| P30490 # Involved in the presentation of foreign antigens to the immune system.| P10319 # Involved in the presentation of foreign antigens to the immune system.| P30466 # Involved in the presentation of foreign antigens to the immune system.| P30461 # Involved in the presentation of foreign antigens to the immune system.| P18463 # Involved in the presentation of foreign antigens to the immune system.| Q04826 # Involved in the presentation of foreign antigens to the immune system.| P30481 # Involved in the presentation of foreign antigens to the immune system.| P30685 # Involved in the presentation of foreign antigens to the immune system.| P30462 # Involved in the presentation of foreign antigens to the immune system.| Q31610 # Involved in the presentation of foreign antigens to the immune system.| P30486 # Involved in the presentation of foreign antigens to the immune system.| P30464 # Involved in the presentation of foreign antigens to the immune system.
SIZE: 362 amino acids; 40478 Da
SUBUNIT: Heterodimer of an alpha chain and a beta chain (beta-2- microglobulin). Interacts with human herpesvirus 8 MIR1 protein (By similarity).
SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
DOMAIN: SwissProt: P30498
PTM: Polyubiquitinated in a post ER compartment by interaction with human herpesvirus 8 MIR1 protein. This targets the protein for rapid degradation via the ubiquitin system (By similarity).
DISEASE: "SwissProt: P03989 # HLA-B27 is associated with the development of ankylosing spondylitis (AS) [MIM:106300]. AS is a chronic inflammatory rheumatic disease that mainly affects the axial skeleton and is considered the prototype of seronegative spondyloarthropathies (SNSA), which include reactive arthritis (e.g., Reiter's syndrome), psoritic spondylitis, and other spondyloarthropathies (SpA). In the Greek Cypriot population, a restricted number of HLA-B27 subtypes are associated with AS and other B27-related diseases and an elevated frequency of the B*2702 allele in the AS patients is identified. The allele B*2707 seems to have a protective role in this population because it was found only in the healthy controls.| P18464 # HLA-B51 is associated with Behcet disease but it is not certain whether HLA-B51 itself or a closely linked gene is responsible for susceptibility. About 20% of healthy individuals of various ethnic origin carries the HLA-B 51 locus, compared with 50 to 80% of patients. Behcet disease is a systemic disorder of recurrent acute inflammation characterized by 4 major symptoms: oral aphthous ulcers, skin lesions, ocular symptoms and genital ulcerations. Occasionally, inflammation in tissues and organs throughout the body can occur, including the gastrointestinal tract, central nervous system, vascular system, lungs, and kidneys. The etiology of Behcet disease is unclear.| P30464 # Involved in susceptibility to Stevens-Johnson syndrome [MIM:608579]. This is a life-threatening reaction of the skin to particular types of medication. It is characterized by high fever, malaise and blistering exanthema. The more severe form of the disease is referred to as toxic epidermal necrolysis."
SIMILARITY: SwissProt: P30498 ## Belongs to the MHC class I family. & Contains 1 Ig-like C1-type (immunoglobulin-like) domain. | Q31612 ## Belongs to the MHC class I family. & Contains 1 Ig-like C1-type (immunoglobulin-like) domain. | P30488 ## Belongs to the MHC class I family. & Contains 1 Ig-like C1-type (immunoglobulin-like) domain. | P03989 ## Belongs to the MHC class I family. & Contains 1 Ig-like C1-type (immunoglobulin-like) domain. | P30493 ## Belongs to the MHC class I family. & Contains 1 Ig-like C1-type (immunoglobulin-like) domain. | P30460 ## Belongs to the MHC class I family. & Contains 1 Ig-like C1-type (immunoglobulin-like) domain. | P30484 ## Belongs to the MHC class I family. & Contains 1 Ig-like C1-type (immunoglobulin-like) domain. | P01889 ## Belongs to the MHC class I family. & Contains 1 Ig-like C1-type (immunoglobulin-like) domain. | P30485 ## Belongs to the MHC class I family. & Contains 1 Ig-like C1-type (immunoglobulin-like) domain. | Q29940 ## Belongs to the MHC class I family. & Contains 1 Ig-like C1-type (immunoglobulin-like) domain. | P30483 ## Belongs to the MHC class I family. & Contains 1 Ig-like C1-type (immunoglobulin-like) domain. | P30495 ## Belongs to the MHC class I family. & Contains 1 Ig-like C1-type (immunoglobulin-like) domain. | P30492 ## Belongs to the MHC class I family. & Contains 1 Ig-like C1-type (immunoglobulin-like) domain. | P18465 ## Belongs to the MHC class I family. & Contains 1 Ig-like C1-type (immunoglobulin-like) domain. | P30487 ## Belongs to the MHC class I family. & Contains 1 Ig-like C1-type (immunoglobulin-like) domain. | P30480 ## Belongs to the MHC class I family. & Contains 1 Ig-like C1-type (immunoglobulin-like) domain. | P30491 ## Belongs to the MHC class I family. & Contains 1 Ig-like C1-type (immunoglobulin-like) domain. | Q95365 ## Belongs to the MHC class I family. & Contains 1 Ig-like C1-type (immunoglobulin-like) domain. | Q29836 ## Belongs to the MHC class I family. & Contains 1 Ig-like C1-type (immunoglobulin-like) domain. | Q29718 ## Belongs to the MHC class I family. & Contains 1 Ig-like C1-type (immunoglobulin-like) domain. | P30475 ## Belongs to the MHC class I family. & Contains 1 Ig-like C1-type (immunoglobulin-like) domain. | P18464 ## Belongs to the MHC class I family. & Contains 1 Ig-like C1-type (immunoglobulin-like) domain. | P30479 ## Belongs to the MHC class I family. & Contains 1 Ig-like C1-type (immunoglobulin-like) domain. | P30490 ## Belongs to the MHC class I family. & Contains 1 Ig-like C1-type (immunoglobulin-like) domain. | P10319 ## Belongs to the MHC class I family. & Contains 1 Ig-like C1-type (immunoglobulin-like) domain. | P30466 ## Belongs to the MHC class I family. & Contains 1 Ig-like C1-type (immunoglobulin-like) domain. | P30461 ## Belongs to the MHC class I family. & Contains 1 Ig-like C1-type (immunoglobulin-like) domain. | P18463 ## Belongs to the MHC class I family. & Contains 1 Ig-like C1-type (immunoglobulin-like) domain. | Q04826 ## Belongs to the MHC class I family. & Contains 1 Ig-like C1-type (immunoglobulin-like) domain. | P30481 ## Belongs to the MHC class I family. & Contains 1 Ig-like C1-type (immunoglobulin-like) domain. | P30685 ## Belongs to the MHC class I family. & Contains 1 Ig-like C1-type (immunoglobulin-like) domain. | P30462 ## Belongs to the MHC class I family. & Contains 1 Ig-like C1-type (immunoglobulin-like) domain. | Q31610 ## Belongs to the MHC class I family. & Contains 1 Ig-like C1-type (immunoglobulin-like) domain. | P30486 ## Belongs to the MHC class I family. & Contains 1 Ig-like C1-type (immunoglobulin-like) domain. | P30464 ## Belongs to the MHC class I family. & Contains 1 Ig-like C1-type (immunoglobulin-like) domain.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Store at 2 to 8°C. DO NOT FREEZE. Protect from light.
WARNING: The monoclonal reagent solution contains 0.1% sodium azide as a preservative. Due to potential hazards arising from the build up of this material in pipes, spent reagent should be disposed of with liberal volumes of water.
The production and characterization of a new anti-HLA-B27 monoclonal antibody HLA-ABC-m3 is described. This cytotoxic IgG2a antibody binds protein A and is able to precipitate cell surface molecules of 43,000 and 12,000 daltons corresponding to the HLA heavy chain and beta 2-microglobulin. Population testing revealed that the HLA-ABC-m3 antibody reacted with the peripheral blood lymphocytes of 47/47 individuals conventionally typed as HLA-B27+ and with 5/105 HLA-B27- individuals. These five extra reactions were with individuals expressing the cross-reactive HLA-B7 alloantigen, although the affinity of the monoclonal antibody for B27 heterozygous individuals (approx 10(9) M-1) was tenfold greater than with B7 individuals (approx 10(8) M-1). In addition, HLA-ABC-m3 reactivity segregated with HLA-B27 in two families. This monoclonal antibody should be of value in the investigation of the role of HLA-B27 in disease.
