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DR1067 Anti-GTF2I Mouse mAb (3E2)

Anti-GTF2I Mouse mAb (3E2): SDB (Sicherheitsdatenblätter), Analysenzertifikate und Qualitätszertifikate, Dossiers, Broschüren und andere verfügbare Dokumente.

Synonyme: Anti-General Transcription Factor IIi

DR1067
  
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      Replacement Information

      Key Spec Table

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      Description
      Overview

      This product has been discontinued.





      Recognizes the ~130 kDa GTF2I protein in HeLa cells and human colon and placenta tissue.
      Catalogue NumberDR1067
      Brand Family Calbiochem®
      SynonymsAnti-General Transcription Factor IIi
      Application Data
      Detection of human GTF2I by immunoblotting. Sample: Whole tissue extract from human colon. Primary antibody: Anti-GTF2I Mouse mAb (3E2) (Cat. No. DR1067) (1-5 µg/ml). Detection: chemiluminescence.

      Detection of human GTF2I by immunocytochemistry. Sample: HeLa cells fixed in paraformaldehyde. Primary antibody: Anti-GTF2I Mouse mAb (3E2) (Cat. No. DR1067) (10 µg/ml). Detection: fluorescence.

      Detection of human GTF2I by staining paraffin sections. Sample: Formalin-fixed, paraffin-embedded human placenta tissue. Primary antibody: Anti-GTF2I Mouse mAb (3E2) (Cat. No. DR1067) (3 µg/ml). Detection: colorimetric (DAB).
      References
      Product Information
      FormLiquid
      FormulationIn PBS, pH 7.11.
      Positive controlHuman colon tissue, Human placenta tissue, HeLa cells
      PreservativeNone
      Applications
      Key Applications Enzyme-Linked Immunosorbent Assay
      Immunoblotting (Western Blotting)
      Immunocytochemistry
      Paraffin Sections
      Application NotesELISA (1-5 µg/ml)
      Immunoblotting (1-5 µg/ml)
      Immunocytochemistry (10 µg/ml)
      Paraffin Sections (3 µg/ml)
      Application CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
      Biological Information
      ImmunogenFull-length, recombinant, human GTF2l (aa 36-275), expressed as a GST fusion protein
      Clone3E2
      HostMouse
      IsotypeIgG2a
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Regulatory Review
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Bestellnummer GTIN
      DR1067 0

      Documentation

      Datenblatt

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision04-November-2010 RFH
      SynonymsAnti-General Transcription Factor IIi
      ApplicationELISA (1-5 µg/ml)
      Immunoblotting (1-5 µg/ml)
      Immunocytochemistry (10 µg/ml)
      Paraffin Sections (3 µg/ml)
      Application Data
      Detection of human GTF2I by immunoblotting. Sample: Whole tissue extract from human colon. Primary antibody: Anti-GTF2I Mouse mAb (3E2) (Cat. No. DR1067) (1-5 µg/ml). Detection: chemiluminescence.

      Detection of human GTF2I by immunocytochemistry. Sample: HeLa cells fixed in paraformaldehyde. Primary antibody: Anti-GTF2I Mouse mAb (3E2) (Cat. No. DR1067) (10 µg/ml). Detection: fluorescence.

      Detection of human GTF2I by staining paraffin sections. Sample: Formalin-fixed, paraffin-embedded human placenta tissue. Primary antibody: Anti-GTF2I Mouse mAb (3E2) (Cat. No. DR1067) (3 µg/ml). Detection: colorimetric (DAB).
      DescriptionPurified mouse monoclonal antibody. Recognizes the ~130 kDa GTF2I protein.
      BackgroundGTF2l is required for the formation of functional ARID3A DNA-binding complexes and for activation of immunoglobulin heavy-chain transcription upon B-lymphocyte activation.
      HostMouse
      ImmunogenFull-length, recombinant, human GTF2l (aa 36-275), expressed as a GST fusion protein
      Clone3E2
      IsotypeIgG2a
      Specieshuman
      Positive controlHuman colon tissue, Human placenta tissue, HeLa cells
      FormLiquid
      FormulationIn PBS, pH 7.11.
      PreservativeNone
      CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Regulatory Review